Morphological evolution on the surface of hydrothermally synthesized hydroxyapatite microspheres in the presence of EDTMP

Well-ordered and surface engineered hierarchical hydroxyapatite microspheres (HAM) were prepared via a template free hydrothermal process. Ethylene diamine tetra (methylene phosphonic acid) (EDTMP) was used as chelating or regulating agent for the first time in this study. The results indicated the formation of sheet-like particles in the absence of EDTMP. On the other hand, microspheres with radially grown nanorods (HAMNR) or nanosheets (HAMNS) on the surface were obtained (with average diameter of 5?µm) in the presence of EDTMP. X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy were used to characterize the crystalline phases in the synthesized samples. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that EDTMP concentration played an important part in regulating the morphology to form well organized microspheres with nanosheets or nanorods on the surface. Brunauer-Emmett-Teller (BET) revealed an increase in the specific surface area with the change in morphology from the HAMNS to HAMNR. Possible mechanisms are proposed to account for the formation of different morphologies based upon thermodynamic and kinetic theories.     

Molecular follow-up of disease progression and interferon therapy in chronic myelocytic leukemia

We previously reported that the abl promoter (Pa) undergoes de novo DNA methylation in the course of chronic myelocytic leukemia (CML). The clinical implications of this finding are the subject of the present study in which samples of CML patients, including a group treated with interferon alpha (IFNalpha) were surveyed. The methylation status of the abl promoter was monitored by polymerase chain reaction (PCR) amplification of the Pa region after digestion with several site-methylation sensitive restriction enzymes. Some 74% of the DNA samples from blood and marrow drawn in the chronic phase were nonmethylated, similar to control samples from non-CML patients. The remaining 26% were partially methylated in the abl Pa region. The latter samples were derived from patients who were indistinguishable from the others on the basis of clinical presentation. Methylated samples were mostly derived from patients known to have a disease of longer duration (26 months v 7.5 months, P = .01). Samples of 30 IFNalpha-treated patients were sequentially analyzed in the course of treatment. Fifteen patients with no evidence of Pa methylation before treatment remained methylation-free. The remainder, who displayed Pa methylation before treatment, reverted to the methylation-free status. The outcome is attributed to IFNalpha therapy, as the Pa methylation status was not reversed in any of the patients treated with hydroxyurea. Methylation of the abl promoter indicates a disease of long-standing, most likely associated with a higher probability of imminent blastic transformation. It appears to predict the outcome of IFNalpha therapy far better than the cytogenetic response.     

Formal process algebraic modeling,verification, and analysis of an abstract Fuzzy Inference Cloud Service

In cloud computing, services play key roles. Services are well defined and autonomous components. Nowadays, the demand of using Fuzzy inference as a service is increasing in the domain of complex and critical systems. In such systems, along with the development of the software, the cost of detecting and fixing software defects increases. Therefore, using formal methods, which provide clear, concise, and mathematical interpretation of the system, is crucial for the design of these Fuzzy systems. To obtain this goal, we introduce the Fuzzy Inference Cloud Service (FICS) and propose a novel discipline for formal modeling of the FICS. The FICS provides the service of Fuzzy inference to the consumers. We also introduce four novel formal verification tests, which allow strict analysis of certain behavioral disciplines in the FICS as follows: (1) Internal consistency, which analyzes the service in a strict and delicate manner; (2) Deadlock freeness; (3) Divergence freeness; and (4) Goal reach ability. The four tests are discussed and the FICS is verified to ensure that it can pass all these tests.     

Petrophysical data prediction from seismic attributes using committee fuzzy inference system

This study presents an intelligent model based on fuzzy systems for making a quantitative formulation between seismic attributes and petrophysical data. The proposed methodology comprises two major steps. Firstly, the petrophysical data, including water saturation (S_w) and porosity, are predicted from seismic attributes using various fuzzy inference systems (FISs), including Sugeno (SFIS), Mamdani (MFIS) and Larsen (LFIS). Secondly, a committee fuzzy inference system (CFIS) is constructed using a hybrid genetic algorithms-pattern search (GA-PS) technique. The inputs of the CFIS model are the outputs and averages of the FIS petrophysical data. The methodology is illustrated using 3D seismic and petrophysical data of 11 wells of an Iranian offshore oil field in the Persian Gulf. The performance of the CFIS model is compared with a probabilistic neural network (PNN). The results show that the CFIS method performed better than neural network, the best individual fuzzy model and a simple averaging method.     

Multi-criteria decision making for assembly line balancing

Assembly line balancing often has significant impact on performance of manufacturing systems, and is usually a multiple-objective problem. Neither an algorithmic nor a procedural assembly line balancing methodology is usually effective in solving these problems. This article proposes a data envelopment analysis (DEA) approach to solve an assembly line balancing problem. A computer-aided assembly line balancing tool as Flexible Line Balancing software is used to generate a considerable number of solutions alternatives as well as to generate quantitative decision-making unit outputs. The quantitative performance measures were considered in this article. Then DEA was used to solve the multiple-objective assembly line balancing problem. An illustrative example shows the effectiveness of the proposed methodology.     

Bi-level fuzzy based advanced reservation of Cloud workflow applications on distributed Grid resources

The increasing demand on execution of large-scale Cloud workflow applications which need a robust and elastic computing infrastructure usually lead to the use of high-performance Grid computing clusters. As the owners of Cloud applications expect to fulfill the requested Quality of Services (QoS) by the Grid environment, an adaptive scheduling mechanism is needed which enables to distribute a large number of related tasks with different computational and communication demands on multi-cluster Grid computing environments. Addressing the problem of scheduling large-scale Cloud workflow applications onto multi-cluster Grid environment regarding the QoS constraints declared by application’s owner is the main contribution of this paper. Heterogeneity of resource types (service type) is one of the most important issues which significantly affect workflow scheduling in Grid environment. On the other hand, a Cloud application workflow is usually consisting of different tasks with the need for different resource types to complete which we call it heterogeneity in workflow. The main idea which forms the soul of all the algorithms and techniques introduced in this paper is to match the heterogeneity in Cloud application’s workflow to the heterogeneity in Grid clusters. To obtain this objective a new bi-level advanced reservation strategy is introduced, which is based upon the idea of first performing global scheduling and then conducting local scheduling. Global-scheduling is responsible to dynamically partition the received DAG into multiple sub-workflows that is realized by two collaborating algorithms: (1) The Critical Path Extraction algorithm (CPE) which proposes a new dynamic task overall critically value strategy based on DAG’s specification and requested resource type QoS status to determine the criticality of each task; and (2) The DAG Partitioning algorithm (DAGP) which introduces a novel dynamic score-based approach to extract sub-workflows based on critical paths by using a new Fuzzy Qualitative Value Calculation System to evaluate the environment. Local-scheduling is responsible for scheduling tasks on suitable resources by utilizing a new Multi-Criteria Advance Reservation algorithm (MCAR) which simultaneously meets high reliability and QoS expectations for scheduling distributed Cloud-base applications. We used the simulation to evaluate the performance of the proposed mechanism in comparison with four well-known approaches. The results show that the proposed algorithm outperforms other approaches in different QoS related terms.     

Non-Cytokine Protein Profile of the Mesenchymal Stem Cell Secretome That Regulates the Androgen Production Pathway

Polycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder in reproductive-aged women, and it typically involves elevated androgen levels. Recently, it has been reported that human bone marrow mesenchymal stem cells (hBM-MSCs) can regulate androgen synthesis pathways. However, the details of the mechanism are still unclear. hBM-MSC-derived secreted factors (the secretome) are promising sources of cell-based therapy as they consist of various types of proteins. It is thus important to know which proteins interact with disease-implicated biomolecules. This work aimed to investigate which secretome components contain the key factor that inhibits testosterone synthesis. In this study, we fractionated hBM-MSC-conditioned media into three fractions based on their molecular weights and found that, of the three fractions, one had the ability to inhibit the androgen-producing genes efficiently. We also analyzed the components of this fraction and established a protein profile of the hBM-MSC secretome, which was shown to inhibit androgen synthesis. Our study describes a set of protein components present in the hBM-MSC secretome that can be used therapeutically to treat PCOS by regulating androgen production for the first time.