Fatty acid desaturase systems of hen liver and their inhibition by cyclopropene fatty acids

Hen liver preparations which desaturate stearic acid at the 9,10 position to form oleic acid have been found to desaturate other saturated fatty acids of carbon chain length from 12 to 20 and 22. The 9,10-monoenoic fatty acid of the same chain length as the substrate fatty acid is the major product formed. Minor amounts of the 10,11- and 11, 12-monoenoic acids are also formed. Maximum desaturation occurred with the C₁₄ fatty acid substrate and with the fatty acids C₁₇ and C₁₈, suggesting the presence of at least two desaturating systems. The cyclopropene fatty acids, sterculic and malvalic acids, inhibited the desaturation of all thefatty acids at the 9,10 position but desaturation at the 10,11 and 11, 12 positions was affected only slightly. The effect is not due to inhibition of the primary activating enzyme, the long chain acyl CoA synthetase. Sterculic acid is a more effective inhibitor than either malvalic acid or sterculyl alcohol, probably because these cyclopropene compounds do not block the desaturating site of the enzyme as completely as sterculic acid.     

General Liability Insurance Coverage for Mold Claims

Although humans and mold have coexisted for centuries, the effects of mold on health and property have recently received considerable attention. Members of the construction industry and commercial property owners are, with increasing frequency, finding themselves defending claims of property damage or personal injury due to indoor mold growth. These designers, builders, and property owners frequently turn to their general liability insurance carriers for defense and indemnification without having a full understanding of the protections afforded by their commercial general liability policies. This article provides an introduction to the typical coverages and exclusions applicable to mold related claims submitted for general liability coverage. This article also discusses maximizing the potential for coverage by notifying and cooperating with one’s insurer and provides guidance on the avoidance of indoor mold growth and claims. Questions concerning the meaning and effect of particular insurance policy provisions and the availability of additional or alternative coverages should be directed to one’s insurance professional or coverage attorney.     

Survey of 1012 moldy dwellings by culture fungal analysis: Threshold proposal for asthmatic patient management

Different countries have tried to define guidelines to quantify what levels of fungi are considered as inappropriate for housing. This retrospective study analyzes indoor fungi by cultures of airborne samples from 1012 dwellings. Altogether, 908 patients suffering from rhinitis, conjunctivitis, and asthma were compared to 104 controls free of allergies. Portuguese decree law no 118/2013 (PDL118), ANSES (a French environmental and health agency) recommendations, and health regulations of Besançon University Hospital were applied to determine the rates of non‐conforming dwellings, which were respectively 55.2%, 5.2%, and 19%. Environmental microbiological results and medical data were compared. The whole number of colonies per cubic meter of air was correlated with asthma (P < 0.001) and rhinitis (P = 0.002). Sixty‐seven genera and species were detected in bedrooms. Asthma was correlated to Aspergillus versicolor (P = 0.004) and Cladosporium spp. (P = 0.02). Thresholds of 300 cfu/m³ for A. versicolor or 495 cfu/m³ for Cladosporium spp. are able to discriminate 90% of the asthmatic dwellings. We propose a new protocol to obtain an optimal cost for indoor fungi surveys, excluding surface analyses, and a new guideline to interpret the results based on >1000 cfu/m³ of whole colonies and/or above threshold levels for A. versicolor or Cladosporium spp.     

Analysis of indoor particles and gases and their evolution with natural ventilation

The air composition and reactivity from outdoor and indoor mixing field campaign was conducted to investigate the impacts of natural ventilation (ie, window opening and closing) on indoor air quality. In this study, a thermal desorption aerosol gas chromatograph (TAG) obtained measurements of indoor particle‐ and gas‐phase semi‐ and intermediately volatile organic compounds both inside and outside a single‐family test home. Together with measurements from a suite of instruments, we use TAG data to evaluate changes in indoor particles and gases at three natural ventilation periods. Positive matrix factorization was performed on TAG and adsorbent tube data to explore five distinct chemical and physical processes occurring in the indoor environment. Outdoor‐to‐indoor transport is observed for sulfate, isoprene epoxydiols, polycyclic aromatic hydrocarbons, and heavy alkanes. Dilution of indoor species is observed for volatile, non‐reactive species including methylcyclohexane and decamethylcyclopentasiloxane. Window opening drives enhanced emissions of semi‐ and intermediately volatile species including TXIB, DEET, diethyl phthalate, and carvone from indoor surfaces. Formation via enhanced oxidation was observed for nonanal and 2‐decanone when outdoor oxidants entered the home. Finally, oxidative depletion of gas‐phase terpenes (eg, limonene and α‐pinene) was anticipated but not observed due to limited measurement resolution and dynamically changing conditions.     

Review of Bioretention System Research and Design: Past, Present, and Future

This paper reviews the evolution of bioretention systems, a promising at-source storm-water best management practice. The introduction of bioretention systems in the 1990s by Prince George’s County, Md. is examined along with the motivations behind the development of the systems. A summary of the research findings on the performance of bioretention systems is provided including proposed design modifications to improve the field performance. Also included is an overview of past and current bioretention design guidelines in North America, as well as a discussion of issues surrounding the public adoption and implementation of bioretention systems. Potential alternative uses for the systems are highlighted and a review of bioretention modeling work is provided. Finally, the paper outlines research needs and anticipated future work necessary to bring about the widespread use of bioretention systems.