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1.
Dr. Qian Wang Xinli Fan Nannan Jing Han Zhao Dr. Lijia Yu Prof. Xinjing Tang 《Chembiochem : a European journal of chemical biology》2021,22(11):1901-1907
Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes. 相似文献
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针对目前运动想象脑电(EEG)信号识别率较低的问题,考虑到脑电信号蕴含着丰富的时频信息,提出一种基于时频域的卷积神经网络(CNN)运动想象脑电信号识别方法。首先,利用短时傅里叶变换(STFT)对脑电信号的相关频带进行预处理,并将多个电极的时频图组合构造出一种二维时频图;然后,针对二维时频图的时频特性,通过一维卷积的方法设计了一种新颖的CNN结构;最后,通过支持向量机(SVM)对CNN提取的特征进行分类。基于BCI数据集的实验结果表明,所提方法的平均识别率为86.5%,优于其他传统运动想象脑电信号识别方法;同时将该方法应用在智能轮椅上,验证了其有效性。 相似文献
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Human tracking has been a challenging task for robot in the past decades. In this paper, to realize the human following in a cluttered environment, a human tracking system based on adaptive multi-feature mean-shift (AMF-MS) under the double-layer locating mechanism (DLLM) is proposed to solve the problem of distinguishing target, occlusion, and quick turning. The DLLM, considering the course location processing and fine location processing, is designed to estimate the person’s position using the fusion of heterogeneous data. As an ID tag attached on target can be detected by RF antennas, the course locating method can track the target easily and quickly. The Bayes rule is introduced to calculate the probability where the tag exists due to the instability of RF signals. In the fine locating step, the AMF-MS is proposed because it can reduce computational load and represent target by multi-feature histogram function. Meanwhile, we combine extended Kalman filter and AMF-MS to overcome MS’s inability of occlusion. To control the robot following the target person precisely, an intelligent gear shift strategy based on fuzzy control is implemented by analyzing the robot structure. Experiments demonstrate that the proposed approach is robust to handle complex tracking conditions, and show the system has an optimum performance. 相似文献
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The effects of the molecular weights (molecular weight of polystyrene, Mw,PS, varying from 2.9 to 129 k) on the surface morphologies of spin-coated and annealed polystyrene/poly (methyl methacrylate) (PS/PMMA=50/50, w/w) blend films were investigated by atomic force microscopy and X-ray photoelectron spectroscopy. For the spin-coated films, when the Mw,PS varied from 2.9 to 129 k, three different kinds of surface morphologies (a nanophase-separated morphology, a PMMA cellular or network-like morphology whose meshes filled with PS, a sea-island like morphology) were observed and their formation mechanisms are discussed, respectively. Upon annealing, two different morphology-evolution processes were observed. It is found that a upper PS-rich phase layer is formed when Mw,PS<4 k, and this behavior is mainly attributed to the low interfacial tension between PS and PMMA component. When Mw,PS>4 k, the PS-rich phase forms droplets on top of the PMMA-rich phase layer which wets the SiOx substrate. These results indicate that the surface morphology of the polymer blend films can be controlled by the polymer molecular weight and annealing conditions. 相似文献
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The effects of the glass‐bead content and size on the nonisothermal crystallization behavior of polypropylene (PP)/glass‐bead blends were studied with differential scanning calorimetry. The degree of crystallinity decreased with the addition of glass bead, and the crystallization temperature of the blends was marginally higher than that of pure PP at various cooling rates. Furthermore, the half‐time for crystallization decreased with an increase in the glass‐bead content or particle size, implying the nucleating role of the glass beads. The nonisothermal crystallization data were analyzed with the methods of Avrami, Ozawa, and Mo. The validity of various kinetic models for the nonisothermal crystallization process of PP/glass‐bead blends was examined. The approach developed by Mo successfully described the nonisothermal crystallization behavior of PP and PP/glass‐bead blends. Finally, the activation energy for the nonisothermal crystallization of pure PP and PP/glass‐bead blends based on the Kissinger method was evaluated. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 2026–2033, 2006 相似文献
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为使图像加密系统具备优化功能,并解决当前遗传算法无法实现全局最优、收敛速率慢等问题,提出奇偶树型交互学习机耦合全局离散遗传算法的密文优化系统。定义权值更新机制,耦合混沌映射,构造奇偶树型交互学习机及其互扰模型。将切断型轮盘赌择取机制引入均匀交叉算子中,以图像分块的相邻像素相关系数和密文信息熵为目标,根据权重理论设计加权适应度函数,提出一种全局离散遗传算法,最终形成"初始加密-密文优化"的加密结构。实验结果表明,与超混沌算法、离散遗传算法、元胞自动机相比,该系统的加密质量较好,并且具备全局优化功能,可优化所有迭代结果,使最终输出密文的信息熵最大,相关系数最小。 相似文献
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HA/TCP and HA rods (?5 mm×10 mm) were made for implantation in New Zealand white rabbit with different condition. Sixty three rabbit were divided into three groups: group 1 (n=18), group 2 (n=27) and group 3 (n=18). In group 1, 10 mm radius was defected, and one HA/TCP rod was implanted in the muscle a distant away from the bone defect area. In group 2, also, 10 mm radius was defected, one HA rod was implanted in the muscle a distant away from the bone defect area. In group 3, two HA/TCP rods were implanted in the dorsal muscle of the rabbit with bone intact. Histological observation showed that in group 1, some new bone was found only two months after implantation (n=2), and obvious immature woven bone could be observed in these bioceramics from the 3rd month on. However, in group 3, bone began to be found 6 months after implantation (n=2). In group 2, we could not find any bone tissue up to 9 month’s observation. These results suggest that, first, the bone defect model could significantly accelerate bone formation at non-osseous sites in rabbits; second,. HA/TCP bioceramics were confirmed with osteoinductive property while HA bioceramics without osteoinductive property nearly. Thus, bone defect might be a good animal model for further researches for osteoinductive bioceramics. 相似文献