Determination of individual long-chain fatty acyl-CoA esters in heart and skeletal muscle

A method has been developed for determination of individual long-chain fatty acyl-CoA esters from heart and skeletal muscle using high performance liquid chromatography (HPLC). The esters were extracted from freezeclamped tissue of pig and rat hearts and rat skeletal muscle for analysis on a radially compressed C₁₈ 5μ reversephase column. Nine peaks in the extract with carbon chain lengths from C₁₂ to C₂₀ that subsequently disappeared on alkaline hydrolysis were identified. The major acyl-CoA peaks were 14∶1, 18∶2, 16∶0 and 18∶1 and additionally in rat heart 18∶0. Total long-chain acyl-CoA esters obtained by summation of the individual molecular species was 11.34±1.48 nmol/g wet wt. pig heart; 14.51±2.11 nmol/g wet wt. in rat heart, and 4.35±0.71 nmol/g wet wt. in rat skeletal muscle. These values were approximately 132% of those obtained using a separate procedure that measured total CoA by HPLC after alkaline hydrolysis of the esters. The described method demonstrates the quantitation of individual acyl-CoA species in muscle tissue. Therefore, it has a number of advantages in that it permits information to be obtained on the individual molecular species under various nutritional and metabolic conditions.     

The Genetic Heterogeneity among Different Mouse Strains Impacts the Lung Injury Potential of Multiwalled Carbon Nanotubes

Genetic variation constitutes an important variable impacting the susceptibility to inhalable toxic substances and air pollutants, as reflected by epidemiological studies in humans and differences among animal strains. While multiwalled carbon nanotubes (MWCNTs) are capable of causing lung fibrosis in rodents, it is unclear to what extent the genetic variation in different mouse strains influence the outcome. Four inbred mouse strains, including C57Bl/6, Balb/c, NOD/ShiLtJ, and A/J, to test the pro‐fibrogenic effects of a library of MWCNTs in vitro and in vivo are chosen. Ex vivo analysis of IL‐1β production in bone marrow‐derived macrophages (BMDMs) as molecular initiating event (MIE) is performed. The order of cytokine production (Balb/c > A/J > C57Bl/6 > NOD/ShiLtJ) in BMDMs is also duplicated during assessment of IL‐1β production in the bronchoalveolar lavage fluid of the same mouse strains 40 h after oropharyngeal instillation of a representative MWCNT. Animal test after 21 d also confirms a similar hierarchy in TGF‐β1 production and collagen deposition in the lung. Statistical analysis confirms a correlation between IL‐1β production in BMDM and the lung fibrosis. All considered, these data demonstrate that genetic background indeed plays a major role in determining the pro‐fibrogenic response to MWCNTs in the lung.     

Numerical Studies on the Seismic Retrofit of Bridges Using Shape Memory Alloys

Recent earthquakes have highlighted the seismic vulnerability of bridges due to excessive movements at the joints (in simply supported deck bridges) or concentration of seismic forces in a few piers (in multi-span continuous deck bridges). Steel-based seismic restrainers and viscous shock transmitters are used to limit the joint openings in simply supported deck bridges or to redistribute the seismic force among all piers in continuous deck bridges, respectively. Currently used devices, however, have some limitations such as large dimensions, no energy dissipation, possible large residual displacements, bad control of the force transmitted to the substructure (steel restrainers), large dimensions, difficulty of installation in existing structures, need of maintenance, and sensitivity to the earthquake characteristics (viscous shock transmitters). Shape memory alloys (SMAs) with superelastic behavior show the potential to overcome the limitations involved in the current technologies. In this article, a multi-performance seismic device based on superelastic SMA wires is proposed for the seismic retrofit of multi-span simply supported and continuous deck bridges. The effectiveness of the SMA devices is assessed through a number of nonlinear time-history analyses on two bridge structures representative of existing Italian highway bridges. Results are compared to the seismic response of the bridges in the as-built configuration.     

Machining feature-based similarity assessment algorithms for prismatic machined parts

This paper presents algorithms for identifying machined parts in a database that are similar to a given query part based on machining features. In this paper we only consider parts that are machined on 3-axis machining centers. We utilize reduced feature vectors consisting of machining feature access directions, feature types, feature volumes, feature dimensional tolerances and feature group cardinality as a basis for assessing shape similarity. We have defined a distance function between two sets of reduced feature vectors to assess the similarity between them from the machining effort point of view. To assess the similarity between the two parts, one set of reduced feature vectors is transformed in space using rigid body transformations with respect to the other set such that the distance between them is minimized. The distance between the two sets of aligned reduced feature vectors is used as a measure of similarity between the two parts. The existing machined parts are rank ordered based on the value of the distance with respect to the query part. The cost of previously machined parts that have a very small distance from the query part can be used as a basis for estimating the cost of machining the new part.     

Optimization of wide-band linear arrays

An optimization method is proposed for linear arrays to be used in ultrasound systems under wide-band operation. A fast algorithm, the threshold accepting, has been utilized to determine the element positions and weight coefficients of a linear array that generates a desired beam pattern. To reduce the computational burden in the optimization procedure, an efficient numerical routine for the beam pattern evaluation has been implemented. We address the optimization problem of both dense and sparse wideband arrays. In the first case, the goal is to minimize the side-lobe energy by varying the element weights; we compare the optimized beam pattern with that obtained with classical shading functions, showing that better results can be achieved with a wide-band optimization. We also consider the optimization of the layout (positions and weights) of a sparse linear array to achieve a desired beam pattern with a fixed or minimum number of array elements. The comparison of the proposed method with a narrow-band optimization algorithm is presented, showing that better performances (about -7 dB further reduction of the side-lobe level) can be achieved with a wide-band sparse array optimization. Further numerical simulations are given, showing that the proposed method yields better results than wideband sparse random arrays and periodic arrays with the same aperture width     

Comparison of test methods to assess pozzolanic activity

Assessment of the pozzolanic activity of cement replacement materials is increasingly important because of the need for more sustainable cementitious products. The pozzolanic activity of metakaolin, silica fume, coal fly ash, incinerated sewage sludge ash and sand have been compared using the Frattini test, the saturated lime test and the strength activity index test. There was significant correlation between the strength activity index test and the Frattini test results, but the results from these tests did not correlate with the saturated lime test results. The mass ratio of Ca(OH)₂ to test pozzolan is an important parameter. In the Frattini test and strength activity index test the ratio is approximately 1:1, whereas in the saturated lime test the ratio is 0.15:1. This explains why the saturated lime test shows higher removal of Ca(OH)₂ and why the results from this test do not correlate with the other test methods.     

Overlap-Based Cell Tracker

In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by the National Institute of Standards and Technology (NIST). This cell tracker has the ability to track cells across a set of time lapse images acquired at high rates based on the amount of overlap between cellular regions in consecutive frames. It is designed to be highly flexible, requires little user parameterization, and has a fast execution time.     

Laparoscopic hysterectomy in a case of male pseudohermaphroditism with persistent Müllerian duct derivatives

We describe laparoscopic diagnosis and treatment for a case of dysgenetic male pseudohermaphroditism with persistent Mullerian ducts. The patient, a 32 year old man, with a history of surgery for hypospadias and cryptorchidism during childhood, was referred because of anejaculation. He was of short stature, with male external genitalia composed of a small penis and hypoplastic testis (1 ml right, 6 ml left side). Plasma follicle stimulating hormone (FSH) was high (17 mUI/ml), testosterone low (1.9 ng/ml), and his karyotype was 46,XY. Pelvic ultrasound, nuclear magnetic resonance (NMR) and genitography disclosed a uterine-like structure with cavity communicating with the urethra. Laparoscopy and urethrocystoscopy confirmed the presence of a 4 cm uterus, which was removed endoscopically at the same time. A biopsy of the left gonad was also performed. The uterus contained endometrial tissue and was fibrotic. Histology of the left gonad showed spermatocytic arrest. We diagnosed dysgenetic male pseudohermaphroditism. Laparoscopy, in our opinion, is an optimal tool to diagnose and treat abnormal sexual conditions.     

Quantitative oxygen measurements in OMVPE Al x Ga1−x As grown by methyl precursors

Oxygen has always been considered to be a major contaminant in the organo-metallic vapor phase epitaxy (OMVPE) of Al _x Ga_1−x As. Oxygen incorporation has been invoked as a contributor to low luminescence efficiency, dopant compensation and degradation of surface morphology among other deleterious effects. This study presents quantitative measurements of oxygen concentration in nominally high purity Al _x Ga_1−x As. The oxygen concentration was measured as a function of alloy composition, growth temperature, andV/III ratio. Quantitative secondary ion mass spectroscopy (SIMS) measurements were used to determine the oxygen content as well as the carbon concentration in the film. The oxygen concentration increases with decreased growth temperature and V/III ratio while increasing superlinearly with Al content in the epitaxial layer.