The effect of the EAAEAE insert on the property of human metallothionein-3

MT3 shows apparently different properties and function fromMT1 even though they have 70% sequence homology. Possibly thetwo inserts, Thr5 and a negatively charged hexapeptide at position-55in MT3, play important roles. A series of MT3 variants aroundthe EAAEAE hexapeptide have been prepared by site-directed mutagenesisand their properties and reactivity towards pH, EDTA and DTNBhave been studied. Our detailed studies revealed that the EAAEAEinsert is essential to the property of MT3. It is the hexapeptideinsert, to some extent, making the MT3     

The influence of Glu44 and Glu56 of cytochrome b5 on the protein structure and interaction with cytochrome c

The gene encoding trypsin-solubilized bovine liver microsomalcytochrome b₅ (82 residues in length) has been mutated, in whichthe codons of Glu44 and Glu56 were changed to those of Ala.The mutated genes were expressed in Escherichia coli successfullyand three mutant proteins (E44A, E56A and E44/56A) were obtained.The UV-visible, CD and ¹H NMR spectra of proteins have beenstudied. The results show that the mutagenesis at surface residuesdoes not alter the secondary and tertiary structures of cytochromeb₅ significantly. The interactions between recombinant cytochromeb₅ and its mutants with cytochrome c were studied by using opticaldifference spectra. The results demonstrated that both Glu44and Glu56 of cytochrome b₅ participate in the formation of acomplex between cytochrome b₅ and cytochrome c.     

3GPP认证与密钥协商协议安全性分析

通用移动通信系统采用3GPP认证与密钥协商协议作为其安全框架,该协议对GSM存在的安全隐患作了有效的改进.对3GPP认证与密钥协商协议进行安全性研究,分析其容易遭受4种类型攻击方式.为了解决上述存在的安全隐患,提出在位置更新与位置不变两种情况下的基于公钥密码学的认证与密钥协商协议,采用形式化的分析方式证明了所提出算法的安全性,并将该协议与已有协议在安全性方面进行了比较.结果显示,所提出的协议算法能够极大地增强3GPP认证与密钥协商协议的安全性.     

国民体质监测系统

随着经济社会的发展,人们不仅追求更高的物质生活,也更加关注体质健康和监测自身体质信息.本文介绍了国民体质监测系统,用于集中监测用户个人信息、健康数据和基础运动数据,为健康管理和慢性病干预提供了完整的一站式服务解决方案.本系统包含基于.NET MVC的数据统计分析子系统、基于Windows服务的数据同步服务与汇总服务以及基于Zabbix的系统监控程序等功能模块,能同时提供Web和移动终端两种访问形式.其中数据统计分析子系统用于统计用户数据,并利用数据挖掘技术给出统计分析结果和科学健身指导方案.数据同步服务用于准备数据,保证系统的数据完整性和一致性.数据汇总服务把省数据中心的地面站数据库数据汇总到中心数据库,中心数据库与数据统计分析子系统进行数据交互.系统监控程序监控服务器联网情况、IIS状态、数据库状态以及系统运行过程中可能发生的错误,并通过短信或邮件给出告警信息.本文设计开发完成的国民体质监测系统,提供了高效的国民体质监测和科学健身指导方案,有利于用户改善自己的身体机能.     

Vague集中的分解定理与相似度量

1 引言目前,在计算机科学及应用的多种领域中,模糊集起着重要的作用。因而其理论及应用都得到了迅速的发展。Vague集从一定意义上讲在对事物属性的描述上较模糊集提供了更多的选择方式,因而已在学术界和工程技术界引起了广泛关注。事实上,Vague集正成为当前模糊信息处理中的一个新兴课题。当前相对于应用而言,Vague集的理论研究方面有待     

一种新的模糊补偿多类支持向量机

支持向量机是Vapnik等学者在统计学习理论的基础上提出的一种新的机器学习方法。针对支持向量机理论中的多类分类问题和对于噪音数据的敏感性,本文提出了一种模糊补偿多类支持向量机算法FC-SVM。该算法是在Weston等人提出的多类SVM分类器的直接构造方法中引入模糊补偿函数,针对每个输入数据对分类结果的两方面影响,将目标函数中的惩罚项不仅进行了模糊化,而且对于分类情况进行了加权补偿,并重构了优化问题及其约束条件,然后重构了Lagrange公式,给出了理论推导。在充分的数值实验基础上,将文中提出的方法应用于建设银行个人房贷的信用评估系统中,得到了较好的实验结果。     

碳纤维布加固钢筋混凝土短柱抗震性能的有限元分析

针对碳纤维布加固钢筋混凝土短柱的破坏特征．建立了适用于反复荷载作用下钢筋混凝土材料的本构关系。考虑材料的非线性、钢筋与混凝土之间的粘结作用、碳纤维布与混凝土之间相对滑移的影响，采用APDL参数化程序设计语言编制的分析程序．分析了碳纤维布加固钢筋混凝土短柱的屈服荷载、极限荷载、破坏荷载及其相应的顶点位移．计算与试验结果基本一致。     

集约度与粗放度的灰色测度公式及其应用

以灰色系统的ＧＭ（１，Ｎ）为基础，推导出集约度与粗放度的灰色测试公式。实际应用表明，这种建立在ＧＭ（１，Ｎ）基础上的集约度与粗放度的灰测度公式，有明显的优点。     

Numerical derivation of pressure–impulse diagrams for prediction of RC column damage to blast loads

Pressure–impulse (P–I) diagrams are commonly used in the preliminary design or assessment of protective structures to establish safe response limits for given blast-loading scenarios. Current practice in generating the pressure–impulse diagram for structure components is primarily based on the simplified single degree of freedom (SDOF) model. The damage criterion is usually defined in terms of deformation or displacement response. Under blast loads, structures usually respond at their local modes, the equivalent SDOF system derived using the fundamental structure response mode might not be suitable. Moreover, structure is often damaged owing to brittle shear failure. In this case, the deformation-based damage criterion might not be able to give an accurate indication of local damage of a structural component. In this paper, a new damage criterion for RC column is defined based on the residual axial load-carrying capacity. A numerical method to generate pressure–impulse diagram for RC column is proposed. Parametric studies are carried out to investigate the effects of column dimension, concrete strength, longitudinal and transverse reinforcement ratio on the pressure–impulse diagram. Based on the numerical results, analytical formulae to predict the pressure–impulse diagram for RC column are derived. A case study shows that the proposed analytical formulae can be easily used to generate pressure–impulse diagram for RC columns accurately. The results are also compared with those obtained from the SDOF approach. It is shown that the proposed method gives better prediction of pressure–impulse diagram than the SDOF approach.     

Nickel-Catalyzed Reductive Coupling of Vinyl Triflates and 1,4-Dicyanobenzene to Access Alkenyl Nitriles

We report a vinyl cyanation reaction of vinyl triflates and readily available 1,4-dicyanobenzene through a nickel-catalyzed reductive coupling process. The reaction is operated under mild conditions with remarkable functional-group compatibility. Cyclic vinyl triflates with various ring sizes and substituents at different positions all reacted smoothly. The synthetic utility is demonstrated by the derivatization of pharmaceutical and natural compounds, a scale-up synthesis, and various functional group transformations. Preliminary mechanistic studies demonstrate that vinyl triflates are more reactive than 1,4-dicyanobenzene towards the oxidative addition to nickel catalyst.