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1.
Maya D. Glinchuk Vladislav Skorokhod Igor Bykov Vilnis Dimza Eva erno kova Toma Kala 《Ferroelectrics Letters Section》1994,18(5):191-196
X-band EPR spectra of PLZT 1/65/35 and PLZT 8/65/35 doped with 1% FE3+ were recorded at temperature range -175°C to 200°C. Three types of paramagnetic centers were found. Two of them are in strong axial and rhombic crystal field due to neighboring oxygen and lead vacancies. The other center has symmetry determined by polarization and oxygen octahedra tilt. EPR spectra of PLZT 8/65/35 are in agreement with its glassy behavior. 相似文献
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Delphine Vincent AnhDuyen Bui Doris Ram Vilnis Ezernieks Frank Bedon Joe Panozzo Pankaj Maharjan Simone Rochfort Hans Daetwyler Matthew Hayden 《International journal of molecular sciences》2022,23(2)
Bread wheat is the most widely cultivated crop worldwide, used in the production of food products and a feed source for animals. Selection tools that can be applied early in the breeding cycle are needed to accelerate genetic gain for increased wheat production while maintaining or improving grain quality if demand from human population growth is to be fulfilled. Proteomics screening assays of wheat flour can assist breeders to select the best performing breeding lines and discard the worst lines. In this study, we optimised a robust LC–MS shotgun quantitative proteomics method to screen thousands of wheat genotypes. Using 6 cultivars and 4 replicates, we tested 3 resuspension ratios (50, 25, and 17 µL/mg), 2 extraction buffers (with urea or guanidine-hydrochloride), 3 sets of proteases (chymotrypsin, Glu-C, and trypsin/Lys-C), and multiple LC settings. Protein identifications by LC–MS/MS were used to select the best parameters. A total 8738 wheat proteins were identified. The best method was validated on an independent set of 96 cultivars and peptides quantities were normalised using sample weights, an internal standard, and quality controls. Data mining tools found particularly useful to explore the flour proteome are presented (UniProt Retrieve/ID mapping tool, KEGG, AgriGO, REVIGO, and Pathway Tools). 相似文献
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Thethermodynamic state function free energy F(T,V,N) as function of the cluster distribution N and the thermodynamic parameters (temperature T and volume V) is calculated in the canonical ensemble. With the help of the Legendre transformation we get all other state functions for different boundary conditions. Monte Carlo simulations with 15000 particles starting from metastable homogeneous initial conditions are made for water and methanol. The Bethe-Weizsäcker ansatz and the Padé approximation are used for the binding energy of clusters. A first-order phase transition is observed under appropriate conditions. 相似文献
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The influence of vaccine genotype and route of administration on the efficacy of pseudorabies virus (PRV) vaccines against virulent PRV challenge was evaluated in a controlled experiment using five genotypically distinct modified live vaccines (MLVs) for PRV. Several of these MLVs share deletions in specific genes, however, each has its deletion in a different locus within that gene. Pigs were vaccinated with each vaccine, either via the intramuscular or intranasal route, and subsequently challenged with a highly virulent PRV field strain. During a 2-week period following challenge with virulent PRV, each of the vaccine strains used in this study was evaluated for its effectiveness in the reduction of clinical signs, prevention of growth retardation and virulent virus shedding. One month after challenge, tissues were collected and analyzed for virulent PRV latency load by a recently developed method for the electrochemiluminescent quantitation of latent herpesvirus DNA in animal tissues after PCR amplification. It was determined that all vaccination protocols provided protection against clinical signs resulting from field virus challenge and reduced both field virus shedding and latency load after field virus challenge. Our results indicated that vaccine efficacy was significantly influenced by the modified live vaccine strain and route of administration. Compared to unvaccinated pigs, vaccination reduced field virus latency load in trigeminal ganglia, but significant differences were found between vaccines and routes of administration. We conclude that vaccine genotype plays a role in the effectiveness of PRV MLVs. 相似文献
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Amir Jourak Vilnis Frishfelds T. Staffan Lundström Inga Herrmann Annelie Hedström 《American Institute of Chemical Engineers》2013,59(3):1002-1011
The longitudinal (DL) and transverse (DT) dispersion coefficients in two‐dimensional (2‐D) randomly packed beds of circular particles in a laminar flow regime are derived. A 2‐D discrete system of particles is divided into cells using modified Voronoi diagrams. The relationship between the variation of the stream function and the averaged vorticity is obtained from computational fluid dynamics (CFD) simulations. The whole flow pattern is then obtained by using the principle of energy dissipation rate minimization. The obtained values of DL agree well with 3‐D experimental data for all velocities investigated. At very high velocities, DT in 2‐D appears to be higher than 3‐D experimental data. In addition, the effects of particle‐size distributions, packing structure, and porosity on the DL and DT were studied. One result was that an increase in the width of the particle‐size distribution resulted in higher values of DL and DT at high velocities. © 2012 American Institute of Chemical Engineers AIChE J, 59: 1002–1011, 2013 相似文献