液相色谱-串联质谱法测定人血浆中克拉霉素的浓度

A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to determine clarithromycin in human plasma. The linearity was obtained in the range of 10~5 000 ng/mL in plasma and the limit of quantification was 10 ng/mL. The intra and inter-day precision (RSD) were less than 2.9% and 9.2%, respectively. Accuracy(relative error)was with in 3.2%. The method was proved to be convenient, rapid and accurate enough to be applied to the pharmacokinetic studies of clarithromycin in human plasma.     

液相色谱-串联质谱法测定玉米中伏马菌素B1

Fumonisin B1 in maize was determined by liquid chromatography/tandem mass spectrometry. The analysis was extracted from maize samples by acetonitrile-water-acetic acid 49:50:1(V/V/V). Raw extracts were evaporated to dryness and dissolved with mobile phase, chromatographyed on a C₁₈ column, and detection was performed on a triple quadrupole tandem mass spectrometry with electrospray ionization source. The method has a limit of quantification of 10 µg•kg^－1 in maize samples. The calibration curve is linear in the range of 1-500 µg•L^－1. The mean recovery ranges from 94 % to 104 %, and the relative standard derivations are lower than 9%. The method is sensitive and specific to be applied for determination of fumonisin B1 in maize samples.     

HPLC-MS/MS测定油炸及高温烘烤食品中的丙烯酰胺

为检测食品中的丙烯酰胺,建立了饼干、面包、油条、薯片、咖啡多种食品中丙烯酰胺的测定方法。试样经水溶液振荡提取,硅藻土柱净化,采用HPLCMSMS和同位素稀释定量技术,以选择反应监测(SRM)方式测定目标化合物。方法的检出限和定量限分别为4μgkg和12μgkg,线性范围10～3000μgL,不同食品基质的不同加标水平(20～2000μgkg)的回收率为90%～105%,RSD<10%。本方法定量准确、可靠,适用于食品中丙烯酰胺的测定。     

液液萃取-液相色谱-串联质谱法测定蜂蜜中氯霉素

目的建立液相色谱-串联质谱法检测蜂蜜中氯霉素残留量的方法。方法试样前处理采用液液萃取,以甲醇 水(71 29体积分数)为流动相,用Zorbax Eclipse XDB C18柱进行分离,以氘代氯霉素作为内标,采用负离子方式,液相色谱-电喷雾串联质谱法进行测定。结果平均回收率为93.0%-98.2%,相对标准偏差小于8%。采用空白蜂蜜基质制作校正曲线,蜂蜜中氯霉素检测的线性范围为0.01-1.00μg/kg,定量检测下限为0.01μg/kg。结论方法灵敏、准确、快速,能满足蜂蜜中氯霉素的监督检测。