The effects of periodontal therapy on serum antibody levels to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis (part II)

Levels of IgG and IgM antibodies were estimated against Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were determined by enzyme linked immunosorbent assay (ELISA) in 17 patients with juvenile periodontitis, 15 with adult periodontitis and 24 healthy controls at the beginning of treatment and 3 to 8 months after periodontal therapy. After treatment, antibodies to A. actinomycetemcomitans and P.gingivalis had decreased in patients, but the levels were still significantly higher than in healthy controls. Whether or not an of antibody level against a specific bacteria changes after periodontal treatment is however, still debatable.     

Chromatographic performance of monodisperse–macroporous particles produced by “modified seeded polymerization.” I: Effect of monomer/seed latex ratio

In this study, the monodisperse–macroporous particles produced by a relatively new polymerization protocol, the so‐called, “modified seeded polymerization,” were used as column‐packing material in the reversed phase chromatography (RPC) of proteins. The particles were synthesized in the form of styrene‐divinylbenzene copolymer approximately 7.5 μm in size. In the first stage of the synthesis, the monodisperse polystyrene particles 4.4 μm in size were obtained by dispersion polymerization and used as the “seed latex.” The seed particles were swollen by a low‐molecular‐weight organic agent and then by a monomer mixture. The monodisperse–macroporous particles were obtained by the polymerization of monomer mixture in the seed particles. In the proposed polymerization protocol, the number of successive swelling stages was reduced with respect to the present techniques by the use of sufficiently large particles with an appropriate average molecular weight as the seed latex. A series of particles with different porosity properties was obtained by varying the monomer/seed latex ratio. The separation behavior of HPLC columns including the produced particles as packing material was investigated in the RPC mode using a protein mixture including albumin, lysozyme, cytochrome c, and ribonuclease A. The chromatograms were obtained with different flow rates under an acetonitrile–water gradient. The theoretical plate number increased and chromatograms with higher resolutions were obtained with the particles produced by using a lower monomer/seed latex ratio. The separation ability of the column could be protected over a wide range of flow rates (i.e., 0.5–3 mL/min) with most of the materials tested. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: 607–618, 2004     

Monodisperse‐porous poly(styrene‐co‐divinylbenzene) beads providing high column efficiency in reversed phase HPLC

A multistage polymerization protocol, the so‐called “modified seeded polymerization,” was developed for the production of monodisperse‐porous poly(styrene‐co‐divinylbenzene) providing high column efficiency as a packing material in reversed phase high performance liquid chromatography (RPLC). In the first stage of the multistage production, uniform polystyrene seed particles, produced by dispersion polymerization, were swollen by an organic agent (i.e., the diluent) and then by a monomer mixture containing styrene and divinylbenzene. The final porous particles were obtained in the monodisperse form by the polymerization of monomer mixture in the seed particles. By the use of a small size seed latex with low molecular weight and by the selection of the appropriate diluent, relatively small monodisperse‐porous particles with suitable pore structure could be achieved. In the reversed phase separation of alkylbenzenes, under isocratic conditions, theoretical plate numbers up to 40,000 plates/m were achieved by using 5.2 μm porous particles, obtained by a toluene‐dibutyl phthalate mixture as the diluent. No significant decrease in the resolution power was observed by the fourfold increase in the mobile phase flow rate. The column efficiency and the resolution observed with 5.2 μm monodisperse‐porous particles were significantly higher with respect to the currently available polymer based packing materials used in the reversed phase HPLC. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 95: 1430–1438, 2005     

Molecular mechanism of temporal physico/chemical changes that take place during imidization of polyamic acid: Coupled real-time rheo-optical and IR dichroism measurements

Multiple overlapping physical and chemical changes often take place during casting/drying and imidization from PMDA-ODA polyamic acid precursors from cast solutions. To shed light into details of these complex phenomena, we designed a unique real time measurement system that combines true stress, true strain, in-plane birefringence and temperature with polarized ultra-rapid scan FT-IR spectrometry (URS-FT-IR). At the early stages of heating (21°C–130 °C), initially isotropic solution cast film was observed to develop stress and birefringence as the solvent decomplexed and evaporated without showing any imidization as it was held in uniaxially constrained state. At a temperature around 130 °C, the onset of imidization reaction was detected while the stress went through a maximum. Beyond this stage, the evaporation of bound solvent and chemical conversion was observed to take place simultaneously and this is accompanied by a steady increase in birefringence. As the majority of the bound solvent evaporated, the stress and birefringence values started leveling off at long times.     

Bending and free vibration analysis of isotropic and multilayered plates and shells by using a new accurate higher-order shear deformation theory

Bending and free vibration analysis of multilayered plates and shells by using a new accurate higher order shear deformation theory (HSDT) is presented. It is one of the most accurate HSDT available in the literature, mainly because new non-polynomial shear strain shape functions (combination of exponential and trigonometric) used in the present theory are richer than polynomial functions, and free surface boundary conditions can be guaranteed a priori. The present HSDT is able to reproduce Touratier’s HSDT as special case. The governing equations and boundary conditions are derived by employing the principle of virtual work. These equations are then solved via Navier-type, closed form solutions. Bending and dynamic results are presented for cylindrical and spherical shells and plates for simply supported boundary conditions. Panels are subjected to sinusoidal, distributed and point loads. Results are provided for thick to thin as well as shallow and deep shells. The present results are compared with the exact three-dimensional elasticity theory and with several other well-known HSDT theories. The present HSDT is found to be more precise than other several existing ones for analyzing the bending and free vibration of isotropic and multilayered composite shell and plate structures.     

Column solid‐phase extraction of sunset yellow and spectrophotometric determination of its use in powdered beverage and confectionery products

A new and simple solid‐phase extraction method for the determination of trace levels of sunset yellow has been developed on Diaion HP 2MG column system. The adsorbed sunset yellow on the resin was eluted with 3 mL of methanol and determined by UV–Vis spectrophotometry at 484 nm. Various experimental parameters such as pH, amount of sunset yellow, flow rates of sample and eluent solutions, resin amount, sample volumes, etc. were investigated. The influences of some alkali, alkali earth and transition metals on the recoveries of sunset yellow were investigated. The recovery values for sunset yellow were higher than 95%. Preconcentration factor was found as 150. The detection limit (three sigma) of the reagent blank for sunset yellow was 5.2 μg L^?1. The relative standard deviations (RSD) of the procedure were found as 3.9%. The validation of the presented procedure was performed by the method of standard addition. The procedure was successfully applied to the determination of trace levels of sunset yellow in powdered beverage and confectionery products.     

Using Circular Conduits in Ozone Injection

Dissolved oxygen is the amount of oxygen dissolved in water corresponding to an important water quality parameter in rivers, streams, and lakes. Hydraulic structures can increase dissolved oxygen levels by creating turbulent conditions where small air bubbles are carried into the bulk of the flow. A gated conduit is a hydraulic structure that can be used efficiently in aeration and oxygen transfer. The subatmospheric pressure between the upstream and downstream of the gate is the reason for the air injection. Ozone is an unstable gas comprised of three oxygen atoms, and it can be used for water treatment. Ozone is thermodynamically unstable and spontaneously reverts back into oxygen. Ozone has been widely accepted as an effective disinfectant and a chemical oxidant. In this study a series of experiments was conducted to determine the ozone injection performance of circular conduits. Results showed that circular conduits are very effective for ozone injection.     

Exploiting Narrow Values for Soft Error Tolerance

Soft errors are an important challenge in contemporary microprocessors. Particle hits on the components of a processor are expected to create an increasing number of transient errors with each new microprocessor generation. In this paper we propose simple mechanisms that effectively reduce the vulnerability to soft errors In a processor. Our designs are generally motivated by the fact that many of the produced and consumed values in the processors are narrow and their upper order bits are meaningless. Soft errors canted by any particle strike to these higher order bits can be avoided by simply identifying these narrow values. Alternatively soft errors can be detected or corrected on the narrow values by replicating the vulnerable portion of the value inside the storage space provided for the upper order bits of these operands. We offer a variety of schemes that make use of narrow values and analyze their efficiency in reducing soft error vulnerability of level-1 data cache of the processor     

An Electrostatic Lens for Focusing Charged Particles in a Mass Spectrometer

The ability to transmit particles into the ablation region of an aerosol mass spectrometer determines in part the lower size limit for particles that can be analyzed. A large fraction of small particles (< 100 nm) are lost due to processes such as Brownian diffusion that broaden the particle beam. In this work, electrostatic focusing is used to overcome the limits of aerodynamic focusing in the analysis of nanometer-sized particles by aerosol mass spectrometry. A simple tube lens is used to focus charged particles into the ablation laser beam path. The diameter of the focused beam is smaller than the fundamental aerodynamic limit imposed by Brownian motion. Measured enhancements of the hit rate for particles between 21 and 33 nm diameter are between 3 and 6. These values are lower limits for the true enhancements. The lens is also energy selective and can be used to select the mass (size) of the particles being analyzed.     

System-level power-aware design techniques in real-time systems

Power and energy consumption has recently become an important issue and consequently, power-aware techniques are being devised at all levels of system design; from the circuit and device level, to the architectural, compiler, operating system, and networking layers. In this paper, we concentrate on power-aware design techniques for real-time systems. While the main focus is on hard real-time, soft real-time systems are considered as well. We start with the motivation for focusing on these systems and provide a brief discussion on power and energy objectives. We then follow with a survey of current research on a layer-by-layer basis. We conclude with illustrative examples and open research challenges. This paper provides an overview of power-aware techniques for the real-time system engineer as well as an up-to-date reference list for the researcher.