Pull-Down of Metalloproteins in Their Native States by Using Desthiobiotin-Based Probes

One-third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active-site metals under mild conditions so as to prevent changes in metallation state. The proof-of-concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn²⁺-containing protein. CA was targeted by using sulfonamide derivatives. Linkers of various lengths and structures were screened to determine the optimal structure for capture of the native protein. The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull-down of differently metallated CAs was also investigated.     

渣油胶质和沥青质在分散型催化剂作用下的临氢热反应行为

采用四组分法从辽河减压渣油中分离出胶质、沥青质，分别考察了它们在分散型催化剂作用下的临氢热反应行为。结果表明，胶质、沥青质一方面要裂化生成较轻的产物；另一方面也要发生缩合反应生成较重的反应产物以及甲苯不溶物。渣油在临氢热反应过程中，沥青质是生焦的主要来源，其次才是胶质。比较了热反应生成的沥青质与原生沥青质之间以及热反应生成的胶质与原生胶质之间化学组成的区别，结果表明，反应生成的沥青质和胶质较原生的沥青质和胶质分子结构缩合程度高，并且反应苛刻度越高，缩合程度也越高。     

搪瓷釜的使用维护与常用修补方法

介绍搪瓷釜的使用维护要点，简单介绍目前国内外常用的搪瓷釜修补方法，从用、管、修相结合出发来延长搪瓷釜的使用寿命。     

孔板—均速管组合法测量汽—水两相流双参数

利用孔板与均速管组合在线测量汽—水两相流双参数，解决了稠油热采中同时测量蒸汽流量与干度的难题。其工作程序用Mcs-51单片机汇编语言编制而成，主程序由初始化、数据采集处理，热物理参数生成、汽—水两相流量、干度、质量流量、热量计算以及累计运算等模块组成。通过在现场进行标定；干度用化验CI^-法进行标定，流量用锅炉入口的涡轮流量计标定。经胜利和新疆两油田进行的大量试验可知，该仪表测量水蒸汽流量和干度其均方差皆小于7%，满足了工业应用要求。     

高压热处理对氧沉淀低温形核的影响

研究了外加高压(1GPa)下对450℃热处理硅片中氧沉淀行为的影响.透射电镜观察表明相对于大气压下热处理的样品,高压处理的样品会产生密度更高、尺寸更小的氧沉淀,表明高压有利于小直径氧沉淀的生成.电学性能测试表明,高压下处理的样品其热施主生成浓度和生成速率远远高于常压下处理的样品,这表明热施主与低温热处理过程中生成的高浓度氧沉淀核心有密切的关系.     

Irradiation Defects in Silicon Crystal

The application of irradiation in silicon crystal is introduced.The defects caused by irradiation are reviewed and some major ways of studying defects in irradiated silicon are summarized.Furthermore the problems in the investigation of irradiated silicon are discussed as well as its properties.     

悬浮床加氢裂化油溶性催化剂与沥青质的作用

在高压釜反应器中对添加了油溶性 Ni 催化剂(UPC-O)和水溶性 Ni 催化剂(UPC-W)的辽河稠油常压渣油(LHAR)进行了悬浮床加氢裂化实验。通过元素分析和傅里叶变换红外光谱(FTIR)分析对抑制反应生焦效果好的 UPC-O 与 LHAR 沥青质的作用进行了研究。元素分析结果显示,在 LHAR 中加入 UPC-O1000μg/g,沥青质和脱沥青质油中 Ni 含量分别出476μg/g和110μg/g 增加到7 559μg/g 和1 024μg/g,表明 UPC-O 在沥青质中有富集的趋势。FTIR 表征结果显示,UPC-O 和沥青质发生了相互作用。UPC-O 可在沥青质原位硫化为活性组分,对沥青质在悬浮床加氢裂化反应中的缩合起到好的抑制作用。     

Geotechnical properties of the soft soil in Guangzhou College City

The paper describes some geotechnical properties of the soft soils in Guangzhou College City, China, which are difficult to both sample and test. Laboratory and in situ tests were carried out to assess the physical and mechanical indices of the soils. The data were statistically analyzed and linear regression undertaken such that equations could be developed by which the geotechnical properties of the soft soils can be predicted. The statistical validity of the degree of correlation confirmed that using these equations, the mechanical indices can be estimated from physical indices determined by routine testing.      

柳钢新办公大楼智能管理系统的建设

介绍柳钢新办公大楼智能管理系统的建设设想、建设目的、系统基本功能。     

FTIR characterization of tropical wood–polymer composites

Wood–polymer composites (WPC) of Geronggang (Cratoxylon arborescens), a light tropical hardwood, impregnated with methyl methacrylate (MMA), methyl methacrylate-co-acrylonitrile (1 : 1; MAN), and styrene-co-acrylonitrile (3 : 2; STAN), were prepared by in situ polymerization using gamma radiation or the catalyst–heat treatment. The FTIR spectra of the three types of WPC, with polymer loadings ranging from 10 to 70%, were compared with that of the wood itself and the respective polymers. Characteristic peaks due to C?O vibration of MMA, C?N stretching of acrylonitrile, and ring stretching and bending of styrene monomers, were prominent in the samples that had higher polymer loadings. For the copolymeric systems, quantitation of the FTIR spectra of these characteristic peaks enabled calculations of incorporated acrylonitrile and styrene monomers in the composites to be made. The FTIR spectra of the residues remaining, after exhaustive extraction to remove homopolymer, showed that graft copolymerization of wood components with acrylonitrile and styrene monomers was possible, but not with MMA. Composites prepared by the two methods, gamma radiation and the catalyst–heat treatment, were shown to be chemically very similar.