Implant surface features as key role on cell behavior

It has been recognized that physical and chemical properties of biomaterial surfaces mediate the quality of extracellular matrix (ECM) that may affect cell behaviors. In nature, ECM is a heterogeneous three-dimensional superstructure formed by three major components, glycosaminoglycan, glycoconjugate, and protein, that anchors cellular compartments in tissues and regulates the function and the behavior of cells. Changes in the biointerface alter the quality of ECM and morphology through cell surface receptors, which, in turn, enable it to trigger specific cell signaling and different cellular responses. In fact, a number of strategies have been used to improve the functionality of surfaces and direct cell behavior through precisely designed environments. Herein, we aimed to discuss, through a science-based viewpoint, the biomaterial surface features on cell behavior and analyze the impact of cell physical modification on dental implant development.     

ALPHA-LINOLENIC ACID AND LINOLEIC ACID IN SERUM AND TISSUES AFTER FLAXSEED (Linum usitatissimum) OIL IN VIVO ADMINISTRATION

Essential fatty acids can be helpful in the prevention of several pathologies. The bioavailability of acute supplementation of different doses of flaxseed oil was studied by analyzing the level of alpha-linolenic acid (ALA) and linoleic acid (LA) in serum and tissues (adipose, liver) of rats tested at 2, 4, 8 and 16 h after the administration. The amount of flaxseed oil administered at increasing doses corresponded to 1, 2.5 and 5 g ALA/kg of body weight. The corresponding fatty acid methyl esters obtained via direct methylation were quantified using gas chromatography/mass spectrometry. Serum ALA level increased after 1 or 2.5 g/kg. ALA was increased in both adipose and liver tissue 4 h after the administration of 1 g/kg of flaxseed oil. There was no further increase by using a higher oil dosage. LA did not change in serum at the doses used.

PRACTICAL APPLICATIONS

The bioavailability of acute supplementation of increasing doses of flaxseed oil was examined by analyzing the level of alpha-linolenic acid (ALA) (ω3) and linoleic acid (ω6) in serum and tissues (adipose, liver).These data suggest that there is a limiting step in the absorption of these fatty acids and that there is no advantage to take more than 1 g/kg of ALA supplementation. The daily use of flaxseed oil could be a good alternative in some cases to fish oil in the prevention of several pathologies.     

SOLAR TUNNEL DRYING OF TURMERIC (CURCUMA LONGA LINN. SYN. C. DOMESTICA VAL.) FOR QUALITY IMPROVEMENT

Turmeric is one of the most ancient medicinal spices of the world. As its homeland, India acts as the largest producer, exporter and consumer of turmeric. The value of turmeric is due to its color and flavor, which is being given by curcumin, volatile oil and oleoresin. The end quality of turmeric is very much dependent on its post-harvest methods. Traditionally, open sun drying is the chief method adopted for processing. In the present investigation, freshly harvested turmeric rhizomes were collected from 30 stations and drying experiments were conducted by adopting three methods: (1) solar tunnel drying; (2) conventional drying; and (3) commercial drying. Various pre-drying and post-drying treatments were conducted. The results proved that conventional processing could maintain the intrinsic quality up to a certain level, but extrinsic quality could not be achieved. Solar tunnel drying method is an effective alternative to traditional open sun drying, where retention of curcumin, volatile oil and oleoresin was high, with less drying time. The study also disclosed the importance of pre-drying and post-drying treatments and hygienic practices to be adopted during processing.

PRACTICAL APPLICATIONS

Solar tunnel dryer can be used to process most of the spices, vegetables, fruits and other perishable crops with maximum retention of intrinsic quality and for better shelf life. Substantial reduction of drying time and physical quality improvement with regard to international quality standards are the other advantages.     

AN APPLICATION OF THE DISCRETE MAXIMUM PRINCIPLE TO THE SEGMENTATION OF A WALL FOR NONLINEAR SLOPES

A problem consisting of the optimal design of a segmented wall on a surface having a nonlinear slope contour is considered. It is formulated as an optimal control problem and a solution procedure based on the Discrete Maximum Principle is developed. An application to a real case, which previously had been solved by a Dynamic Programming based method, is included together with some critical comments on the above-mentioned technique.     

STORAGE RESULTS IN LOSS OF THE ANTIGENOTOXIC PROPERTIES OF LENTINULA EDODES (SHIITAKE MUSHROOM) AND DEVELOPMENT OF IN VIVO GENOTOXICITY

In this study, we have investigated the stability of the antigenotoxic properties of dried of Lentinula edodes (Berk.) Singer mushroom powder stored at room and at − 20C. Mice were pretreated by gavage for 15 consecutive days with 0.6 mL/day of an aqueous extract of dried L. edodes powder prepared at 60C. On day 15, the mice were intraperitoneally injected with 50 mg/kg N-ethyl-N-nitrosourea (ENU); 3 h later, peripheral blood was withdrawn from the animals and used to evaluate DNA damage using the comet assay. The mice were sacrificed 24 h after the ENU treatment and the bone marrow used to measure the frequency of micronucleated polychromatic erythrocytes. The results indicated that storage at both room temperature and at − 20C significantly reduced the antigenotoxic effects of the mushroom powder. In addition, whereas freshly dried mushroom powder was nongenotoxic, storage of the powder at − 20C resulted in genotoxic activity ( P <  0.05). Taken together, our data indicate that constituents of L. edodes powder are altered during storage, resulting in both genotoxicity and a reduction in antigenotoxicity.     

Controllability of linear single-input positive discrete-time systems

Linear positive systems are utilized in various scientific areas such as economic modelling, behavioural science, stochastic processes etc. A recent study has focused on some sufficient conditions for the controllability of these systems. A full characterization is provided of the controllability for discrete-time single-input positive systems. Necessary and sufficient conditions are derived using a digraph-theoretic approach. The main results show that strong constraints are to be imposed on the digraph associated with the pair (A, b) to ensure the controllability of the system.