排序方式: 共有13条查询结果,搜索用时 15 毫秒
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将3-羟基-4′-N,N-二甲氨酸黄酮甲基丙烯酸酯与甲基丙烯酸甲酯和丙烯酸丁酯共聚,制备了量种含有荧光基团的高聚物(PFMB)。基于Fe(Ⅲ)与C1^-形成的配合物对PFMB光极膜荧光的猝灭作用,研制了一种荧光光经行传感器。该传感器用于0.1 ̄6.0mol·L^-1盐酸的测量,显示了良好的重现性和可逆性,响应时间和恢复时间均小于40秒。由于荧光基团是共价固定在聚合物上,该传感器还具有较长的使用寿命 相似文献
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The rotigotine in beagle dog plasma was determined by liquid chromatographic-tandem mass spectrometry. The method of HPLC-MS/MS was used, and detection was performed on a tandem spectrometry in multiple reaction monitoring(MRM) mode. The standard curves are linear in the range of 0.025-12.5 μg L-1, and the recovery is 89%-91%, the intra-day and the inter-day precision are less than 5.6% and 6.8%, respectively. The low limit of quantification(LLOQ) is 0.025 μg L-1. The specificity is good and sensitive, so this method is suitable for the parmacokinetical research of rotigotine. 相似文献
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建立液相色谱-串联质谱(LC-MS/MS)法同时测定半夏厚朴汤中厚朴酚与和厚朴酚在大鼠血浆中的浓度。采用Waters XTerra C18色谱柱,以V(乙腈)∶V(0.1 mmol/L乙酸铵)=75∶25的溶液为流动相,流速为0.2 mL/min,进样量5 μL,等度洗脱方式进行分离,检测模式为负离子条件下的选择性反应监测。厚朴酚与和厚朴酚在1~1 000 μg/L浓度范围内线性关系良好,定量下限均为1 μg/L,方法的检测限(S/N=3)为0.1 μg/L。 准确度与精密度结果显示,方法日间、日内变异均小于15%,相对偏差为厚朴酚3.4%~6.6%,和厚朴酚0.5%~4%。低、中、高3个浓度提取回收率均大于90%。该方法可用于灌胃给予半夏厚朴汤复方水煎液后目标成分厚朴酚与和厚朴酚在大鼠血浆中浓度的同时监测,进而进行半夏厚朴汤中厚朴酚与和厚朴酚在大鼠体内药代动力学研究。 相似文献
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目的: 建立了一种简单、快速、灵敏的高效液相色谱-串联质谱法(LC-MS/MS)测定大鼠血浆中达比加群的浓度,进行了系统的方法学验证研究,并应用于达比加群酯大鼠灌胃给药后的药代动力学研究。方法: 血浆样品经甲醇沉淀蛋白后,通过Ecosil-C18 (150 mm×4.6 mm,5 μm) 色谱柱分离,流动相为甲醇5%甲醇水溶液(含有10 mmol/L甲酸铵及 0.1%甲酸),梯度洗脱。质谱采用电喷雾离子化源正离子条件下多反应监测模式,监测反应离子对为达比加群[M+H]+ m/z 472.2→289.2,内标苯海拉明[M+H]+ m/z 256.1→167.0。结果: 达比加群在 0.25~500 ng/mL 浓度范围内线性关系良好(r =0.9970),定量下限为 0.25 ng/mL;日内、日间精密度(RSD)均小于 9.57%,准确度(RE)均在±6.5%以内;血浆中无内源性物质干扰,基质效应为108%~112%,提取回收率为 92.6%~94.2%;达比加群的血浆样品经室温放置 4 h、-80 ℃三次冻融循环、-80 ℃冻存三周,处理后的样品在进样器内放置 24 h 均稳定;药物浓度超出曲线范围的样品经空白血浆稀释5倍后,其准确度均在±8.00%以内,精密度小于4.92%。结论:本文建立的LC-MS/MS方法学验证,成功适用于大鼠灌胃20 mg/kg给药达比加群酯药代动力学研究。 相似文献
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An high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method for the determination of vinorelbine was presented, and the pharmacokinetics of the aqueous solution and lipid microspheres by intravenous were evaluated. Detection was performed using positive ion electrometry ionization followed by tandem mass spectrometry(ESI-MS/MS). The linear range of the standard curve of vinorelbine is from 1 to 1 000 μg•L-1. The method shows that it is specificity, convenient and suitable for assaying the concentration of vinorelbine in rat plasma, and is used to support pharmacologic studies with vinorelbine and preparation technology for lipid microspheres. 相似文献
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目的:建立LC-MS/MS法测定人血浆中安立生坦浓度并应用于人体药代动力学研究。方法:采用液-液萃取法从人血浆中提取安立生坦和内标后,在Waters Symmetry C18柱(4.6 mm×100 mm, 5 μm)上进行色谱分离,有机相:40%甲醇-60%乙腈,水相:5 mmol/L乙酸铵-10%乙腈,流速:0.5 mL/min,电喷雾离子化源,多反应监测,负离子模式,安利生坦和内标的监测离子对分别为m/z 377.1→301.2和m/z 380.4→301.0。结果:安立生坦在2~2 000 ng/mL浓度范围内线性良好,定量下限为2 ng/mL,批内精密度小于2.72%,批间精密度小于8.98%,经内标归一化后的基质效应在92.3%~98.3%之间,提取回收率在74.6%~80.5%之间。 结论:该方法灵敏、经济、可靠,适用于人血浆中安立生坦浓度的测定,满足人体药代动力学研究。 相似文献
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A rapid, sensitive and selective method for the determination of taxifolin in Beagle dog plasma was described by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Resveratrol was used as internal standard (I.S.). MS detection and quantitation were done by mass spectrometer using selected reaction monitoring at m/z 303 and 285 [M-H]- for taxifolin, and m/z 227 and 185 [M-H]- for resveratrol. The calibration curve is linear (r>0.998) from 2 to 1 000 μg L-1, and the lower limit of quantification(LLOQ) is 2 μg L-1. 相似文献