Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer

Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases.     

Liquid Marble Patchwork on Super-Repellent Surface

Liquid marble (LM) is a droplet that is wrapped by hydrophobic solid particles, which behave as a non-wetting soft solid. Based on these properties, LM can be applied in fluidics and soft device applications. A wide variety of functional particles have been synthesized to form functional LMs. However, the formation of multifunctional LMs by integrating several types of functional particles is challenging. Here, a general strategy for the flexible patterning of functional particles on droplet surfaces in a patchwork-like design is reported. It is shown that LMs can switch their macroscopic behavior between a stable and active state on super-repellent surfaces in situ by jamming/unjamming the surface particles. Active LMs hydrostatically coalesce to form a self-sorted particle pattern on the droplet surface. With the support of LM handling robotics, on-demand cyclic activation–manipulation–coalescence–stabilization protocols by LMs with different sizes and particle types result in the reliable design of multi-faced LMs. Based on this concept, a single bi-functional LM is designed from two mono-functional LMs as an advanced droplet carrier.     

A set expression based inheritance system

This paper describes a new formalism for inheritance systems, based on the formal semantics of set expressions. Using the formalism, it is possible to define new semantic classes by arbitrary set expressions operating on previously defined classes. Thus generalizing bothIS-A links andIS-NOT-A links and adding the set intersection operation. We present an efficient algorithm which follows these definitions to deduce the properties implied by the inheritance network, i.e., the properties of the classes containing a given element. The application which motivated the development of the formalism, namely semantic disambiguation of natural language, is also described.     

Simultaneous over- and underconfidence: The role of error in judgment processes.

Two empirical judgment phenomena appear to contradict each other. In the revision-of-opinion literature, subjective probability (SP) judgments have been analyzed as a function of objective probability (OP) and generally have been found to be conservative, that is, to represent underconfidence. In the calibration literature, analyses of OP (operationalized as relative frequency correct) as a function of SP have led to the opposite conclusion, that judgment is generally overconfident. Reanalysis of 3 studies shows that both results can be obtained from the same set of data, depending on the method of analysis. The simultaneous effects are then generated and factors influencing them are explored by means of a model that instantiates a very general theory of how SP estimates arise from true judgments perturbed by random error. Theoretical and practical implications of the work are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Optimal Control of Solar Sludge Dryers

Optimal control (OC) methodology is used to develop a control policy for a batch-operated solar sludge dryer with ventilation as the dominant control. Batch performance criteria and instantaneous optimization criteria are developed for two economic environments: quota limited and capital limited. The general formulation, as well as simplifications resulting from the multiplicative form of the evaporation (drying) rate model, are presented. The multiplicative form results in a single constant number, called here “control intensity,” which is used to guide the on-line control decisions. These decisions turn out to be independent of the dry solids content (DSC) of the sludge. Further simplification, by assuming a strictly constant weather, is used to demonstrate the general effects of the economic and physical (weather) environment on the solution.

The OC approach is used to address, via simulation under realistic weather conditions, several design, operation, and pricing problems. In particular, the following have been considered: sizing of the ventilation fans, determination of a fair fee for sludge disposal, finding the best final DSC, and evaluating the effect of the price of electricity. Regarding the particular location represented by the data, it has been shown that the installed capacity of the ventilation fans should be increased and that there seems to be sufficient economic incentive for solar drying.     

Immunohistochemical study of epithelioid hemangioendothelioma in the leg: a case report

Epithelioid hemangioendothelioma is a relatively rare lesion. Although its histogenesis has been well described, its immunohistochemical characteristics remain controversial. A case of epithelioid hemangioendothelioma of the soft tissue of the right leg in a 67-year-old Chinese woman is reported. Histologic findings of intracytoplasmic lumina in the tumor cells and positive immunostaining for vimentin, factor VIII-related antigen. CD34 and Ulex europaeus agglutinin 1 (UEA-1) were obtained, demonstrating differentiation of the tumor cells to endothelial cells, although staining for antibodies to cytokeratins AE1/AE3 and CAM5.2 was weak. CD34 as well as Factor VIII-related antigen is a useful marker of endothelial differentiation in this tumor. A review of the literature is also presented.     

安全防护不容一丝轻忽—黑客入侵胰岛素泵的启发

不久前,我刚好有机会参加了一个医疗论坛,会议开幕演讲吸引了我的注意,因为会议上现场示范了如何以黑客手法侵入胰岛素泵。这场会议十分具有启发性,它让我们看到了黑客如何通过无线连接,从远端入侵存在安全漏洞的医疗设备。     

Adsorption of urea,creatinine, and uric acid from three solution types using spherical activated carbon and its recyclability

In this paper, we propose that the urinary toxins from the wastewater be adsorbed on an adsorbent such as spherical activated carbon and the latter be regenerated by subjecting it to high temperatures to recycle activated carbon and also to recycle the water used in dialysis. We studied the adsorption of artificial waste dialysate, which is a mixed solution of urea, creatinine, and uric acid, and the separate solutions for each of these and found that their extents of adsorption onto the spherical activated carbon material were nearly identical. The amount of adsorption was approximately 1.4 mg·g^-1 for urea, 18 mg·g^-1 for creatinine, and 20 mg·g^-1 for uric acid. The urea, creatinine, and uric acid adsorbed onto the spherical activated carbon decomposed on heat treatment at 500℃, and the adsorption capacity of the spherical activated carbon was regenerated. Our study successfully demonstrated that the spherical activated carbon can be recycled in the waste dialysate treatment process.     

A carboxyalkanethiol monolayer modified electrode for blocking of ascorbic acid oxidation and its application to a biosensor

Upon the application of amperometric biosensor to the biological fluid, ascorbic acid interferes the amperometric determination of analytes, because the oxidative potential of ascorbic acid is lower than that of electro active substances such as H₂O₂ produced by the enzymatic reaction. In this study we propose a method to block ascorbic acid based on the electrostatic interaction with self-assembled monolayer (SAM) and its application of the surface modified electrode to biosensor. In order to form SAM on the gold electrode with carboxyl group, 7-carboxy-heptanethiol (7-CHT) was used. The 7-CHT modified electrode did not show anodic response to ascorbic acid, but oxidized phenanthroline cobalt complex [Co(phen)₃²⁺], which can be used as a mediator of biosensor. Thus, the 7CHT-modified electrode was applied to biosensor mediated with Co(phen)₃²⁺. Fructose dehydrogenase (FDH) was immobilized to the 7-CHT modified electrode. Fructose was determined selectively with the FDH/7-CHT modified electrode at the range of 0.2-2 mM.