High level expression of recombinant human tumour necrosis factor β (rh TNF-β) in Escherichia coli results in the formation of two portions of protein, namely soluble active protein and insoluble protein which is inactive and aggregates in the form of inclusion bodies (IBs). In this study, a procedure for purification and renaturation of rh TNF-β from inclusion bodies has been designed and verified experimentally with a product purity of more than 90% and a recovery of about 30%. The procedure includes washing of IBs with specific wash buffer (Triton X-100/EDTA/lysozyme/PMSF), their solubilization with 8 mol dm?3 alkaline urea, purification with ion-exchange columns, refolding with renaturation buffer and finally concentration and desalination with an ultrafiltration membrane. The characteristics of the renatured protein were identical with those of purified protein from the soluble fraction as demonstrated by (1) SDS-PAGE, (2) cytotoxic activity on mouse L929 cells, (3) N-terminal amino acid sequence, and (4) gel filtration chromatography. 相似文献
A transport equation for the mean flux in spatially random media is derived, and is referred to as Modified-Levermore–Pomraning equation (M-L–P). It differs from the conventional L–P equations in that |μ| in the latter is replaced by μ in M-L–P. It is shown that when scattering is present the L–P equations are always incorrect in the sense there is not any special situation in which they can lead to an exact result. In particular they always predict the relaxation lengths of the spatial modes incorrectly. On the other hand, the M-L–P equations are exact when the flux at the origin is deterministic, as in some special cases such as half-infinite medium, and infinite medium with a localized source at the origin, when the density of the medium is spatially random. However, the M-L–P equations become approximate when the medium is a finite slab because of the right boundary condition. But the relaxation rates of the spatial modes are always calculated exactly even in finite slab. The nature of approximation inherent in the M-L–P is elucidated by comparison with the exact “stochastic transition matrix formalism” developed earlier in two-stream transport. 相似文献
Hydrogels are polymeric materials widely used in medicine due to their similarity with the biological components of the body. Hydrogels are biocompatible materials that have the potential to promote cell proliferation and tissue support because of their hydrophilic nature, porous structure, and elastic mechanical properties. In this work, we demonstrate the microwave-assisted synthesis of three molecular weight varieties of poly(ethylene glycol) dimethacrylate (PEGDMA) with different mechanical and thermal properties and the rapid photo of them using 1-hydroxy-cyclohexyl-phenyl-ketone (Irgacure 184) as UV photoinitiator. The effects of the poly(ethylene glycol) molecular weight and degree of acrylation on swelling, mechanical, and rheological properties of hydrogels were investigated. The biodegradability of the PEGDMA hydrogels, as well as the ability to grow and proliferate cells, was examined for its viability as a scaffold in tissue engineering. Altogether, the biomaterial hydrogel properties open the way for applications in the field of regenerative medicine for functional scaffolds and tissues. 相似文献
In order to improve the life quality of amputees, providing approximate manipulation ability of a human hand to that of a
prosthetic hand is considered by many researchers. In this study, a biomechanical model of the index finger of the human hand
is developed based on the human anatomy. Since the activation of finger bones are carried out by tendons, a tendon configuration
of the index finger is introduced and used in the model to imitate the human hand characteristics and functionality. Then,
fuzzy sliding mode control where the slope of the sliding surface is tuned by a fuzzy logic unit is proposed and applied to
have the finger model to follow a certain trajectory. The trajectory of the finger model, which mimics the motion characteristics
of the human hand, is pre-determined from the camera images of a real hand during closing and opening motion. Also, in order
to check the robust behaviour of the controller, an unexpected joint friction is induced on the prosthetic finger on its way.
Finally, the resultant prosthetic finger motion and the tendon forces produced are given and results are discussed. 相似文献
The prediction of chronic diseases and their comorbidities is an essential task in healthcare, aiming to predict patients’ future disease risk based on their previous medical records. The accumulation of administrative data has laid a solid foundation for applying deep learning approaches in healthcare. Existing studies focused on the patients’ characteristics such as gender, age and location to predict the risk of the different diseases. However, there are high dimensional, incomplete and noisy problems in the administrative data. In this research, using administrative health data, we implemented graph theory and content-based recommender system approaches to analyse and predict chronic diseases and their comorbidities. Firstly, we used bipartite graphs to represent the relationships between patients and diseases. Then, we projected this graph to a one-mode graph, namely ‘disease network’. After that, six recommender system models with patient features and network features were trained. The outputs of these models are the severity levels of diseases and the predicted diseases with rank. Finally, we evaluated the performance of these models against the same models without network features. The results demonstrated that the models with network features have lower prediction error and better performances for predicting chronic diseases and their latent comorbidities on large administrative data. Among these models, the graph convolution matrix completion model reveals the least amount of error and the best performance for prediction. Further, using a case study of a specific patient, we demonstrated the application of these models in predictive disease risk analysis. Thus, this study showed the potential application of the recommender system approaches to the health sector utilising administrative claim data, which could significantly contribute to healthcare services and stakeholders.
This study proposed a new royal crown-shaped polarisation insensitive double negative triple band microwave range electromagnetic metamaterial absorber (MA). The primary purpose of this study is to utilise the exotic characteristics of this perfect metamaterial absorber (PMA) for microwave wireless communications. The fundamental unit cell of the proposed MA consists of two pentagonal-shaped resonators and two inverse C-shaped metallic components surrounded by a split ring resonator (SRR). The bottom thin copper deposit and upper metallic resonator surface are disjoined by an FR-4 dielectric substrate with 1.6 mm thickness. The CST MW studio, a high-frequency electromagnetic simulator has been deployed for numerical simulation of the unit cell in the frequency range of 4 to 14 GHz. In the TE mode, the offered MA structure demonstrated three different absorption peaks at 6.85 GHz (C-band), 8.87 GHz (X-band), and 12.03 GHz (Ku-band), with 96.82%, 99.24%, and 99.43% absorptivity, respectively. The electric field, magnetic field, and surface current distribution were analysed using Maxwell’s-Curl equations, whereas the angle sensitivity was investigated to comprehend the absorption mechanism of the proposed absorber. The numerical results were verified using the Ansys HFSS (high-frequency structure simulator) and ADS (advanced design system) for equivalent circuit models. Moreover, the proposed MA is polarisation and incident angle independent. Hence, the application of this MA can be extended to a great extent, including airborne radar applications, defence, and stealth-coating technology. 相似文献
A computer-based spatial-filtering velocimeter to measure the surface velocity of natural debris flow is described. This is a simple and interesting technique implemented with a spatial filter constructed as a software program that processes the video image of debris flow instead of a hardware implementation. The surface velocity of the debris flow at the Mt. Yakedake Volcano, Japan, was estimated by this computer-based spatial-filtering method, and the results were compared with those obtained by a hardware-based spatial-filtering method. Computer-based spatial filtering has the important advantage of a capability for tuning the spatial-filter parameters to the target flow. 相似文献
Vic-dioximes, a class of organic chemical compounds, are proposed and characterized for the first time as sensitive materials for volatile organic compound sensing with sorption based chemical gas sensors. Their peculiar sensing properties described in this work originate in the oxime functional group which is a powerful H bond donor interacting strongly but reversibly with H bond acceptors. These specific interactions result in a high preferential enrichment of analyte molecules with H bonding acceptor capabilities in the sensitive material. Accordingly, sensitivity and selectivity for these compounds of vic-dioxime based sensors are high. The advantageous sensing properties are demonstrated in this work with quartz crystal microbalance sensors using 11 selected volatile organic compounds and a set of vic-dioximes varied in their substituents. Vic-dioximes with short alkylthiol substituents were found highly sensitive to such H bond acceptors as organic amines, alcohols, and esters with partition coefficients up to 26,000. At the same time they showed low affinity for aromatic compounds and chlorocarbons. Vic-dioximes are considered powerful sensing materials and interesting for practical use in chemical gas sensor arrays. 相似文献