Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens

The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually.     

EFFECTS OF SPECIFIED BLANK SIZE ON BODY WRINKLING DURING HYDRODYNAMIC DEEP DRAWING OF TAPERED RECTANGULAR BOX

1.~nonHydridapawhcdeepdrawing(HDD)belongstothefieldofsheetmetalfoeingandcanbeaPPliedinthefieldofautomotive,aerospace,drilitaryindustryetc.Ithasbeenrecognizedwidelyintheworld["'j.Fig.Ishowsitsprinciple:TheliqUidthatisfilledinthe~ngdiecavitywillbepressedwhenthepunchgoesdownsothattheeffectoffrictionkeepingandoverflowlubricatingcanbeformed,astherestilt,thefoeinglicitofsheetmetalwillbeimprovedareailvandthegualitVofthefinishedpartswillbeup~ed.BecauseofmanyadvantagesofHDDprocees,ithasbeenwidelys…     

铝-镁-硅系合金的挤压参数及机械性能

虽然铝-镁-硅系合金(AA6×××系)是最经常使用的挤压材料,其产量约占全世界挤压材的70％～80％,然而探讨此类合金挤压参数的文献却很少。所以本文的目的不是优选合金,而是研究铝-镁-硅系合金的技术应用范围。 1.坯锭生产连续铸造出圆锭,再锯成12个坯锭。坯锭的直径为123毫米,长度为500毫米。其中一半在铸态下挤压,另一半在580℃均匀化16小时(保温时间)随后空冷后挤     

DBST: a lightweight block cipher based on dynamic S-box

IoT devices have been widely used with the advent of 5G. These devices contain a large amount of private data during transmission. It is primely important for ensuring their security. Therefore, we proposed a lightweight block cipher based on dynamic S-box named DBST. It is introduced for devices with limited hardware resources and high throughput requirements. DBST is a 128-bit block cipher supporting 64-bit key, which is based on a new generalized Feistel variant structure. It retains the consistency and significantly boosts the diffusion of the traditional Feistel structure. The SubColumns of round function is implemented by combining bit-slice technology with subkeys. The S-box is dynamically associated with the key. It has been demonstrated that DBST has a good avalanche effect, low hardware area, and high throughput. Our S-box has been proven to have fewer differential features than RECTANGLE S-box. The security analysis of DBST reveals that it can against impossible differential attack, differential attack, linear attack, and other types of attacks.     

Measurement-based modeling of contact forces and textures for haptic rendering

Haptic texture represents the fine-grained attributes of an object's surface and is related to physical characteristics such as roughness and stiffness. We introduce an interactive and mobile scanning system for the acquisition and synthesis of haptic textures that consists of a visually tracked handheld touch probe. The most novel aspect of our work is an estimation method for the contact stiffness of an object based solely on the acceleration and forces measured during stroking of its surface with the handheld probe. We establish an experimental relationship between the estimated stiffness and the contact stiffness observed during compression. We also measure the height-displacement profile of an object's surface enabling us to generate haptic textures. We show an example of mapping the textures on to a coarse surface mesh obtained with an image-based technique, but the textures may also be combined with coarse surface meshes obtained by manual modeling.     

Spherical sample holders to improve the susceptibility measurement of superparamagnetic materials

The design of two custom sample holders with a spherical cavity for commercial vibrating sample magnetometer systems is described. For such cavities, the magnetization M[over ->] and the internal magnetic field H(i)[over ->] of a sample are both homogeneous. Consequently, the material parameter M(H(i)) of a sample can be determined even for liquids and powders with a high magnetic susceptibility.     

β-HEA对PMMA/SiO2纳米复合材料性能的影响

以丙烯酸-β-羟乙酯（β-HEA）为交联剂,采用溶胶-凝胶工艺制备了透明的PMMA/SiO2纳米复合材料,通过 IR、TMA、TGA和溶胶分数等研究了材料的结构和性能,结果表明复合材料中存在Si-O-C共价键,热稳定性明显优于有机玻璃,玻璃化转变温度为250 ℃以上,兼备有机玻璃和无机玻璃的性能优势.     

纺织助剂中的甲醛测定

基于更为严厉的甲醛重新分类的背景,TEGEWA协会整理工作组已开发出一种可确定纺织助剂中游离甲醛含量的新方法。目前此法鉴于其统一的程序,可使因不同的内部测试方法而得出不同观点的测试成为过去。