Novel thermally and mechanically stable hydrogel for enzyme immobilization of penicillin G acylase via covalent technique

κ‐Carrageenan hydrogel crosslinked with protonated polyethyleneimine (PEI⁺) and glutaraldehyde (GA) was prepared and evaluated as a novel biocatalytic support for covalent immobilization of penicillin G acylase (PGA). The method of modification of the carrageenan biopolymer is clearly illustrated using a schematic diagram and was verified by FTIR, elemental analysis, DSC, and INSTRON using the compression mode. Results showed that the gels' mechanical strength was greatly enhanced from 3.9 kg/cm² to 16.8 kg/cm² with an outstanding improvement in the gels thermal stability. It was proven that, the control gels were completely dissolved at 35°C, whereas the modified gels remained intact at 90°C. The DSC thermogram revealed a shift in the endothermic band of water from 62 to 93°C showing more gel‐crosslinking. FTIR revealed the presence of the new functionality, aldehydic carbonyl group, at 1710 cm^?1 for covalent PGA immobilization. PGA was successfully immobilized as a model industrial enzyme retaining 71% of its activity. The enzyme loading increased from 2.2 U/g (control gel) to 10 U/g using the covalent technique. The operational stability showed no loss of activity after 20 cycles. The present support could be a good candidate for the immobilization of industrial enzymes rich in amino groups, especially the thermophilic ones. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Measurement of Inlet Drop Size Distribution of Secondary Dispersions by Laser Particle Size Analyzer

Knowledge of the Oil drop size distribution, in addition to the mean drop size, is necessary in order to characterize secondary dispersions. Furthermore, the capture efficiency for any drop flowing through a packed bed depends upon its Oil inlet drop diameter. The Oil/Water secondary dispersions produced by the centrifugal pump were analyzed at regular intervals during an experiment to ascertain the drop size distribution and to check that the feed to the coalescence bed was consistent. New techniques that were developed for measurement of drop size distribution of secondary dispersions using Laser Particle Size Analyzer, which consisted of (He/Ne) laser emitter and laser receiver and lenses. The Laser Particle Size Analyzer was fixed directly to the experimental equipment by using the special designed circular cell. The measurement of drop size distribution was done by computer system with application software package. The new mean drop diameter (i.e., d₂₁) equation has been derived theoretically and the results that predicted from this equation exhibited a maximum error of ±15% from the experimental data.     

Processing of an East Mediterranean phosphate ore sample by an integrated attrition scrubbing/classification scheme (part one)

ABSTRACT

In this study, an Egyptian siliceous/calcareous sedimentary phosphorite was subjected to beneficiation by unsophisticated economic means. The mineralogical and chemical examination showed that the ore sample is a typical example for the East Mediterranean phosphorites with 21.80% P₂O₅, 10.93% acid insoluble and 16.61% loss on ignition. The primary crushed sample was classified into three size fractions without any milling and they were separately subjected to an integrated attrition scrubbing study. Screening and classification on the 2.30 mm and 0.071 mm sieves were sequentially introduced with the attrition process. The effect of solid%, attrition speed and time were studied and optimized for each size fraction. Results showed that three products were produced and could be categorized according to their P₂O₅ content into the coarse tailing (+2.30 mm), the attrition concentrate (?2.30 + 0.071 mm) and the phosphate fines (?0.071 mm). The P₂O₅ contents of these products reached 12.24%, 28.72% and 18.77%, with P₂O₅ distribution reached 6.74%, 51.75% and 41.51%, respectively. A global P₂O₅ of 28.72% was obtained with a recovery of about 52%. A tentative flow-sheet for the whole process was postulated.     

Effects of refining on chemical and physical properties of palm oil products

Some chemical changes in the composition and physical properties of palm oil products are discussed. The effects of bleaching and deodorization on oxidative properties and possible isomerization and interesterification of the fatty acids were indicated from laboratory refining experiments. Investigation of commercial samples of refined palm oil products showed that the conjugated dienes and trienes formed are minimal, indicating the use of good quality raw materials and mild processing conditions. Very little isomerization occurred in commercial refined products as indicated from the level oftrans acids, and changes in the POP to PPO triglycerides due to possible interesterification were insignificant. Changes in physical properties were inevitable due to the removal of free fatty acids and diglycerides and to minor impurities.     

Cancellation of bulging effect on mould level in continuous casting: Experimental validation

During the continuous casting of steel, several disturbances occur and affect all the parts of the caster, including the mould where the molten steel has to be stable for good quality of the final product. Especially at high casting velocities, the bulging generates important level fluctuations which cannot be efficiently rejected by the classical controllers. To address these problems, this paper proposes an architecture combining disturbances estimation and compensation techniques. It operates as an additional module and does not need any change of the main controller currently implemented. The entire structure has been tested successfully through several simulations and by means of intensive water model experiments.     

A predictive bottom-up parser

Despite all advances in parsing, parser size, conflict resolution and error recovery are still of important consideration. In this research, we propose a predictive bottom-up parser. The parser is implemented in two versions. Both versions constitute an algorithm that simulates the run of a shift–reduce automaton, defined and constructed in a way that integrates its parsing actions with reduction prediction, conflict resolution and error recovery. However, the first implementation version performs explicit shift–reduce parsing actions based on implicit prediction of the reduction sequences. The second one performs parsing actions based on explicit prediction of the reduction sequences with implied shift–reduce actions. The proposed parser has been experimented against the ones based on similar approaches. 10–20% reduction of the parser size has been achieved, with a parsing behaviour proportional to a factor reflecting the grammar ambiguity.     

Nondegradative additive manufacturing of medical grade copolyesters of high molecular weight and with varied elastic response

Although additive manufacturing through melt extrusion has become increasingly popular as a route to design scaffolds with complex geometries the technique if often limited by the reduction in molecular weight and the viscoelastic response when degradable aliphatic polyesters of high molecular weight are used. Here we use a melt extruder and fused filament fabrication printer to produce a reliable nondegradative route for scaffold fabrication of medical grade copolymers of L-lactide, poly(ε-caprolactone-co-L-lactide), and poly(L-lactide-co-trimethylene carbonate). We show that degradation is avoided using filament extrusion and fused filament fabrication if the process parameters are deliberately chosen based upon the rheological behavior, mechanical properties, and polymer composition. Structural, mechanical, and thermal properties were assessed throughout the process to obtain comprehension of the relationship between the rheological properties and the behavior of the medical grade copolymers in the extruder and printer. Scaffolds with a controlled architecture were achieved using high-molecular-weight polyesters exhibiting a large range in the elastic response causing negligible degradation of the polymers. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48550.     

Perception of temporally filtered X-ray fluoroscopy images

For noisy X-ray fluoroscopy image sequences we quantitatively evaluated image quality after digital temporal filtering to reduce noise. Using an experimental paradigm called a reference/test adaptive forced-choice method we compared detectability of stationary low-contrast disks in filtered and unfiltered, computer-generated image sequences. In the first experiment, a low-pass first-order recursive filter used in X-ray fluoroscopy was found to be much less effective at enhancing detectability than predicted from the reduction of display noise variance, a common measurement of filter effectiveness. Detectability was reasonably predicted by a nonprewhitening human-observer model (NPW-HVS) that included an independently determined human temporal-contrast-sensitivity function. In another experiment, designed to test models over a range of temporal frequencies, we used paired high-pass and low-pass temporal filters that both reduced noise variance by 25%. The high-pass filter was artificially applied to the noise only and greatly improved detectability, while the low-pass filter had little effect. The human-observer model quantitatively described the measurements, but classical prewhitening and nonprewhitening signal detectors did not. As compared to the nonprewhitening, spatio-temporal matched filter, human-observer efficiency was low and variable at 2.1%, 2.9%, and 0.06% for 60 frames of unfiltered low-pass and high-pass noise, respectively. As compared to this detector, humans were not very effective at combining information across frames. On the other hand, signal to noise ratios (SNR's) from the human-observer model were comparable to human performance, and efficiencies were reasonably constant at 40%, 52%, and 32%, respectively. We conclude that it is imperative to include human-observer models and experiments in the analysis of noise-reduction filtering of noisy image sequences, such as X-ray fluoroscopy.     

Detection improvement in spatially filtered x-ray fluoroscopy image sequences

The effect of spatial noise-reduction filtering on human observer detection of stationary cylinders mimicking arteries, catheters, and guide wires in x-ray fluoroscopy was investigated in both single image frames and image sequences. Ideal edge-preserving spatial filtering was simulated by filtering of the noise before addition of the target cylinder. This allowed us to separate the effect of edge blurring from those of noise reduction and spatial noise correlation. We used three different center-weighted averagers that reduced pixel noise variance by factors of 0.75, 0.50, and 0.25. As compared with no filtering, the effect of filtering on detection in single images was statistically insignificant. This indicated an adverse effect of spatial noise correlation on detection that countered the effect of noise reduction. By comparison, spatial filtering significantly improved detection in image sequences and yielded potential x-ray dose savings of 26-34%. Comparison of results with two observer models suggested that human observers have an improved detection efficiency in spatially filtered image sequences as compared with white-noise sequences. Pixel noise reduction, a measure commonly used to assess filter performance, overestimated the effect of filtering on detection and was not a good indicator of image quality. We conclude that edge-preserving spatial filtering is more effective in sequences than in single images and that such filtering can be used to improve image quality in noisy image sequences such as x-ray fluoroscopy.     

Compact broadband planar orthomode transducer

The design and test results of a compact C-band orthomode transducer are presented. The transducer comprises four rectangular probes orthogonally arranged in a circular waveguide, designed to work in the WG13 band. Measurements of the system in the frequency range 4.64 - 7.05 GHz agree very well with simulation results and show a cross-polarisation level below -58 dB, a return loss of about -20 dB, and an insertion loss difference of less than 0.18 dB between the orthogonal polarisation modes across the full waveguide band.