Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level

Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival.     

Note of Caution for the Aqueous Behaviour of Metal-Based Drug Candidates

Poor aqueous solubility is one of the recurrent drawbacks of many compounds in medicinal chemistry. To overcome this limitation, the dilution of drug candidates from stock solutions of an organic solvent is common practice. However, the precise characterisation of these compounds in aqueous solutions is often neglected, leading to some uncertainties regarding the nature of the actual active species. In this communication, we demonstrate that two ruthenium complexes previously reported by our group for their chemotherapeutic potential against cancer, namely [Ru(DIP)₂(sq)](PF₆) and [Ru(DIP)₂(3-methoxysq)](PF₆), where DIP is 4,7-diphenyl-1,10-phenanthroline, sq=semiquinonate and 3-methoxysq=3-methoxysemiquinonate, form colloids in water-DMSO (1 % v/v) mixtures that are invisible to the naked eyes. [Ru(DIP)₂(3-methoxysq)](PF₆) was found to form a highly stable and monodispersed colloid with nanoaggregates of ∼25 nm. In contrast, [Ru(DIP)₂(sq)](PF₆) was found to form large reticulates of mostly spherical aggregates which size was found to increase over time. The difference in size and shape distribution of drug candidates is of tremendous significance as the study of their biological activity might be severely affected. Overall, we strongly believe that these observations should be taken into account by the scientific community working on the development of metal-based drugs with poor water solubility.     

Matrix Transformation in Boron Containing High-Temperature Co–Re–Cr Alloys

An addition of boron largely increases the ductility in polycrystalline high-temperature Co–Re alloys. Therefore, the effect of boron on the alloy structural characteristics is of high importance for the stability of the matrix at operational temperatures. Volume fractions of ε (hexagonal close-packed—hcp), γ (face-centered cubic—fcc) and σ (Cr₂Re₃ type) phases were measured at ambient and high temperatures (up to 1500 °C) for a boron-containing Co–17Re–23Cr alloy using neutron diffraction. The matrix phase undergoes an allotropic transformation from ε to γ structure at high temperatures, similar to pure cobalt and to the previously investigated, more complex Co–17Re–23Cr–1.2Ta–2.6C alloy. It was determined in this study that the transformation temperature depends on the boron content (0–1000 wt. ppm). Nevertheless, the transformation temperature did not change monotonically with the increase in the boron content but reached a minimum at approximately 200 ppm of boron. A probable reason is the interplay between the amount of boron in the matrix and the amount of σ phase, which binds hcp-stabilizing elements (Cr and Re). Moreover, borides were identified in alloys with high boron content.     

Determination du coefficient d'extinction molaire de OH− dans les verres fluores a base de metaux lourds

The extinction coefficient ? at 2.9 μm for OH in fluoride glasses is measured by determining the amount of HF evolved during heating of the glass under steam and the corresponding intensity of the OH absorption band. This coefficient is respectively equal to 31 litre mole^?1 cm^?1 for BTYbZ glass (15 BaF₂ - 29 ThF₄ - 28 YbF₃ - 28 ZnF₂) and 19.5 litre mole^?1 cm^?1 for BALLA glass (34 BaF₂ - 57 ZrF₄ - 4 AlF₃ - 5 LaF₃).     

On coordinating warehouse sizing, leasing and inventory policy

Organizations often fulfil their storage needs by supplementing their own warehouse with leased space, a scenario modeled here under the assumption of constant product demand. Closed-form formulae are obtained for the decision variables of interest, namely, the replenishment lot size and the warehouse size, as well as the amount of space to lease. Cost savings due to leasing are shown to have an upper bound of approximately 29% when the optimal warehouse capacity can be installed without restrictions (such as a budgetary constraint). A numerical example further indicates that leasing is significantly more beneficial when the warehouse size is tightly constrained, and that total costs are robust with respect to demand fluctuations.     

Study of the degree of III conditioning of the phase space of pencil-beam radiotherapy using singular value decomposition

Dose distribution optimization algorithms are necessary in pencil-beam radiotherapy to exploit efficiently the multiple parameters of this powerful irradiation technique. We propose as an optimization technique singular value decomposition (SVD) analysis, which allows the measurement of ill conditioning of the stereotactic radiotherapy inverse problem and yields optimal weights for conformal treatment. Our approach to dose distribution optimization is to recover estimates of the minibeams weights from well-defined previsional dose matrices to study the influence of the different parameters on the stereotactic radiotherapy inverse procedure. The adjustment of the different parameters of the stereotactic irradiation to the “SVD optimizer” procedure is realized taking into account the ratio of the quality reconstruction to the calculation time. It will permit a more efficient use of the SVD optimizer in clinical applications with real three-dimensional lesions. We show the efficiency of the SVD optimizer in analyzing and predicting ill conditioning in stereotactic radiotherapy and in recognizing the topography of the different beams to create an optimal vector containing the beam weights (reconstructed weighting vector).     

Bizarre imagery, interference, and distinctiveness.

Previous studies have shown that bizarre and common images produce equivalent levels of recall in unmixed-list designs. Using unmixed lists, we tested the view that bizarre images would be less susceptible than common images to common sources of interference. In all experiments, subjects imaged a list of either bizarre or common sentences and then performed some kind of interfering task before recalling the initial list of sentences. Experiment 1 showed that bizarre images were better accessed than common images after imaging an intervening list of common sentences. Also, components of common images tended to be better recalled than those of bizarre images after imaging an intervening list of bizarre sentences. Experiments 2a and 2b showed that interfering tasks consisting of studying lists of common concrete nouns did not differentially affect memory for bizarre and common images. In Experiment 3, labeling and imaging an interfering list of common pictures produced higher recall of bizarre images. Generally, bizarre images appeared to be less susceptible than common images to interference from certain types of common encodings. Importantly, the superior recall of bizarre images was always due to greater image (sentence) access, whereas higher recall of common images was associated with greater recovery of the image (sentence) constituents. Explanation of the precise pattern of results requires consideration of the distinctive properties of bizarre images. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

基于SIP的新一代电信业务

第二代(2G)移动网络和传统的固定网络都提供了一套相对完整的话音业务。尽管智能网络(IN)技术使电信业务有所增强,但电信业务的发展仍然缓慢,而且样式基本保持不变。下一代网络(NGN)和第三代(3G)移动网络就是为了克服这些局限性而设计的,它们通过重组网络架构将业务同网络分离开来、将信息和电话技术融合起来并引进了会话初始协议(SIP)等开放协议。同时,它们还有望提供满足用户和运营商需求的新的增值业务。这些网络包括满足用户对业务期望所需的所有组成部分。NGN和3G就其服务能力而言有3个主要优点:首先,它们将信息世界和电话世界结…