中、日、欧的被试者对河川景观注视过程的比较研究

在景观认知·评价过程中,如果把眼球运动作为反映大脑所进行的视觉情报处理过程的外部参数,那么眼的运动方式就可能因评价者的生活经验、知识水平甚至价值观和思维方式的不同而产生差异。因此,可以设想被试者的社会文化背景的差异也有可能会从他们对景观的认知方式上得到体现。作为视觉认知中异域文化差异的比较研究之一,运用眼球运动跟踪仪对评价者的景观认知评价过程进行了跟踪测试,并试图通过对认知过程中的注视点分布、注视点数以及注视点的停留时间和注视内容的变化趋势这几个角度的分析比较来探讨社会文化背景的差异如何体现在人类视觉情报信息的收集整理的外部反应中,从而明确在地域特色景观的保留和营造上构成要素的布局方式的意义。     

Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

While fatty acids play essential roles in the physiology of the myocardium, conventional culture media contain little lipid. We previously revealed that rat neonatal myocardium mainly contains docosahexaenoic (DHA), linoleic (LA), and arachidonic (AA) acids as polyunsaturated fatty acids (PUFAs), and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal myocardium. In this study, we first assessed the effects of supplementation of DHA, LA, or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat cardiomyocytes. Based on this assessment, we then evaluated the effects of DHA or AA supplementation on mRNA expression and further directly measured the contractile force of cardiomyocytes with the supplementations. This study revealed that percentage change of contractile area was maximized under 20 μM DHA or 50 μM AA supplementation while LA supplementation did not affect this contraction index, and that a widespread upregulation tendency of the mRNA expression related to differentiation, maturity, fatty acid metabolism, and cell adhesion was seen in the cultured cardiomyocytes with supplementation of DHA or AA. In particular, upregulation of the gene expression of cellular adhesion molecules connexin43 and N-cadherin were remarkable, whereas the effects on differentiation and maturation were less pronounced. Correspondingly, the increase of the percentage change of the contractile area of cardiomyocyte clusters in culture dishes with the supplementations was significant, whereas the enhancement of the contractile force was modest. These results suggest that supplementation of DHA or AA to the fetal cardiomyocyte culture may play effective roles in preventing the de-differentiation of the cardiomyocytes in culture and that the enhancement of the contractile performance may be mainly attributed to the improvement of intercellular connection.