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排序方式: 共有164条查询结果,搜索用时 31 毫秒
1.
Tuomas Sandholm 《Artificial Intelligence》2007,171(7):382-391
2.
Alfthan Kaija; Takkinen Kristiina; Sizmann Dorothea; Soderlund Hans; Teeri Tuula T. 《Protein engineering, design & selection : PEDS》1995,8(7):725-731
Single-chain antibodies were constructed using six differentlinker peptides to join the VH and VL domains of an anti-2-phenyloxazolone(Ox) antibody. Four of the linker peptides originated from theinterdomain linker region of the fungal cellulase CBHI and consistedof 28, 11, six and two amino acid residues. The two other linkerpeptides used were the (GGGGS)3 linker with 15 amino acid residuesand a modified IgG2b hinge peptide with 22 residues. Proteolyticstability and Ox binding properties of the six different scFvderivatives produced in Escherichia coli were investigated andcompared with those of the corresponding Fv fragment containingno joining peptide between the V domains. The hapten bindingproperties of different antibody fragments were studied by ELISAand BIAcoreTM. The interdomain linker peptide improved the haptenbinding properties of the antibody fragment when compared withFv fragment, but slightly increased its susceptibility to proteases.Single-chain antibodies with short CBHI linkers of 11, six andtwo residues had a tendency to form multimers which led to ahigher apparent affinity. The fragments with linkers longerthan 11 residues remained monomeric. 相似文献
3.
Mikko Nuutinen Toni Virtanen Tuomas Leisti Terhi Mustonen Jenni Radun Jukka Häkkinen 《Multimedia Tools and Applications》2016,75(4):2367-2391
The Dynamic Reference (DR) method has been developed for subjective image quality experiments in which original or undistorted images are unavailable. The DR method creates reference image series from test images. Reference images are presented to observers as a slide show prior to evaluating their quality. As the observers view the set of reference images, they determine the overall variation in quality within the set of test images. This study compared the performance of the DR method to that of the standardized absolute category rating (ACR) and paired comparison (PC) methods. We measured the performance of each method in terms of time effort and discriminability. The results showed that the DR method is faster than the PC method and more accurate than the ACR method. The DR method is especially suitable for experiments that require highly accurate results in a short time. 相似文献
4.
Dani Korpi Toni Heittola Timo Partala Antti Eronen Annamaria Mesaros Tuomas Virtanen 《Personal and Ubiquitous Computing》2013,17(4):761-769
When developing advanced location-based systems augmented with audio ambiances, it would be cost-effective to use a few representative samples from typical environments for describing a larger number of similar locations. The aim of this experiment was to study the human ability to discriminate audio ambiances recorded in similar locations of the same urban environment. A listening experiment consisting of material from three different environments and nine different locations was carried out with nineteen subjects to study the credibility of audio representations for certain environments which would diminish the need for collecting huge audio databases. The first goal was to study to what degree humans are able to recognize whether the recording has been made in an indicated location or in another similar location, when presented with the name of the place, location on a map, and the associated audio ambiance. The second goal was to study whether the ability to discriminate audio ambiances from different locations is affected by a visual cue, by presenting additional information in form of a photograph of the suggested location. The results indicate that audio ambiances from similar urban areas of the same city differ enough so that it is not acceptable to use a single recording as ambience to represent different yet similar locations. Including an image was found to increase the perceived credibility of all the audio samples in representing a certain location. The results suggest that developers of audio-augmented location-based systems should aim at using audio samples recorded on-site for each location in order to achieve a credible impression. 相似文献
5.
Hemminki Ari; Hoffren Anna-Marja; Takkinen Kristiina; Vehniainen Markus; Makinen Maija-Liisa; Pettersson Kim; Teleman Olle; Soderlund Hans; Teeri Tuula T. 《Protein engineering, design & selection : PEDS》1995,8(2):185-191
Europium chelates provide a non-radioactive alternative forsensitive labelling of antibodies for diagnostic immunoassays.Lysine residues at antibody surfaces are ready targets for labellingby an isothiocyanate derivative of the europium chelate (Eu3+).Here the labelling efficiency of a recombinant anti-human -fetoprotein(hAFP) Fab fragment has been improved by increasing its lysinecontent by protein engineering. Molecular modelling was usedto identify three light chain constant domain surface arginineresidues, R154, R187 and R210, which were mutated to lysineresidues. The mutations did not influence the affinity of thelysine-enriched Fab fragment and its labelling efficiency wasfound to be 40% higher than that of the wildtype Fab fragmentWith low degree of labelling, the affinities of the two Fabfragments were identical and comparable with that of the originalmonoclonal anti-hAFP IgG. With a higher degree of labellingthe affinities of both Fab fragments decreased more than thatof the intact IgG since more lysine residues are available forlabelling in the additional heavy chain constant domains ofthe larger molecule. Electrostatic adsorption and covalent immobilizationof the Fab fragments were characterized by BIAcoreTM and thelysine-enriched Fab fragment was found to be more efficientlyimmobilized to an activated carboxymethyl surface. 相似文献
6.
Tuomas Haggren Ali Shah Anton Autere Joona-Pekko Kakko Veer Dhaka Maria Kim Teppo Huhtio Zhipei Sun Harri Lipsanen 《Nano Research》2017,10(8):2657-2666
Light management and electrical isolation are essential for the majority of optoelectronic nanowire (NW) devices.Here,we present a cost-effective technique,based on vapor-phase deposition of parylene-C and subsequent annealing,that provides conformal encapsulation,anti-reflective coating,improved optical properties,and electrical insulation for GaAs nanowires.The process presented allows facile encapsulation and insulation that is suitable for any nanowire structure.In particular,the parylene-C encapsulation functions as an efficient antireflection coating for the nanowires,with reflectivity down to <1% in the visible spectrum.Furthermore,the parylene-C coating increases photoluminescence intensity,suggesting improved light guiding to the NWs.Finally,based on this process,a NW LED was fabricated,which showed good diode performance and a clear electroluminescence signal.We believe the process can expand the fabrication possibilities and improve the performance of optoelectronic nanowire devices. 相似文献
7.
Tuomas W. Sandholm 《Journal of Intelligent Manufacturing》2000,11(3):271-283
So far, most distributed scheduling systems have been designed for cooperative agents, and are inappropriate for self-interested agents, as for example in inter-firm interactions such as virtual enterprises. This paper discusses issues that arise in extending automated contracting to operate among such self-interested agents. We construct a leveled commitment contracting protocol that allows self-interested agents to efficiently accommodate future events by having the possibility of unilaterally decommitting from a contract based on local reasoning. A decommitment penalty is assigned to both agents in a contract to be freed from the contract, an agent only pays this penalty to the other party. It is formally shown that this leveled commitment feature in a contracting protocol increases Pareto efficiency of deals and can enable contracts by making them individually rational when no full commitment contract can. The analysis is nontrivial because self-interested agents decommit manipulatively a Nash equilibrium analysis of the decommitting game is necessary. 相似文献
8.
We consider an algorithmic problem that arises in the context of routing tables used by Internet routers. The Internet addressing scheme is hierarchical, where a group of hosts are identified by a prefix that is common to all the hosts in that group. Each host machine has a unique 32-bit address. Thus, all traffic between a source group s and a destination group d can be routed along a particular route c by maintaining a routing entry (s, d, c) at all intermediate routers, where s and d are binary bit strings. Many different routing tables can achieve the same routing behavior. In this paper we show how to compute the most compact routing table. In particular, we consider the following optimization problem: given a routing table D with N entries of the form (s, d, c) , determine a conflict-free routing table with fewest entries that has the same routing behavior as D. If the source and destination fields have up to w bits, and there are at most K different route values, then our algorithm runs in worst-case time O( NK w2) . 相似文献
9.
Hannon Erin E.; Snyder Joel S.; Eerola Tuomas; Krumhansl Carol L. 《Canadian Metallurgical Quarterly》2004,30(5):956
A number of different cues allow listeners to perceive musical meter. Three experiments examined effects of melodic and temporal accents on perceived meter in excerpts from folk songs scored in 6/8 or 3/4 meter. Participants matched excerpts with 1 of 2 metrical drum accompaniments. Melodic accents included contour change, melodic leaps, registral extreme, melodic repetition, and harmonic rhythm. Two experiments with isochronous melodies showed that contour change and melodic repetition predicted judgments. For longer melodies in the 2nd experiment, variables predicted judgments best at the beginning of excerpts. The final experiment, with rhythmically varied melodies, showed that temporal accents, tempo, and contour change were the strongest predictors of meter. The authors' findings suggest that listeners combine multiple melodic and temporal features to perceive musical meter. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
10.
Nataliya Strokina Jiri Matas Tuomas Eerola Lasse Lensu Heikki Kälviäinen 《Machine Vision and Applications》2016,27(3):387-396
The paper proposes a method for the detection of bubble-like transparent objects in a liquid. The detection problem is non-trivial since bubble appearance varies considerably due to different lighting conditions causing contrast reversal and multiple interreflections. We formulate the problem as the detection of concentric circular arrangements (CCA). The CCAs are recovered in a hypothesize-optimize-verify framework. The hypothesis generation is based on sampling from the partially linked components of the non-maximum suppressed responses of oriented ridge filters, and is followed by the CCA parameter estimation. Parameter optimization is carried out by minimizing a novel cost-function. The performance was tested on gas dispersion images of pulp suspension and oil dispersion images. The mean error of gas/oil volume estimation was used as a performance criterion due to the fact that the main goal of the applications driving the research was the bubble volume estimation. The method achieved 28 and 13 % of gas and oil volume estimation errors correspondingly outperforming the OpenCV Circular Hough Transform in both cases and the WaldBoost detector in gas volume estimation. 相似文献