液相色谱-串联质谱法同时测定血液中的18-甲基炔诺酮和孕酮含量

A selective, reliable and rapid method for the simultaneous determination of progesterone and norgestrel concentration in human serum after taking oral contraceptive tablet has been developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with atmospheric pressure chemical ionization (APCI) interface. The norethisterone was used as the internal standard. Selected transitions of m/z 313/245 for norgestrel, m/z 315/97 for progesterone and m/z 299/109 for norgestrel were monitored using multiple reaction monitoring (MRM) mode for quantitation. The assay was linear over the concentration range of 0.2-50ng/mL for norgestrel and progesterone. The lower level of quantitation in human serum was obtained at 0.2ng/mL for both norgestrel and progesterone using optimum tuning parameters. Serum concentrations of norgestrel and progesterone were determined following oral administration of norgestrel to female volunteers.     

波相色谱-质谱联用技术

本文简要介绍了LC-MS及其接口的基本原理技术和发展,对影响LC-MS分析结果的诸多因素作了一一说明。并对常见的质谱技术和仪器进行了比较。     

LC/MS分析4,4′庚基联苯腈中杂质

利用带有喷雾-传送带接口的液相色谱-质谱联用仪分析了4,4′庚基联苯腈中的杂质。鉴定了其中的三种杂质,并对实验结果进行了讨论。     

中药复方中柴胡皂甙A和D的LC／MS／MS研究

To study Saikosaponins in a Chinese traditional compound medicine by LC/MS. Saikosaponin A and D, which were the bioactive components of Bupleurum falcatum L., were not found in the medicine. And three isomeric compounds of Saikosaponin A and D were detected instead. Saikosaponin A and D were found have turned into two other isomers after treated by acid water, but keep unchanged when treated by neutral water. Two of the isomeric compounds which found in the Compound medicine were determined as Saikosaponin B1 and B2. This work is helpful for quality control of the medicine and also is useful for supervising the procedures of manufacture.     

用色谱/质谱联用法分析研究CFCs及其替代物

本文介绍了利用色谱/质谱联用法研究电冰箱中制冷剂和发泡剂的CFCs及其替代物,建立了分析方法、并对十几种CFCs及其替代物的成分进行了分析。     

二次离子质谱法在有机物分析中的应用

二次离子质谱法(SIMS)是一种分析易热解和不挥发的有机化合物的新技术。本文介绍了SIMS的装置、样品制备、二次离子产生机理以及在肽类、氨基酸、维生素、药物等分析中的应用。