Physical Properties of Soy Bean and Broad Bean 11S Globulin Gels Formed by Transglutaminase Reaction

Physical properties of transglutaminase-induced glycinin and legumin gels were compared with thermally induced gels. Results of deformation tests showed that transglutaminase-induced gels were more rigid and elastic than thermally induced gels. From creep compliance tests, all elastic moduli and viscosities except Newtonian viscosity were higher for transglutaminase-induced gels. Electron micrographs revealed that network structures of transglutaminase-induced gels were composed of larger unit particles forming more developed strands and clusters. More rigid and elastic gels were formed from glycinin as compared to legumin by both gelling methods.     

Removal of carbon contamination in ETEM by introducing Ar during electron beam irradiation

We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 has absorption at ultraviolet wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film of an electron-beam excitation-assisted (EXA) optical microscope. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal. The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA optical microscope is useful for analysis of growth of a crystal, bioimaging and so on.     

Thermal Gelation Mechanism of Legumin from Broad Beans

The legumin formed a gel through formation of soluble aggregates and their juncture. Electron microscope studies indicated the legumin molecules associated to form strands. These strands then formed network-like structures, finally resulting in gel formation. The thickness of those strands and network constituents was irregular (8.3 to 36 nm). A presumed process of thermal legumin gelation was hypothesized. A comparison of gelation behaviors in legumin and in glycinin was also studied.     

电子束辐照处理烟道气：CO对脱出NOx和SO2的影响

一、引言用电子束辐照来脱出烟道气中NO_x和SO_2的思想是1971年由日本原子能研究所和日本荏原公司共同提出并进行研究的。1974年荏原公司建立了处理燃烧重油所产生的烟道气的第一座中试厂。在这座中试厂的实验基础上又在日本钢铁公司建立了更大规模的中试厂     

M-Si-B合金非晶形成能力的CALPHAD模式评估

Evaluation of the amorphous-forming ability of M-Si-B ternary alloys using CALPHAD approach@長谷部光弘$日本九州工业大学 @OHTANI Hiroshi$Department of Materials Science and Engineering, Kyushu Institute of Technology @HASEBE Mitsuhiro$Department of Materials Science and Engineering, Kyushu Institute of Technology…     

TEXTURAL PROPERTIES OF STARCH GELS FILLED WITH COLLAGEN AND CHITIN

Chitin and collagen powders were added as fillers to various starches (potato, sweet potato, kudzu, corn, wheat, and mung bean), heated at 100C for 10 min with stirring to make a hot paste and allowed to gel quiescently at 5C for 2 h in order to investigate the effects of the filler particle on the texture of the gels. The parameters of hardness, cohesiveness and adhesiveness were determined instrumentally and compared. The filler particles of collagen and chitin were effective in increasing hardness of corn starch gels but had no effects on hardness of the potato, sweet potato, kudzu, wheat and mung bean starch gels except at the lower starch concentrations. Cohesiveness was increased slightly in all starch gels by addition of collagen whereas chitin addition only increased the cohesiveness of sweet potato and mung bean starch gels. Collagen and chitin addition increased adhesiveness all of starch gels, but the intensity of the effect was most remarkable for collagen. The observed differences in effects was apparently due to differences in the interactions between starches and filler particles arising from differences in the surface properties of the filler particles.     

Molecular Forces in Thermal Association-Dissociation and Gelation of Legumin from Broad Beans

A 0.5% legumin solution when heated showed neither dissociation into constituent subunits of the legumin nor gel formation, but association of the legumin molecules to form soluble aggregates and network-like structures occurred. Sulfhydryl content of the protein were shown to possibly be responsible for the depression of dissociation. In the 12.5% legumin solution, gelling process and gel properties were affected by reagents such as NaSCN, N-ethylmaleimide and 2-mercaptoethanol. Results indicated that hydrophobic interactions and disulfide bonding helped to enhance the gelling process, where contributions of those forces to the rate of soluble aggregate formation and their junctures were notable.     

Corrosion of Silicon Nitride Ceramics in Aqueous Hydrogen Chloride Solutions

The leaching behavior of pressureless sintered and hotpressed Si₃N₄ containing Y₂O₃, Al₂O₃, and AlN and hot isostatically pressed Si₃N₄ without additives was studied in 0.1 M to 10 M HCl aqueous solutions at 50° to 100°C. Y and Al ions contained in the grain-boundary phase dissolved in HCl solutions, but dissolution of the Si ion from the matrix was negligible. The dissolution of Y and Al ions in HCl solutions was adequately described by a surface-chemical-reaction-controlled, shrinking-core model in <1 M HCl solutions and by a diffusion-controlled, shrinking-core model in >5 M HCl solutions. The rates of dissolution of both Y and Al ions decreased as the degree of crystallinity of the grain-boundary phase increased. The fracture strength of the corroded samples linearly decreased with increasing degree of dissolution of soluble Y and Al ions.     

Dual-colour microscopy of single fluorophores bound to myosin interacting with fluorescently labelled actin using anti-Stokes fluorescence

We have refined prismless total internal reflection fluorescence microscopy with extremely low background to visualize single fluorophores attached to protein molecules interacting with a filamentous biopolymer labelled with different colour fluorophores. By using Stokes and anti-Stokes fluorescence, two different colour fluorescences from two different colour fluorophores excited with a single wavelength laser can be observed simultaneously. This microscopy was applied to visualize motor proteins, actin and myosin molecules. Single myosin molecules labelled with a tetramethylrhodamine-5-iodoacetamide interacting with a BODIPY FL-labelled actin filament, a filamentous polymer of actin molecules, were observed clearly and simultaneously in aqueous solution. Individual hydrolysis reactions of Cy3-labelled ATP by single myosin molecules and sliding of a BODIPY FL-labelled actin filament along the myosin molecules could also be observed simultaneously. Thus, this technique is useful for observing single molecular processes of proteins interacting with a biological macromolecule such as an actin filament and a DNA.