Polymer nanochannels fabricated by thermomechanical deformation for single-molecule analysis

A simple method for fabricating nanoscale channels based on thermomechanical deformation of rigid polymer substrates is demonstrated. Polycarbonate preforms containing microchannels with cross-sectional dimensions on the order of tens of micrometers are controllably deformed to produce submicrometer dimensions. The reduced channel dimensions are achieved by heating the preform while applying a uniaxial tensile force to reduce channel cross sections through the Poisson effect. Nanochannels with circular or elliptical cross sections are defined by varying the channel position and preform geometry prior to deformation. Arrays of parallel nanochannels with critical dimensions down to 400 nm are described. Using the fabrication method, a nanochannel network is fabricated for the detection of single protein molecules via confocal fluorescence microscopy. The chip includes a detection channel with cross-sectional dimensions approaching the confocal volume dimensions of the detection optics and a larger adjacent reference channel used to optimize focusing. Detection of fluorescently labeled bovine serum albumin at 15 and 150 nM concentrations is presented, demonstrating the ability to perform single-molecule fluorescence measurements in polycarbonate chips using visible wavelengths for excitation and detection.     

3,5‐Bis(benzylidene)‐4‐oxo‐1‐phosphonopiperidines and Related Diethyl Esters: Potent Cytotoxins with Multi‐Drug‐Resistance Reverting Properties

A series of 3,5‐bis(benzylidene)‐4‐piperidones 3 were converted into the corresponding 3,5‐bis(benzylidene)‐1‐phosphono‐4‐piperidones 5 via diethyl esters 4 . The analogues in series 4 and 5 displayed marked growth inhibitory properties toward human Molt 4/C8 and CEM T‐lymphocytes as well as murine leukemia L1210 cells. In general, the N‐phosphono compounds 5 , which are more hydrophilic than the analogues in series 3 and 4 , were the most potent cluster of cytotoxins, and, in particular, 3,5‐bis‐(2‐nitrobenzylidene)‐1‐phosphono‐4‐piperidone 5 g had an average IC₅₀ value of 34 nM toward the two T‐lymphocyte cell lines. Four of the compounds displayed potent cytotoxicity toward a panel of nearly 60 human tumor cell lines, and nanomolar IC₅₀ values were observed in a number of cases. The mode of action of 5 g includes the induction of apoptosis and inhibition of cellular respiration. Most of the members of series 4 as well as several analogues in series 5 are potent multi‐drug resistance (MDR) reverting compounds. Various correlations were noted between certain molecular features of series 4 and 5 and cytotoxic properties, affording some guidelines in expanding this study.     

Cryosurgical ablation of the normal ventral prostate plus adjuvant does not protect Copenhagen rats from Dunning prostatic adenocarcinoma challenge

PURPOSE: We wished to determine if cryosurgical ablation of the normal ventral prostate of Copenhagen rats confers protective immunity against a subsequent challenge with Dunning R3327 MatLyLu prostatic adenocarcinoma. In human melanoma, tumor antigens have been characterized as normal cellular proteins. We reasoned that cryosurgical ablation of the normal prostate along with immunostimulatory adjuvants might release prostatic antigens to the immune system engendering an immune response and rendering rats immune to prostatic cancer cells. MATERIALS AND METHODS: On day 0, Copenhagen rats underwent cryosurgical ablation of the normal ventral prostate, cryosurgery and intraprostatic injection of Complete Freund's Adjuvant (CFA), CFA injection alone, or laparotomy alone. On day 21, animals received a subcutaneous challenge of MatLyLu tumor cells. Tumor dimensions were recorded at regular intervals by a single blinded investigator. RESULTS: Animals receiving cryosurgical ablation of the normal ventral prostate or intraprostatic CFA developed tumors more frequently than animals receiving laparotomy alone and the effect was statistically significant if animals received both cryosurgical ablation of the prostate and intraprostatic CFA (3 experiments, 1 x 10(4) MatLyLu cells), total number with tumors/total number challenged: laparotomy alone 3/17, cryosurgical ablation 7/17, cryosurgery plus CFA 10/16 (p = 0.013 versus laparotomy, Fisher's exact test), CFA alone 9/17. CONCLUSIONS: Cryosurgical ablation of the normal rat ventral prostate and intraprostatic CFA does not protect against and can enhance the tumorigenicity of MatLyLu prostatic cancer cells at distant sites. This could be occurring through specific immunologic effects or non-specific mechanisms induced by cryosurgery and CFA.     

Fuzzy decoupling to reduce propagation of tension disturbances in roll-to-roll system

Control of web tension is crucial for maintaining quality of products processed on roll-to-roll (R2R) system. An R2R system can be divided into different tension spans which interact with each other. But converting industries tend to neglect these interactions and use decentralized single-input–single-output (SISO) control approaches to deal with tension control. Multi-input–multi-output approaches have been reported in literatures but are practically not in use. Interaction between the various tension spans is unavoidable as they are all connected by a single web. Disturbances produced in a span tend to travel further downstream along the direction of web travel. When the number of spans is less or the disturbance amplitudes are small, this does not present a big challenge and simple SISO control is sufficient. But when the amplitudes of disturbances produced in processing is large—as is the case with printed electronics—or the number of spans is large or both, then the interactions cannot be neglected. R2R-based offset printers have the potential for mass production of precision-printed electronics. In this paper, a fuzzy logic-based hybrid approach has been followed that specifically targets the printed electronics industry and this method considerably reduces the interactions. The algorithm has been designed such that it takes information from previous span to reduce the propagation of tension disturbances to the given span. This has been achieved through online computation of correlation coefficient and reducing the interaction through fuzzy feedback control.     

Survey of Risk Management in Major U.K. Companies

Risk management consists of five stages, which are essential to the success of a major project. These five stages are identification, analysis, evaluation, response, and monitoring. The variations in using such risk management practices are considerable and are dependent on numerous factors such as the industry sector. This dependency is investigated and presented in this paper. Over 100 companies within the construction industry and oil and gas operators in the United Kingdom were surveyed through questionnaires that elicited information on the size and range of activities of the company, the techniques of risk analysis currently being used, the company's policy on responding to risk, as well as identifying specific risks encountered during any particular project. This paper highlights the vital conclusions accumulated from an analysis of the responses to the various sections of the questionnaire. The main conclusions from this paper are as follows: Personal and corporate experience and engineering judgment are the most successful qualitative techniques; expected monetary value, expected net present value, sensitivity analysis, and decision analysis are the principal quantitative techniques; and risk reduction (through staff training and education and the improvement of working conditions) is the most popular method of responding to risk.     

New techniques in the elastic analysis of arch bridges using image processing

The importance of the masonry arch in Britain's infrastructure has never been more significant than at the present time with ever increasing axle loads under new EEC regulations The searches have been shown to be significantly affected by the overlying fill. The effect of this fill is being investigated by a number of methods employing a variety of experimental techniques. This paper presents one such new technique of examining the effects of the fill through monitoring zones of strain on an arch model subjected to load. The model arches were constructed in timber with a dry granular fill and approximately 90 tests were carried out to investigate the effect of load position, fill depth and pier stiffness. Image analysis of the tests permitted identification of the areas of strain within the fill and arch barrel and the location of the hinges at higher loads. Finite element (FE) analysis was used to model the arch in its elastic range and comparisons of the FE strain contour plots with the equivalent images illustrated the validity of using image processing for the determination of the strain characteristics. The practical applications of using such a technique are subsequently highlighted and commented on.     

Web Tension regulation of multispan roll-to-roll system using integrated active dancer and load cells for printed electronics applications

The mass production of primed electronics can be achieved by roll-to-roll（R2R） printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of printed devices in limits. The web tension of a R2R system is regulated by the use of integrated load cells and active dancer system for printed electronics applications using decentralized multi-input-single-output（MISO） regularized variable learning rate backpropagation artificial neural networks. The active dancer system is used before printing system to reduce disturbances in the web tension of process span. The classical PID control result in tension spikes with the change in roll diameter of winder and unwinder rolls. The presence of dancer in R2R system shows that improved web tension control in printing span and the web tension can be enhanced from 3.75 N to 4.75 N. The overshoot of system is less than ±2.5 N and steady state error is within ± 1 N where load cells have a signal noise of ±0.7 N. The integration of load cells and active dancer with self-adapting neural network control provide a solution to the web tension control of multispan roll-to-roll system.     

Induced pressure pumping in polymer microchannels via field-effect flow control

Microfluidic field-effect flow control (FEFC) modifies the zeta potential of electroosmotic flow using a transverse electric field applied through the microchannel wall. Previously demonstrated in silicon-based and glass microsystems, FEFC is presented here as an elegant method for flow control in polymer-based microfluidics with a simple and low-cost fabrication process. In addition to direct FEFC flow modulation, independent transverse electric fields in connected microchannels are demonstrated to produce a differential pumping rate between the microchannels. The different electroosmotic pumping rates formed by local zeta potential control induce an internal pressure at the microchannel intersection, resulting in hydrodynamic pumping through an interconnecting field-free microchannel. Modulation of the voltages applied to the gate electrodes adjusts the magnitude and direction of the bidirectional pressure pumping, with fine resolution volume flow rates from -2 to 2 nL/min in the field-free microchannel demonstrated.