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1.
Tadashi Hano Michiaki Matsumoto Takahisa Kawazu Takaaki Ohtake 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1995,62(1):60-63
The extraction equilibria of various di- and tripeptides with di-2-ethylhexylphosphoric acid (D2EHPA) were studied at low pH values. The complex extracted to organic phase consisted of one molecule of peptide and two molecules of D2EHPA dimer. The extraction constants of the peptides correlated well with the distribution coefficients of peptides between 1-octanol and water, which is a measure of hydrophobicity. The permeation rates of peptides through an emulsion liquid membrane were examined by using D2EHPA as a carrier, Span 80 as an emulsifier and kerosene as a diluent. The rates varied considerably with peptide type, depending upon the hydrophobicity. 相似文献
2.
Of the four genes (nrtABCD) required for active transport of nitrate in the cyanobacterium Synechococcus sp. strain PCC 7942, nrtBCD encode membrane components of an ATP-binding cassette transporter involved in the transport of nitrite as well as of nitrate, whereas nrtA encodes a 45-kDa cytoplasmic membrane protein, the biochemical function of which remains unclear. Characterization of the nrtA deletional mutants showed that the 45-kDa protein is essential for the functioning of the nitrate/nitrite transporter. A truncated NrtA protein lacking the N-terminal 81 amino acids, expressed in Escherichia coli cells as a histidine-tagged soluble protein, was shown to bind nitrate and nitrite with high affinity (Kd = 0.3 microM). Immunoblotting analysis using the antibody against the 45-kDa protein revealed a 48-kDa precursor of the protein, which accumulated in the cyanobacterial cells treated with globomycin, an antibiotic that specifically inhibits cleavage of the signal peptide of lipoprotein precursors. These findings indicated that the nrtA gene product is a nitrate- and nitrite-binding lipoprotein. The N-terminal sequences of putative cyanobacterial substrate-binding proteins suggested that lipoprotein modification of substrate-binding proteins of ATP-binding cassette transporters is common in cyanobacteria. 相似文献
3.
Mixed oxide catalyst prepared by co-precipitating magnesium oxide and calcium oxide showed an excellent activity for the oxidative coupling of methane. The high performances were presumed to arise from the high basicity of the mixed oxide. 相似文献
4.
N,N'- Unsymmetrical dialkyl-3,4:9,10-perylenebis(dicarboximide)s (in which alkyl = methyl, ethyl, propyl, butyl, isobutyl, pentyl, hexyl and octyl) were prepared by the condensation of N-alkyl-3,4:9,10-perylene-tetracarboxylic monoanhydride monoimide with the appropriate alkyl-amines. The properties of these derivatives as pigments were tested and their thermal stability measured. 相似文献
5.
N′-Alkyl-N′-aryl-3,4:9,10-perylenebis(dicarboximide) (alkyl = isobutyl, pentyl, hexyl, octyl, etc.; aryl = phenyl, p-tolyl, p-methoxyphenyl, etc.) were prepared by the condensation of N-alkyl-3,4:9,10-perylene-tetracarboxylic monoanhydride monoimide with arylamines (aniline, p-toluidine, p-anisidine, o-phenylenediamine, etc.). The properties of these derivatives as pigments were tested, and also the thermal stability of 3,4:9,10-perylenebis(dicarboximide) derivatives was measured. 相似文献
6.
An ozone generator using a rotating electrode to improve ozone generation efficiency is proposed. The ozone generator electrode unit consists of a rotating electrode and fixed electrode. The rotating electrode has the grounded 36 pieces of tungsten wires fixed in parallel to the rotation axis on the rotating cylinder surface. A dielectric electrode is used as a fixed electrode located on the inside of the tube of the electrode unit. The width of the apparent discharge gap is 1mm. Alternating current with a frequency of 50 Hz is applied to the electrode unit. The rotation speed can be adjusted from 0 rpm to 1200 rpm by a variable speed motor. Oxygen gas is used as the material gas. Higher ozone concentration and higher ozone generation efficiency are obtained compared with that when the rotation speed is 0 rpm. The gas temperature is measured at the inlet and outlet of the ozone generator, and the rotation speed for the cooling effect is most effective at about 500 rpm. The maximum generation efficiency is estimated to be 61 g/kWh at 800 rpm, and this value is twice as large as in the case of 0 rpm. 相似文献
7.
S Kawai F Imazeki O Yokosuka M Ohto S Shiina N Kato M Omata 《Canadian Metallurgical Quarterly》1995,22(1):112-117
Analysis of X-chromosome inactivation patterns in women has been used to assess the clonality of various tumors. In this report, we analyzed 27 liver tumors in women, including 18 samples obtained by the performance of ultrasonically guided thin-needle biopsies. By analysis of the heterogeneity of phosphoglycerate kinase gene, 11 of 27 (41%) cases were found to be heterozygous at the gene. Of these informative 11 cases with liver tumors, 7 cases were "large" tumors (> 25 mm in diameter) and 4 cases were "small" tumors (< 25 mm in diameter). All 7 large tumors showed monoclonal patterns by the phosphoglycerate kinase gene analysis. Of the 4 small tumors, 2 showed monoclonal, and 2 showed polyclonal patterns. The 2 with monoclonal patterns were pathologically diagnosed as hepatocellular carcinoma despite their small sizes (20 mm and 23 mm). Of the two with polyclonal patterns, the smallest one (15 mm) was diagnosed as benign adenomatous hyperplasia, and the other as hepatocellular carcinoma heavily infiltrated by lymphocytes. These data suggest that analysis of the methylation pattern of the phosphoglycerate kinase gene may be helpful on rare occasions in elucidating the nature of liver tumors but must in fact be used in conjunction with histological appearances to avoid errors secondary to inflammatory infiltrates. 相似文献
8.
Naoki Motomura Yuto Yamazaki Daiki Koga Shogo Harashima Xin Gao Yuta Tezuka Kei Omata Yoshikiyo Ono Ryo Morimoto Fumitoshi Satoh Yasuhiro Nakamura Go Eun Kwon Man Ho Choi Akihiro Ito Hironobu Sasano 《International journal of molecular sciences》2022,23(4)
Cortisol-producing adenoma (CPA) is composed of clear and compact cells. Clear cells are lipid abundant, and compact ones lipid poor but associated with higher production of steroid hormones. PRKACA mutation (PRKACA mt) in CPA patients was reported to be associated with more pronounced clinical manifestation of Cushing’s syndrome. In this study, we examined the association of histological features and genotypes with cholesterol uptake receptors and synthetic enzymes in 40 CPA cases, and with the quantitative results obtained by gas chromatography-mass spectrometry (GC-MS) analysis in 33 cases to explore their biological and clinical significance. Both cholesterol uptake receptors and synthetic enzymes were more abundant in compact cells. GC-MS analysis demonstrated that the percentage of compact cells was inversely correlated with the concentrations of cholesterol and cholesterol esters, and positively with the activity of cholesterol biosynthesis from cholesterol esters. In addition, hormone-sensitive lipase (HSL), which catalyzes cholesterol biosynthesis from cholesterol esters, tended to be more abundant in compact cells of PRKACA mt CPAs. These results demonstrated that both cholesterol uptake and biosynthesis were more pronounced in compact cells in CPA. In addition, more pronounced HSL expression in compact cells of PRKACA mt CPA could contribute to their more pronounced clinical manifestation. 相似文献
9.
Lianqi Zhang Hideyuki Noguchi Decheng Li Takahisa Muta Xiaoqing Wang Masaki Yoshio Izumi Taniguchi 《Journal of power sources》2008
On the basis of extreme similarity between the triangle phase diagrams of LiNiO2–LiTiO2–Li[Li1/3Ti2/3]O2 and LiNiO2–LiMnO2–Li[Li1/3Mn2/3]O2, new Li–Ni–Ti–O series with a nominal composition of Li1+z/3Ni1/2−z/2Ti1/2+z/6O2 (0 ≤ z ≤ 0.5) was designed and attempted to prepare via a spray-drying method. XRD identified that new Li–Ni–Ti–O compounds had cubic rocksalt structure, in which Li, Ni and Ti were evenly distributed on the octahedral sites in cubic closely packed lattice of oxygen ions. They can be considered as the solid solution between cubic LiNi1/2Ti1/2O2 and Li[Li1/3Ti2/3]O2 (high temperature form). Charge–discharge tests showed that Li–Ni–Ti–O compounds with appropriate compositions could display a considerable capacity (more than 80 mAh g−1 for 0.2 ≤ z ≤ 0.27) at room temperature in the voltage range of 4.5–2.5 V and good electrochemical properties within respect to capacity (more than 150 mAh g−1 for 0 ≤ z ≤ 0.27), cycleability and rate capability at an elevated temperature of 50 °C. These suggest that the disordered cubic structure in some cases may function as a good host structure for intercalation/deintercalation of Li+. A preliminary electrochemical comparison between Li1+z/3Ni1/2−z/2Ti1/2+z/6O2 (0 ≤ z ≤ 0.5) and Li6/5Ni2/5Ti2/5O2 indicated that charge–discharge mechanism based on Ni redox at the voltage of >3.0 V behaved somewhat differently, that is, Ni could be reduced to +2 in Li1+z/3Ni1/2−z/2Ti1/2+z/6O2 while +3 in Li6/5Ni2/5Ti2/5O2. Reduction of Ti4+ at a plateau of around 2.3 V could be clearly detected in Li1+z/3Ni1/2−z/2Ti1/2+z/6O2 with 0.27 ≤ z ≤ 0.5 at 50 °C after a deep charge associated with charge compensation from oxygen ion during initial cycle. 相似文献
10.
Tajima T Takiguchi N Kato J Ikeda T Kuroda A Ohtake H 《Journal of Bioscience and Bioengineering》2003,96(2):149-153
The nematode Caenorhabditis elegans exhibits chemotaxis toward a wide variety of chemicals including water-soluble molecules and volatile organic compounds. We have previously discovered that C. elegans wild-type strain N2 is strongly attracted by cycloheximide which has long been known as a bitter tastant for humans and other mammals. We describe here the isolation and initial characterization of the first mutants which were defective specifically in their attraction to cycloheximide. In our screenings, we selected two mutants that were defective in chemotaxis to cycloheximide but normal in their attraction to NH4Cl and histidine. These mutants also avoided quinine hydrochloride, CuSO4 and high concentrations of NaCl similar to the wild-type strain N2. Furthermore, no observable defect was detected in their attraction to volatile odorants such as isoamyl alcohol and diacetyl. Dye-filling experiments suggested that they have no morphological defect in the sensory endings of the amphid neurons. 相似文献