High performance liquid chromatographic separation of stereoisomeric bile acids as their UV-sensitive esters

High performance liquid chromatographic separation of a series of mono-, di- and trihydroxylated 5β-cholanic acids which differ only in position and configuration of hydroxyl groups at positions C-3, C-7 and/or C-12, is reported. The C-24 free acids were derivatized to four different classes of UV-sensitive esters, i.e.,p-bromophenacyl (BP),m-methoxyphenacyl (MP), 4-nitrophthalimidemethyl (NPM) and 9-anthrylmethyl (AM) esters, and chromatographed on two, variants of C₁₈ reversed-phase columns (Nova-Pak C₁₈ and Zorbax ODS) with methanol-water systems as mobile phase. Separation efficiency and elution order of some isomeric pairs were influenced by both the structure of the C-24 ester groups and the nature of the columns used. Excellent chromatographic properties were found for those derivatives, particularly for the NPM esters.     

Multiple uses of Essential Oil and By-Products from Various Parts of the Yakushima Native Cedar (Cryptomeria Japonica)

This article presents multiple potential uses of the Yakushima native cedar (Cryptomeria japonica), known in Japan as the Yaku-sugi tree. The Yaku-sugi was divided into four parts: leaves, branches with leaves, branches, and stems. We obtained the essential oil, hydrosol, distillation residue, and distillation wastewater from the trees. Essential oil and hydrosol were determined to be volatile organic compounds. All samples were screened for the following bioactivities: antioxidative, antibacterial, and anti-melanogenesis activities. Freeze-dried distillation residue was tested to assess whether it had a deodorizing effect. The main component of the leaf essential oil was found to be monoterpenes. In contrast, the stem essential oil mainly contained sesquiterpenes. In terms of bioactivities, the leaf essential oil showed antibacterial activity and the stem essential oil showed anti-melanogenesis activity. Distillation residue and wastewater showed many activities, including antioxidant, antibacterial, and anti-melanogenesis activities. Moreover, the residue had a deodorizing effect against ammonia.     

A single oral administration of conjugated linoleic acid enhanced energy metabolism in mice

We investigated the effect of a single oral administration of conjugated linoleic acid (CLA) on energy metabolism in mice. Male Std ddY mice were orally administered CLA (5 mL/kg weight) or linoleic acid (5 mL/kg weight) (both solutions at concentrations of 73.5%) as a control. Oxygen consumption was significantly greater in the CLA-administered mice than in the control mice. Respiratory quotient was slightly lower in the CLA-administered mice than in the control mice. We calculated fat and carbohydrate oxidation from oxygen consumption and respiratory quotient. Fat oxidation in the CLA-administered mice was significantly higher than in the control mice, and there was no difference in carbohydrate oxidation. Serum concentrations of noradrenalin and adrenalin in the CLA administered mice were significantly higher than in the control mice. These results suggested that CLA enhanced sympathetic nervous activity and energy metabolism.     

Transmission electron microscopic observations of the decomposition process of lithium alanate

The thermal decomposition process of lithium alanate (LiAlH₄) was investigated by TEM, TG-DTA and XRD. It was shown that LiAlH₄ decomposes through a two-step reaction: a liquid-to-solid phase transition in the first step of the decomposition and a solid-to-solid reaction in the second step of the decomposition, both steps accompanied by hydrogen release. The particle size of the aluminum (Al), which formed in the first decomposition step, was much larger than that in the second decomposition step. In addition, Al particles formed in the liquid phase of LiAlH₄ in the first decomposition step, while, they form in solid phase of Li₃AlH₆ in the second decomposition step, resulting in the kinetics of the first decomposition step being faster than that of the second decomposition step. The investigation clearly demonstrated the reaction model of the decomposition of LiAlH₄ in the nano-scale, showing different diffusion processes of Al in each of the decomposition steps.     

Variable temperature neutron diffraction studies of single crystals of LiND2

We have synthesized a single crystal of lithium amide (LiNH₂, LiND₂) by melting method, and performed neutron diffraction of the single crystal at variable temperature. LiND₂ is tetragonal structure and I-4 space group. Lattice parameters and unit cell volume of LiND₂ at room temperature, 50 °C, 100 °C, 150 °C and 200 °C were determined. Both of the lattice parameters and the unit cell volume increase with increase of temperature. From these results, we have estimated coefficient of volumetric thermal expansion α_V of LiND₂ to be 222 × 10⁻⁶/K. With increase of temperature, all thermal ellipsoids gradually expand because of thermal vibration.     

Thermal Feasibility Analyses for the 1356MWe High Conversion-Type Innovative Water Reactor for Flexible Fuel Cycle

A new reactor concept of innovative water reactor for flexible fuel cycle (FLWR) is under development at Japan Atomic Energy Agency in cooperation with Japanese reactor suppliers. A design of 1,356 MWe high conversion boiling water reactor-type FLWR core, which has an instantaneous conversion ratio of 1.04, negative void coefficient, high burnup of 65 GWd/t, and 15-month operational cycle length, has been constructed. So far, studies on thermal-hydraulic characteristics have been performed for tight lattice core. Evaluation methods for the critical power and the pressure drop under both the steady and the transient states have been established, and a modified TRAC-BF1 code has been developed for the thermal-hydraulic design of the FLWR. In this paper, the thermal feasibility of the designed 1356MWe FLWR core is analyzed by using the modified TRAC-BF1 code. The analysis is first carried out for the current core design. It is confirmed that no boiling transition (BT) occurs under the steady state. However, the minimum critical power ratio (MCPR) is only about 1.08, and the BT is confirmed occurring under the postulated abnormal transient processes. Therefore, concretizations of the conditions that ensure the thermal feasibility of a natural circulation-type FLWR and a forced circulation-type FLWR are performed. As for the results, for a forced circulation-type FLWR, the operation-limited MCPR (OLMCPR) is 1.32, and the necessary minimum core coolant flow rate is 640 kg/(m²s). For a natural circulation-type FLWR, the OLMCPR is 1.19, and the necessary minimum core coolant flow rate is 560 kg/(m²s).     

MRI findings of VIIth cranial nerve involvement in sarcoidosis

Right facial nerve palsy in a 58-year-old woman was due to sarcoidosis demonstrated by Gd-DTPA enhanced MRI. Abnormal enhancement of the right VIIth cranial nerve in the distal internal acoustic canal was seen on MRI. The enhancing lesion was smaller after 1 month of prednisolone 50 mg day-1. This is the first report on facial nerve involvement in neurosarcoidosis examined by Gd-DTPA enhanced MRI. The use of Gd-DTPA enhanced MRI with thin slicing, e.g. 3 mm slice thickness and 1 mm interslice gap, is effective in detecting small extramedullary lesions.     

Numerical accuracy of multipole expansion for 2D MLFMA

A numerical study of the multipole expansion for the multilevel fast multipole algorithm (MLFMA) is presented. In the numerical implementation of MLFMA, the error comes from three sources: the truncation of the addition theorem; the approximation of the integration; the aggregation and disaggregation process. These errors are due to the factorization of the Green's function which is the mathematical core of the algorithm. Among the three error sources, we focus on the truncation error and a new approach of selecting truncation numbers for the addition theorem is proposed. Using this approach, the error prediction and control can be improved for the small buffer sizes and high accuracy requirements.