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排序方式: 共有191条查询结果,搜索用时 15 毫秒
1.
Shin-Ting W Yasuda CL Cendes F 《IEEE transactions on visualization and computer graphics》2012,18(2):299-308
Curvilinear reformatting of 3D magnetic resonance imaging data has been recognized by the medical community as a helpful noninvasive tool for displaying the cerebral anatomy. It consists of automatically creating, with respect to a reference surface, a series of equidistant curvilinear slices at progressively deeper cuts. In comparison with planar slices, it allows more precise localization of lesions and identification of subtle structural abnormalities. However, current curvilinear reformatting tools either rely on the time-consuming manual delineation of guiding curves on 2D slices, or require costly automatic brain segmentation procedures. In addition, they extract the skin and skull, impeding a precise topographic correlation between the location of the brain lesion and skin surface. This impairs planning of craniotomy for neurosurgery, and of the appropriate implantation of electrodes for intracranial electroencephalography in presurgical evaluation. In this work, we present a novel approach based on direct manipulation of the visualized volume data. By using a 3D painting metaphor, the reference surface can be defined incrementally, according to the principle that the user interacts with what she/he sees. As a response, an animation of the reformatting process is displayed. The focus of this paper is a new volume tagging algorithm behind user interactions. It works at an interactive frame rate on current graphics hardware. 相似文献
2.
Valeria Gaspari Camilla Ceccarani Marco Severgnini Gionathan Orioni Tania Camboni Luca Laghi Sara Morselli Claudio Foschi Antonella Marangoni Clarissa Consolandi Bianca Maria Piraccini 《International journal of molecular sciences》2022,23(10)
Background: Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic. Methods: Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., “CT-negative” (n = 21) and “CT-positive” (n = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by 1H-NMR. Results: Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by Lactobacillus, L. iners and L. crispatus being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family—in particular, of the Ureaplasma parvum species—was observed during CT infection. The Chlamydia genus was positively correlated with urine hippurate and lactulose. Conclusions: These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches. 相似文献
3.
Izadora de Souza Maria Carolina Clares Ramalho Camila Banca Guedes Isabeli Yumi Araújo Osawa Linda Karolynne Seregni Monteiro Luciana Rodrigues Gomes Clarissa Ribeiro Reily Rocha 《International journal of molecular sciences》2022,23(13)
Glioblastoma multiforme is a lethal disease and represents the most common and severe type of glioma. Drug resistance and the evasion of cell death are the main characteristics of its malignancy, leading to a high percentage of disease recurrence and the patients’ low survival rate. Exploiting the modulation of cell death mechanisms could be an important strategy to prevent tumor development and reverse the high mortality and morbidity rates in glioblastoma patients. Ferroptosis is a recently described type of cell death, which is characterized by iron accumulation, high levels of polyunsaturated fatty acid (PUFA)-containing phospholipids, and deficiency in lipid peroxidation repair. Several studies have demonstrated that ferroptosis has a potential role in cancer treatment and could be a promising approach for glioblastoma patients. Thus, here, we present an overview of the mechanisms of the iron-dependent cell death and summarize the current findings of ferroptosis modulation on glioblastoma including its non-canonical pathway. Moreover, we focused on new ferroptosis-inducing compounds for glioma treatment, and we highlight the key ferroptosis-related genes to glioma prognosis, which could be further explored. Thereby, understanding how to trigger ferroptosis in glioblastoma may provide promising pharmacological targets and indicate new therapeutic approaches to increase the survival of glioblastoma patients. 相似文献
4.
Samantha Baldassarri Daniela Benati Federica DAlessio Clarissa Patrizi Eleonora Cattin Michela Gentile Angelo Raggioli Alessandra Recchia 《International journal of molecular sciences》2022,23(14)
Sleeping Beauty (SB) is the first DNA transposon employed for efficient transposition in vertebrate cells, opening new applications for genetic engineering and gene therapies. A transposon-based gene delivery system holds the favourable features of non-viral vectors and an attractive safety profile. Here, we employed SB to engineer HEK293 cells for optimizing the production of a chimpanzee Adenovector (chAd) belonging to the Human Mastadenovirus C species. To date, chAd vectors are employed in several clinical settings for infectious diseases, last but not least COVID-19. A robust, efficient and quick viral vector production could advance the clinical application of chAd vectors. To this aim, we firstly swapped the hAd5 E1 with chAd-C E1 gene by using the CRISPR/Cas9 system. We demonstrated that in the absence of human Ad5 E1, chimp Ad-C E1 gene did not support HEK293 survival. To improve chAd-C vector production, we engineered HEK293 cells to stably express the chAd-C precursor terminal protein (ch.pTP), which plays a crucial role in chimpanzee Adenoviral DNA replication. The results indicate that exogenous ch.pTP expression significantly ameliorate the packaging and amplification of recombinant chAd-C vectors thus, the engineered HEK293ch.pTP cells could represent a superior packaging cell line for the production of these vectors. 相似文献
5.
Marleen Julia Meyer Simon Falk Sarah Rmer Clarissa Prinzinger Sabine Tacke Joachim Geyer Stefan Simm Mladen Vassilev Tzvetkov 《International journal of molecular sciences》2022,23(9)
OCT1 and OCT2 are polyspecific membrane transporters that are involved in hepatic and renal drug clearance in humans and mice. In this study, we cloned dog OCT1 and OCT2 and compared their function to the human and mouse orthologs. We used liver and kidney RNA to clone dog OCT1 and OCT2. The cloned and the publicly available RNA-Seq sequences differed from the annotated exon-intron structure of OCT1 in the dog genome CanFam3.1. An additional exon between exons 2 and 3 was identified and confirmed by sequencing in six additional dog breeds. Next, dog OCT1 and OCT2 were stably overexpressed in HEK293 cells and the transport kinetics of five drugs were analyzed. We observed strong differences in the transport kinetics between dog and human orthologs. Dog OCT1 transported fenoterol with 12.9-fold higher capacity but 14.3-fold lower affinity (higher KM) than human OCT1. Human OCT1 transported ipratropium with 5.2-fold higher capacity but 8.4-fold lower affinity than dog OCT1. Compared to human OCT2, dog OCT2 showed 10-fold lower transport of fenoterol and butylscopolamine. In conclusion, the functional characterization of dog OCT1 and OCT2 reported here may have implications when using dogs as pre-clinical models as well as for drug therapy in dogs. 相似文献
6.
7.
Luciana F. Fleuri Paula K. Novelli Clarissa H. O. Delgado Mayara R. Pivetta Milene S. Pereira Mariana de L. C. Arcuri Bruna L. Capoville 《International Journal of Food Science & Technology》2014,49(12):2585-2591
Lipase from Aspergillus sp. obtained by solid‐state fermentation (SSF) on wheat bran (LWB), soybean bran (LSB) and soybean bran combined with sugarcane bagasse (LSBBC) were 67.5, 58 and 57.3 U of crude lipase per gram substrate, respectively. The optimum pH of activity and stability of the LWB was between 8 and 9, and the optimum temperature of activity and stability was 50 °C and up to 60 °C, respectively. The LSB and LSBBC showed two peaks of optimum pH (4 and 6) and optimal values of temperature and stability at 50 °C. The LSB was stable in the pH range of 6–7, while LSBBC in the range of pH 4–7. All the enzymes show activities on p‐nitrophenyl esters (butyrate, laurate and palmitate). LWB stood out either on the hydrolysis of sunflower oil, presenting 66.1% of the activity over commercial lipase and on the esterification of oleic acid and ethanol, surpassing the activities of the commercial lipases studied. The thin layer chromatography showed that LWB and LSB have produced ethyl esters from corn oil, while LWB produced it from sunflower oil. 相似文献
8.
Luciana Francisco Fleuri Mariana Cassani de Oliveira Mariana de Lara Campos Arcuri Bruna Luiza Capoville Milene Stefani Pereira Clarissa Hamaio Okino Delgado Paula Kern Novelli 《Food science and biotechnology》2014,23(4):1199-1205
Fungal strains were screened for lipase producing activities and 10 strains were classified as good producers. Aspergillus sp., Fusarium sp., and Penicillium sp. exhibited the highest activities when fermented in wheat bran (WB) and soybean bran (SB). No fungal growth was observed using sugarcane bagasse (CB). An experimental design was applied to incorporate CB into the fermentation process for lipase production by Aspergillus sp. and Penicillium sp., and to evaluate the best moisture content for the substrate. Strains studied achieved maximum lipase activities with 25% CB combined with 75% WB or SB at 40% moisture content. The highest lipase activities were observed for WB and SB, and for SB combined with CB using Aspergillus sp. Fermentation of 96 h was the optimum period for enzyme production. 相似文献
9.
Chloromethane is an important reagent for methylations in the process industry. However, as a gas suspected of causing cancer, it is rarely used at laboratory scale. Therefore, a setup is presented here for studies in a laboratory under safe and reproducible conditions. The use of a microreactor guarantees high heat transfer rates and a low holdup of the reagent. As a proof-of-concept, the reaction of chloromethane with the secondary amine morpholine in aqueous solution is investigated. By applying elevated pressures, a liquid-liquid system with enhanced solubility of chloromethane in the aqueous phase is accessible. 相似文献
10.
Valesca Cristiane Benelli Francisco Clarissa Hamaio Okino-Delgado Mirella R. Zanutto Elgui Célio Junior da Costa Fernandes Roselaine Facanali Rodrigo Augusto da Silva Willian Fernando Zambuzzi Márcia Ortiz Mayo Marques Luciana Francisco Fleuri 《International Journal of Food Science & Technology》2019,54(4):1089-1099
The aim of this study was to show that abundant and inexpensive plant oils can be biotransformed to increase biological activity (antioxidant, antimicrobial and cytotoxicity) through hydrolysis reaction catalysed by lipases. We tested homemade and commercial lipases through the biotransformation of nine different plant oils in forty different combinations. First, the chemical composition of the samples was investigated. Thereafter, biological tests were conducted to assess the antioxidant and antimicrobial activity of the sampled biotransformation products, as well as analyzed their influence on the viability of healthy and cancer cells. Summarising, sunflower, corn and olive oils modified by orange waste-obtained lipases presented the most promising results, reaching up to 90% of antioxidant activity increase and significant growth inhibition of bacteria colonies belonging to genera Escherichia, Listeria, Staphylococcus, Pseudomonas and Salmonella. In addition, those compounds affected human oral squamous carcinoma cells. The bioconversion of plant oils through lipases improves their biological properties and might be an option for biotechnological application. 相似文献