气相色谱-燃烧-同位素比值质谱法测定鳀鱼肌肉组织中单体氨基酸的碳稳定同位素组成

The stable carbon isotope compositions of individual amino acids in muscle tissues of the anchovy, Engraulis japonicus, a key species of animal in the Yellow and the East China Sea ecosystem, were determined by the gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Eleven amino acid compounds were identified and their δ(13C) values were successfully measured. The new ability to measure stable carbon isotope compositions of individual amino acids offers a potential method for understanding digest, absorption and metabolism of anchovy, Engraulis japonicus, pathway of carbon transfer through food web and evaluating trophic quality of diet.     

天然存在的碳、氮稳定同位素在生态系统研究中的应用

简要介绍稳定碳、氮同位素在生态系统领域中关于系统的碳源、能量流动、营养结构、污染物的生物放大作用 ,对系统稳定性变化的应用研究作了较为系统的论述 ,并对稳定碳、氮同位素在赤潮研究、环境污染治理、生态动力学建模及有机分子化合物系列示踪技术等方面的应用提出展望。     

气相色谱-燃烧-同位素比值质谱法测定单体氨基酸的碳稳定同位素组成

活性生物或化石中单体氨基酸的稳定同位素组成分析在生物地球化学研究中具有重要意义.可应用于解释前寒武纪的生物演化[1];确定原始人类的古食性趋势[2];探索地质和生物体系中氨基酸的来源和成岩的历史变化情况[3,4];评价含碳陨石中有机物的来源[5,6];解释复杂生态系统中的微生物过程[7];了解氨基酸的代谢同化作用[8]等等.     

氨基酸在黄东海食物网中的传递

The transfer of amino acids in a key species “Chlorella sp.Calanus sinicus Engraulis japonicus” food chain was studied. It makes for understanding profoundly mechanism of substance and energy flow in marine food webs. Concentrations of amino acids in samples were analyzed by GC/MS after derivatization. Calanus sinicus plays a linchpin function in amino acid enrichment of this food chain and is suitable to growth of Engraulis japonicus rather than synthesized bait despite of the latter has higher amino acid concentrations.