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Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate
technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supernatant
of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence
reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin
culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography
sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50×104. Its maximum activity appears at pH 5 and 55 °C. The chitinase is stable at pH 4.0–7.5 and below 45 °C.
Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China 相似文献
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Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus niger CD-01 for pectin production 总被引:1,自引:1,他引:0
In order to solve the citrus peel resource waste problem and minimize the drawbacks of chemical extraction of pectin, a protopectinase-overproducing
strain CD-01 for pectin production was isolated from a pit soil dumped with perished orange in Changde City, Hunan Province
of China. The strain CD-01 had the same morphology and 28S rRNA gene sequence (FJ184995) as that of Aspergillus niger (ATCC 64028). It was thus identified and named as Aspergillus niger CD-01. The fermentation condition was optimized based on L9(34) orthogonal experimental design and the variances analyses. The results show that the optimal condition for producing pectin
is as follows: time 36 h, temperature 35 °C, pH 5, and urea as the nitrogen source. Under this condition, the pectin yield
can reach up to 24.5%. This shows a great potential of Aspergillus niger CD-01 in pectin extraction from citrus.
Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China 相似文献
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为解决甲骨文信息处理研究中对专家的依赖性高而专家知识共享率却很低这一突出矛盾,提出构建知识图谱的方案,其核心问题是如何发现实体和实体间的关联关系。基于甲骨文的古籍特征,先通过知识图谱MKD的共词分析发现实体及其关系,再利用甲骨文本体和规则推理发现隐含的语义关系,最后将其与MKD共词关系进行融合。实验结果表明,该方法可以有效解决甲骨文知识图谱中实体关系发现问题。 相似文献
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目的研究CPR期间给予肝素钠和小剂量高渗盐水治疗对复苏后兔小肠微循环灌注障碍的影响。方法 60只家兔开胸致室性颤动(室颤),室颤5 min后行胸内CPR,CPR过程中随机分为生理盐水(NS组)2 mL.kg-1.10 min-1或生理盐水2 mL.kg-1.10 min-1和普通肝素钠60 U.kg-1组(H组)或7.5%NaCl 2 mL.kg-1.10 min-1+普通肝素钠60 U.kg-1组(HSH组),每组20只。观察各组小肠灌注区域;室颤前及自主循环恢复(ROSC)后测血浆可溶性P选择素(sP)及内毒素(ET)浓度的变化情况。结果实验过程中各组之间的平均动脉压(MAP)差异无统计学意义(P〉0.05),兔经历5 min心脏骤停(CA)和CPR后,ROSC后30 min仍有严重的微循环灌注障碍,H组和NS组相比二者之间差异有统计学意义(P〈0.01);HSH组和其他2组相比差异均有统计学意义(P〈0.01)。ROSC后20、30 min HSH组血浆sP水平均明显低于其他2组(均P〈0.01)。各组之间ET基础值差异无统计学意义(P〉0.05),CA后随着自主循环的恢复,兔血浆ET浓度明显升高,并随着自主循环恢复时间延长有逐渐升高的趋势。ROSC后30、60、120 min,ROSC 3、4 h时HSH组、H组ET浓度与NS组比较差异均有统计学意义(均P〈0.01);而HSH组和H组之间差异无统计学意义(P〉0.05)。结论 CPR期间给予肝素钠和小剂量高渗盐水治疗可以改善复苏后兔小肠微循环灌注障碍,降低复苏后兔血浆内毒素浓度,并不增加出血的发生率。 相似文献
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为减少信息科学工作者在甲骨文数字化工作中对甲骨文专家的依赖,并有效解决甲骨文专家辨识和理解甲骨文的经验知识在知识共享及重用方面的问题,提出了构建甲骨文本体的方案。针对甲骨文的特点及其与现代汉语的内在联系,提出双向活动铰接法的甲骨文本体构建模型,参考7步法和知网的基本描述单位,系统分析了甲骨文本体的构建步骤。在甲骨文本体基础上,实现了优化信息检索及本体推理的应用。研究结果表明,甲骨文本体构建方法是可行的。 相似文献
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为在职业伤害发生之前,应用人机工程学的理论和方法,分析出伤害发生的原因,利用DELMIA软件,分析评估了高速列车装配过程中制动管安装工序存在的人机工程问题,并验证了DELMIA仿真评估的可靠性,对存在不良人机工效的作业进行了分析,并提出了改善措施,还在虚拟的装配环境下,检验了改善方案的合理性。 相似文献