Photocatalysts using hexa- and octa-titanates with different tunnel space for water decomposition

Photocatalytic activities for water decomposition were examined for photocatalysts using hexa- and octa-titanates and TiO2(B) with different tunnel space in the structure. Using RuO2 as promoter, M2Ti6O13 (M = Li, Na, K, Rb) showed the stoichiometric production of Hz and O₂ except for Li, whereas H₂Ti₈O₁₇ and TiO₂(B) had very low activity producing only hydrogen as a product. The effects of promoters on Na₂Ti₆O₁₃ showed that the activity increased in the order of RuO₂ > RuO₂ + IrO₂ > IrO₂ > RuO₂ + Pt > MnO₂. These effects along with other related ones are discussed: it emerges that the presence of the tunnels is important for the achievement of high photocatalytic activity.     

Motion analysis of a tripod parallel mechanism

A tripod parallel mechanism consists of three links of fixed length and a rigid platform, and these are connected by revolute joints. The platform can achieve sixdegrees-of-freedom (6-DOF) motion by the coordinated movement of the bottom ends of the three links on a horizontal plane. This mechanism has advantages over the more common six extendible parallel manipulators. It has a much larger work space and a simple structure. In this article, we show that the vector analysis for this tripod parallel mechanism and the derivation of the positions of the three bottom ends of the links in an arbitrary attitude of platform can be found by inverse kinematics and the conditions of geometrical constraint. Then, by a numerical simulation, the trajectories of the bottom ends of the three links are shown.     

Effects of dressing containing plant sterol on serum cholesterol concentration and the safety evaluation in borderline or mildly hypercholesterolemic Japanese subjects

In a placebo-controlled double-blind study, we examined the effects of dressing containing plant sterol (PS) on blood lipids and the safety in Japanese borderline or mildly hypercholesterolemic subjects. Fifty-nine subjects [total cholesterol (TC) concentration > or = 200 mg/dL] were randomly divided into two groups and were given daily 15 g of dressing containing 800 mg of PS [PS(+)-group] or without PS [PS(-)-group] for 12 weeks. Every 4 weeks, fasting blood was examined and subjective symptoms were analyzed. Serum TC, low-density lipoprotein cholesterol (LDL-C) and apolipoprotein B (ApoB) concentrations did not change in the PS(-)-group, while TC and ApoB significantly decreased in the PS(+)-group at 8 and 12 weeks and LDL-C at 4, 8 and 12 weeks. Moreover, serum TC, LDL-C and ApoB concentrations were significantly lower than those of PS(-)-group at 8 and 12 weeks. Other laboratory tests were all in normal ranges and no adverse events were observed. The results indicated that PS-containing dressing decreased serum TC, LDL-C and ApoB concentrations in borderline or mildly hypercholesterolemic subjects. It is therefore proved that the dressing containing PS is helpful in maintaining blood cholesterol level normal and hence, the health of Japanese.     

MgATP-induced conformational changes in a single myosin molecule observed by atomic force microscopy: periodicity of substructures in myosin rods

This paper discusses the conformational changes in a single myosin molecule directly observed using atomic force microscopy (AFM). The myosin molecules were pretreated in rigor solutions without MgATP or in relaxed solutions with various concentrations of MgATP. The images of these molecules were obtained using a tapping mode AFM. The results indicate that the orientation of the myosin's heads and tail strongly depend on the MgATP concentration. Without using MgATP, almost all of the myosin molecules are in the extended form; however, when MgATP is used, the molecules bend according to the level of MgATP concentration. The mean-square end-to-end distance of the myosin molecules is significantly shorter with p[MgATP] = 4 than with p[MgATP] = 6. The rod region did not show the same level of intensity along their length in the extended form. The rods exhibited clusters of discontinuity, which were identified as substructures. The size of these substructures change at intervals that are multiples of 14.3-14.5 nm, which reflects the periodicity of the alpha-helical coiled coils. The substructure clusters also correspond to the myosin crossbridge spacing in muscles (14.3 or 43 nm). These results suggest that the myosin's head bends in conjunction with the bending or tilting in the helical substructures. Conformational changes of the myosin molecule induced by MgATP seem to mimic the molecular motions in a muscle's force generation process.     

Computer-aided design of a factor Xa inhibitor by using MCSS functionality maps and a CAVEAT linker search

We have investigated a new approach to efficiently find a novel inhibitor against a serine protease (i.e. an activated coagulation factor X, FXa) by using de novo design programs and the X-ray crystal structure of the target enzyme. FXa is a coagulant enzyme that generates thrombin (a serine protease) and participates in both intrinsic and extrinsic coagulation pathways. We adopted multiple copy simultaneous search (MCSS) and CAVEAT linker search techniques, which disclosed a novel FXa inhibitor (T01312) consisting of two binding moieties (the benzamidinyl and adamantyl groups) and a linker unit (the carboxybenzylamine group). The inhibitory activity of T01312 against FXa was determined to be a small K(i)-value of 48nM, which is two orders of magnitude smaller than that against thrombin. An X-ray crystal analysis of T01312 complexed with trypsin (an analogue of FXa) and docking studies of T01312 with trypsin and FXa showed that: (i) the benzamidinyl group is a predominant binding moiety in the anionic pocket (S1 site) with an asparatic acid residue; (ii) a hydrophobic pocket (S4 site) is the binding site of the adamantyl group; (iii) the carboxylate group of the linker contributes to the selectivity for FXa against thrombin. Thus, the combination of the knowledge of the X-ray crystal structure of the target molecule with MCSS and CAVEAT linker search techniques proved to be an effective hit-finding method that does not require the screening of huge compound libraries.     

Critical heat flux of binary mixtures in pool boiling and its correlation in terms of Marangoni number

Critical heat flux (CHF) in nucleate pool boiling of binary mixtures was newly measured with a horizontal platinum wire, 0.5 mm in diameter, and heated by DC, over the full range of concentrations. Seven mixtures were selected with the intent to cover various types of mixtures: methanol/water, ethanol/water, methanol/ethanol, ethanol/n-butanol, methanol/benzene, benzene/n-heptane and water/ethylene glycol, each in the saturated state at atmospheric pressure. Total 311 raw CHF data were obtained at 75 concentrations including pure components.Aqueous mixtures of methanol and ethanol revealed significant increase of CHF compared to either CHF linearly interpolated between pure components or CHF predicted from a single component correlation with use of the mixture properties. Three organic mixtures showed more or less the same level as an interpolated CHF, while the remaining two mixtures of methanol/benzene and water/ethylene glycol gave the reduced CHF by 20% and 50% at most, respectively. Marangoni number was introduced as a controlling variable to explain the observed increased, invariable, or reduced CHF, and an empirical correlation was developed.     

Curative laparoscopic surgery for early gastric cancer: five years experience

Sixty-one patients who were diagnosed with mucosal gastric cancer have been successfully treated with two laparoscopic techniques at our institute from March 1992 to March 1997. One is laparoscopic wedge resection of the stomach using a lesion-lifting method for lesions of the anterior wall, the lesser curvature, and the greater curvature of the stomach. The other is laparoscopic intragastric mucosal resection for lesions of the posterior wall of the stomach and near the cardia or the pylorus. Indications are as follows: (1) preoperatively diagnosed mucosal cancer; (2) <25 mm diameter elevated lesions; and (3) <15 mm diameter depressed lesions without ulcer formation. Patients were discharged in 4 to 8 days uneventfully. There was no major complication or mortality. The resected specimens had sufficient surgical margins horizontally (16 +/- 5 and 8 +/- 4 mm, respectively) and vertically. In one patient histologic examination revealed slight tumor infiltration into the submucosal layer with lymphatic invasion. He underwent gastrectomy with lymph node dissection 1 month after surgery. Otherwise, histologic examination revealed curative surgery. All patients in the series have survived during the 4- to 65-month follow-up period. There have been two recurrences in the series, both of which were found near the staple line 2 years after the initial surgery and were still mucosal lesions. They were successfully treated by open gastrectomy and laser irradiation. A separate early gastric cancer was found 2 years after the initial surgery in one patient, who then underwent curative open gastrectomy. In conclusion, if the patients are selected properly, these laparoscopic procedures are curative, minimally invasive treatment for early gastric cancer.     

Characteristics of the Mach Disk in the Underexpanded Jet in which the Back Pressure Continuously Changes with Time

When the high-pressure gas is exhausted to the vacuum chamber from the nozzle, the underexpanded supersonic jet contained with the Mach disk is generally formed. The eventual purpose of this study is to clarify the unsteady phenomenon of the underexpanded free jet when the back pressure continuously changes with time. The characteristic of the Mach disk has been clarified in consideration of the diameter and position of it by the numerical analysis in this paper. The sonic jet of the exit Mach number Me=1 is assumed and the axisymmetric conservational equation is solved by the TVD method in the numerical calculation. The diameter and position of the Mach disk differs with the results of a steady jet and the influence on the continuously changing of the back pressure is evidenced from the comparison with the case of steady supersonic jet.     

Estimation of daily inhalation rate in preschool children using a tri-axial accelerometer: a pilot study

The activity of 5- to 6-year-old Japanese children (n = 29) was monitored for 3 consecutive days, including one weekend day, using an ActivTracer tri-axial accelerometer. The daily inhalation rate and time spent in sedentary, light, or moderate to vigorous levels of physical activity (MVPA) were estimated from the accelerometer measurements based on previously developed regression equations. The 3-day mean daily inhalation rate (STPD) was estimated at 8.3 ± 1.4 m³ day⁻¹ in 10 subjects who completed 3 days of monitoring. The time spent in sedentary, light, or MVPA each day was 320, 415, and 81 min day⁻¹, respectively. Analysis of between-day reliability indicated that 3 days of monitoring with the ActivTracer tri-axial accelerometer provided an acceptable estimate of daily inhalation rate (intra-class correlation coefficient [ICC] = 0.892), but low to moderate reliability for the time spent in different levels of activities (ICC = 0.43 to 0.58). We observed a significant difference in the daily inhalation rate between weekdays and the weekend day, possibly due to differences in time spent in MVPA. This finding suggests that a weekend day should be included to obtain more reliable estimates of daily inhalation rate using an accelerometer.