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1.
Wireless Personal Communications - For robust monitoring, control and proper energy management of renewable energy sources (RES), wireless sensing networks (WSNs) are proved to be a vital solution.... 相似文献
2.
Magdalena Misiura Tomasz Guszczyn Ilona Oscilowska Weronika Baszanowska Jerzy Palka Wojciech Miltyk 《International journal of molecular sciences》2021,22(2)
Although the role of platelet-rich plasma (PRP) in tissue regeneration has been confirmed in many studies, the mechanism of this process is still not fully understood. Human keratinocytes (HaCaT) cells were used as an experimental model for studies on the effects of PRP on cell proliferation, migration, collagen biosynthesis, prolidase activity, and its expression and anabolic signaling. The activation of epidermal growth factor receptor (EGFR), β1-integrin, and insulin-like growth factor-1 receptor (IGF-1R) by PRP were investigated by western blot and immunocytochemistry. It has been found that PRP induced keratinocytes migration and proliferation through activation of cell cycle progression and EGFR downstream signaling. Similar biological effects were achieved by an addition to the culture medium of prolidase (PEPD), a ligand of EGFR (PRP is a rich source of PEPD–2 ng/mL). PRP-dependent stimulation of collagen biosynthesis was accompanied by an increase in the expression of NF-κβ, IGF-1R-downstream signaling proteins, and PEPD activity. The data suggest that PRP activates a complex of growth factors and adhesion receptors that stimulate cell proliferation, migration, and collagen biosynthesis. PRP induces PEPD-dependent human keratinocyte proliferation through activation of the EGFR receptor. Our study provides a novel mechanism of PRP-dependent wound healing. 相似文献
3.
Siebert HC André S Asensio JL Cañada FJ Dong X Espinosa JF Frank M Gilleron M Kaltner H Kozár T Bovin NV von Der Lieth CW Vliegenthart JF Jiménez-Barbero J Gabius HJ 《Chembiochem : a European journal of chemical biology》2000,1(3):181-195
This study documents the feasibility of switching to an aprotic medium in sugar receptor research. The solvent change offers additional insights into mechanistic details of receptor--carbohydrate ligand interactions. If a receptor retained binding capacity in an aprotic medium, solvent-exchangeable protons of the ligand would not undergo transfer and could act as additional sensors, thus improving the level of reliability in conformational analysis. To probe this possibility, we first focused on hevein, the smallest lectin found in nature. The NMR-spectroscopic measurements verified complexation, albeit with progressively reduced affinity by more than 1.5 orders of magnitude, in mixtures of up to 50% dimethyl sulfoxide (DMSO). Since hevein lacks the compact beta-strand arrangement of other sugar receptors, such a structural motif may confer enhanced resistance to solvent exchange. Two settings of solid-phase activity assays proved this assumption for three types of alpha- and/or beta-galactoside-binding proteins, that is, a human immunoglobulin G (IgG) subfraction, the mistletoe lectin, and a member of the galectin family of animal lectins. Computer-assisted calculations and NMR experiments also revealed no conspicuous impact of the solvent on the conformational properties of the tested ligands. To define all possible nuclear Overhauser effect (NOE) contacts in a certain conformation and to predict involvement of exchangeable protons, we established a new screening protocol applicable during a given molecular dynamics (MD) trajectory and calculated population densities of distinct contacts. Experimentally, transferred NOE (tr-NOE) experiments with IgG molecules and the disaccharide Gal'alpha1-3Galbeta1-R in DMSO as solvent disclosed that such an additional crosspeak, that is, Gal'OH2--GalOH4, was even detectable for the bound ligand under conditions in which spin diffusion effects are suppressed. Further measurements with the plant lectin and galectins confirmed line broadening of ligand signals and gave access to characteristic crosspeaks in the aprotic solvent and its mixtures with water. Our combined biochemical, computational, and NMR-spectroscopical strategy is expected to contribute notably to the precise elucidation of the geometry of ligands bound to compactly folded sugar receptors and of the role of water molecules in protein--ligand (carbohydrate) recognition, with relevance to areas beyond the glycosciences. 相似文献
4.
Lucjan Janowski Piotr Kozłowski Remigiusz Baran Piotr Romaniak Andrzej Glowacz Tomasz Rusc 《Multimedia Tools and Applications》2014,68(1):23-40
Video transmission and analysis is often utilized in applications outside of the entertainment sector, and generally speaking this class of video is used to perform specific tasks. Examples of these applications include security and public safety. The Quality of Experience (QoE) concept for video content used for entertainment differs significantly from the QoE of surveillance video used for recognition tasks. This is because, in the latter case, the subjective satisfaction of the user depends on achieving a given functionality. Recognizing the growing importance of video in delivering a range of public safety services, we focused on developing critical quality thresholds in license plate recognition tasks based on videos streamed in constrained networking conditions. Since the number of surveillance cameras is still growing it is obvious that automatic systems will be used to do the tasks. Therefore, the presented research includes also analysis of automatic recognition algorithms. 相似文献
5.
The two types of high-sulphur coals Mequinenza and Illinois No. 6, in the initial form and subjected to potassium/liquid ammonia reduction, were analysed by atmospheric pressure-temperature programmed reduction (AP-TPR) method. It has been shown that preliminary demineralisation was beneficial for AP-TPR measurements because of the removal of calcium compounds. The reduction of sulphides and disulphides in the potassium/liquid ammonia system was found to lead to formation of aromatic and aliphatic thiols. The presence of the latter is better manifested in the AP-TPR kinetograms when the measurements are performed in the presence of a special reducing mixture. It has been shown that the coal reduction in the potassium/liquid ammonia system apart from transformations of non-thiophene sulphur groups also leads to breaking up of the C-S bonds in some thiophene systems. 相似文献
6.
Mobile context-based framework for threat monitoring in urban environment with social threat monitor
Szymon Bobek Grzegorz J. Nalepa Antoni Ligȩza Weronika T. Adrian Krzysztof Kaczor 《Multimedia Tools and Applications》2016,75(17):10595-10616
Engaging users in threat reporting is important in order to improve threat monitoring in urban environments. Today, mobile applications are mostly used to provide basic reporting interfaces. With a rapid evolution of mobile devices, the idea of context awareness has gained a remarkable popularity in recent years. Modern smartphones and tablets are equipped with a variety of sensors including accelerometers, gyroscopes, pressure gauges, light and GPS sensors. Additionally, the devices become computationally powerful which allows for real-time processing of data gathered by their sensors. Universal access to the Internet via WiFi hot-spots and GSM network makes mobile devices perfect platforms for ubiquitous computing. Although there exist numerous frameworks for context-aware systems, they are usually dedicated to static, centralized, client-server architectures. There is still space for research in the field of context modeling and reasoning for mobile devices. In this paper, we propose a lightweight context-aware framework for mobile devices that uses data gathered by mobile device sensors and performs on-line reasoning about possible threats based on the information provided by the Social Threat Monitor system developed in the INDECT project. 相似文献
7.
Krzysztof Kozłowski Przemysław Herman 《Journal of Intelligent and Robotic Systems》2008,53(3):205-221
In this paper we present new control algorithms for robots with dynamics described in terms of quasi-velocities (Kozłowski,
Identification of articulated body inertias and decoupled control of robots in terms of quasi-coordinates. In: Proc. of the
1996 IEEE International Conference on Robotics and Automation, pp. 317–322. IEEE, Piscataway, 1996a; Zeitschrift für Angewandte Mathematik und Mechanik 76(S3):479–480, 1996c; Robot control algorithms in terms of quasi-coordinates. In: Proc. of the 34 Conference on Decision and Control, pp. 3020–3025,
Kobe, 11–13 December 1996, 1996d). The equations of motion are written using spatial quantities such as spatial velocities, accelerations, forces, and articulated
body inertia matrices (Kozłowski, Standard and diagonalized Lagrangian dynamics: a comparison. In: Proc. of the 1995 IEEE
Int. Conf. on Robotics and Automation, pp. 2823–2828. IEEE, Piscataway, 1995b; Rodriguez and Kreutz, Recursive Mass Matrix Factorization and Inversion, An Operator Approach to Open- and Closed-Chain
Multibody Dynamics, pp. 88–11. JPL, Dartmouth, 1998). The forward dynamics algorithms incorporate new control laws in terms of normalized quasi-velocities. Two cases are considered:
end point trajectory tracking and trajectory tracking algorithm, in general. It is shown that by properly choosing the Lyapunov
function candidate a dynamic system with appropriate feedback can be made asymptotically stable and follows the desired trajectory
in the task space. All of the control laws have a new architecture in the sense that they are derived, in the so-called quasi-velocity
and quasi-force space, and at any instant of time generalized positions and forces can be recovered from order recursions, where denotes the number of degrees of freedom of the manipulator. This paper also contains the proposition of a sliding mode control,
originally introduced by Slotine and Li (Int J Rob Res 6(3):49–59, 1987), which has been extended to the sliding mode control in the quasi-velocity and quasi-force space. Experimental results illustrate
behavior of the new control schemes and show the potential of the approach in the quasi-velocity and quasi-force space.
Authors are with Chair of Control and Systems Engineering. 相似文献
8.
J. Marciak-Kozłowska Z. Mucha M. Kozłowski 《International Journal of Thermophysics》1995,16(6):1489-1497
In this paper it has been shown that, with the advent of lasers with a very short pulse duration, the effect of thermal wave propagation becomes important. To consider this effect, hyperbolic heat conduction in thin gold films was studied. It was shown that for heat fluxes of the order 108 W·cm–2, a thermal wave is generated in thin gold films. The consideration of the hyperbolicity of heat transfer enables one to describe the temperature profile with one value of fluence. 相似文献
9.
H Tlaskalová-Hogenová R St?pánková L Tucková MA Farré DP Funda EF Verdú J Sinkora T Hudcovic Z Reháková B Cukrowska H Kozáková L Prokesová 《Canadian Metallurgical Quarterly》1998,43(5):545-550
Despite the fact that target antigens and the genetic basis of several autoimmune diseases are now better understood, the initial events leading to a loss of tolerance towards self-components remain unknown. One of the most attractive explanations for autoimmune phenomena involves various infections as possible natural events capable of initiating the process in genetically predisposed individuals. The most accepted explanation of how infection causes autoimmunity is based on the concept of "molecular mimicry" (similarity between the epitopes of an autoantigen and the epitopes in the environmental antigen). Infectious stimuli may also participate in the development of autoimmunity by inducing an increased expression of stress proteins (hsp), chaperones and transplantation antigens, which leads to abnormal processing and presentation of self antigens. Superantigens are considered to be one of the most effective bacterial components to induce inflammatory reactions and to take part in the development and course of autoimmune mechanisms. It has long been known that defects in the host defense mechanism render the individual susceptible to infections caused by certain microorganisms. Impaired exclusion of microbial antigens can lead to chronic immunological activation which can affect the tolerance to self components. Defects in certain components of the immune system are associated with a higher risk of a development of autoimmune disease. The use of animal models for the studies of human diseases with immunological pathogenesis has provided new insights into the influence of immunoregulatory factors and the lymphocyte subsets involved in the development of disease. One of the most striking conclusion arising from work with genetically engineered immunodeficient mouse models is the existence of a high level of redundancy of the components of the immune system. However, when genes encoding molecules involved in T cell immunoregulatory functions are deleted, spontaneous chronic inflammation of the gut mucosa (similar to human inflammatory bowel disease) develops. Surprisingly, when such immunocompromised animals were placed into germfree environment, intestinal inflammation did not develop. Impairment of the mucosal immune response to the normal bacterial flora has been proposed to play a crucial role in the pathogenesis of chronic intestinal inflammation. The use of immunodeficient models colonized with defined microflora for the analysis of immune reactivity will shed light on the mode of action of different immunologically important molecules responsible for the delicate balance between luminal commensals, nonspecific and specific components of the mucosal immune system. 相似文献
10.
Abstract: Numerical modelling was used to follow the moisture movement and strain in a composite system – an unrestrained, single wood panel coated with a layer of gesso, in response to cyclic sinusoidal variations in relative humidity (RH). The allowable magnitude of the variations, below which physical damage of the gesso layer on the wood does not occur over a selected time of exposure, was derived as a function of cycle duration, panel thickness and moisture diffusion configuration. The dimensional response of wood substrate becomes subject to restraint by the applied layer of gesso. The panels do not respond significantly to diurnal fluctuations or shorter irrespectively of the panel thickness. The panels respond more and more significantly when the duration of the fluctuations increases until a certain critical period at which the panel fully responds to each cycle. The analysis of the data obtained indicates that moderate RH variations within the approximate range 50 ± 15% are safe. This safe range was derived using the extremes of conservative criteria of the gesso’s fatigue fracture and assumption of worst‐case wooden substrate response. The reduction of allowable amplitude of RH cycles because of decrease in the gesso’s modulus of elasticity and thickness is discussed. 相似文献