Development and evaluation of whole-genome oligonucleotide array for Acidithiobacillus ferrooxidans ATCC 23270

To effectively monitor the characteristic of Acidithiobacillus ferrooxidans ATCC 23270 at the whole-genomic level,a whole-genome 50-mer-based oligonucleotide microarray was developed based on the 3 217 ORFs of A.ferrooxidans ATCC 23270 genome.Based on artificial oligonucleotide probes,the results showed that the optimal hybridization temperature was 45℃. Specificity tests with the purified PCR amplifications of 5 genes(Sulfide-quinone reductase,Cytochrome C,Iron oxidase,Mercuric resistance protein,Nitrog...     

液体橡胶增韧改性中温固化环氧树脂研究及其碳纤维复合材料制备

通过测试端羟基丁腈橡胶-环氧树脂预混物在体系中的不同比例，发现预混物加入42.9%时，其增韧效果最佳，树脂拉伸强度、弯曲强度和断裂伸长率分别提升148.8%、159.4%和264.8%,树脂及其复合材料的玻璃化转变温度分别为132.8℃和138.9℃,并制备得到工艺性和力学性能较好的复合材料。通过动态DSC法，研究了增韧环氧树脂的固化反应动力学，并建立了该树脂的固化度-温度-时间三者关系的理论模型。采用拉丝法，拟合得到凝胶时间和温度之间的关系曲线，为环氧树脂及其预浸料的成型工艺提供理论指导。