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To effectively monitor the characteristic of Acidithiobacillus ferrooxidans ATCC 23270 at the whole-genomic level,a whole-genome 50-mer-based oligonucleotide microarray was developed based on the 3 217 ORFs of A.ferrooxidans ATCC 23270 genome.Based on artificial oligonucleotide probes,the results showed that the optimal hybridization temperature was 45℃. Specificity tests with the purified PCR amplifications of 5 genes(Sulfide-quinone reductase,Cytochrome C,Iron oxidase,Mercuric resistance protein,Nitrog... 相似文献
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