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采用分子自组装技术制备巯基修饰的基因分子膜修饰金电极,用于竞争式杂交检测黄孢原毛平革菌木素过氧化物酶编码基因。通过差分脉冲伏安法、循环伏安法、交流阻抗法和电流—时间曲线法优化自组装时间和信号探针的最佳响应浓度,研究目标基因的线性检测范围和再生性能。结果表明:修饰金电极最优自组装时间为15 h,信号探针的最佳响应浓度为0.51×10-6 mol/L,目标基因的线性检测范围为7.51×10-12-1.05×10-9 mol/L,检测下限为7.51×10-13 mol/L。该修饰电极具有良好的再生性能。 相似文献
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A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled
carbon nanotubes (MMCNT) which possess excellent capability of electron transfer were prepared by chemical coprecipitation
method. Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) were used to identify its surfacetopography
and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid
of chitosan/silica (CS) hybrid membrane. Using current-time detection method, the biosensor shows a linear response related
to the concentration of catechol in the range from 10−7 to 0.165×10−3 mol/L. The corresponding detection limit is 3.34×10−8 mol/L based on signal-to-noise ratios (S/N) ≥3 under the optimized conditions. In addition, its response current retains
90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop
other enzyme-based biosensors. 相似文献
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