Effects of chloroquine treatment on antioxidant enzymes in rat liver and kidney

The effect of chloroquine (CHQ) administration on antioxidant enzymes in rat liver and kidney was studied. Male Sprague-Dawley rats were administered 20 mg/kg CHQ once a week for 4 weeks (chronic treatment) or a single dose at 10 or 20 mg/kg (acute treatment). Antioxidant enzyme activities were determined in cytosolic fractions of liver and kidney, whereas reduced glutathione (GSH) and malondialdehyde (MDA) were determined in tissue samples. Results indicate minimal effects of acute CHQ treatment, whereas chronic treatment with CHQ differentially affected antioxidant enzymes in the two organs. Superoxide dismutase activity was increased nearly twofold, while activities of selenium glutathione peroxidase (GPX), catalase, and NAD (P) H: quinone oxidoreductase were decreased in livers of CHQ-treated rats compared to controls. No significant effects of CHQ on glutathione reductase, GSH, and MDA levels were seen in the liver. Fewer effects of CHQ were observed in the kidney where a decrease in GPX activity and an increase in MDA levels was seen. Lowering of antioxidant enzymes activities in the liver by CHQ could render the organ more susceptible to subsequent oxidative stress; while increased MDA production after CHQ treatment in the kidney indicate that the organ is being subjected to oxidative stress. This could have implications for prolonged chloroquine intake.     

Construction experiences with SSC collider dipole magnets atFermilab

Full length and short model SSC (Superconducting Super Collider) 50 mm bore dipoles are being built and tested at Fermilab. The mechanical design of these magnets has been determined from experience with the construction of previous superconducting magnets. Construction experience includes coil fabrication, ground insulation, instrumentation, collaring, and yoke assembly. Fabrication techniques are explained, and construction problems and their solutions are reviewed. The relationship of short to long model construction is discussed     

Limited evidence of zebra mussel (Dreissena polymorpha) consumption by freshwater drum (Aplodinotus grunniens) in Lake Winnipeg

Freshwater drum (Aplodinotus grunniens) may be a predator of the invasive zebra mussel (Dreissena polymorpha), which established in Lake Winnipeg in 2013. In this study, the diets, trophic position, and growth of 51 freshwater drum collected in 2019 (six years post-zebra mussel invasion) were compared to 64 freshwater drum sampled in 2000. Benthic insect larvae were the dominant food items in both years. Although mollusks occur in high densities in Lake Winnipeg, they were only consumed by a few freshwater drum in either sample year. Zebra mussels were not a frequent prey item in 2019 as they were only consumed by four of the sampled freshwater drum. Stable isotope analysis of white muscle tissue yielded similar δ¹³C and δ¹⁵N values in both years and were consistent with a benthic, insectivorous diet. Length-at-age data derived from otoliths revealed that the 2019 population had at least an equal growth rate to the 2000 population. Weight-at-length data suggested that fish condition was greater in 2019 than in 2000, which coincided with increased benthic macroinvertebrate density in Lake Winnipeg. Based on these findings, Lake Winnipeg freshwater drum continue to feed predominantly on insect larvae and not zebra mussels.     

Fiber Coating Concepts for Brittle-Matrix Composites

The current interest in tough, high-temperature materials has motivated fiber coating development for brittle-matrix composites with brittle reinforcements. Such coatings are needed for controlled interface debonding and frictional sliding. The system investigated in this study was sapphire fiber-reinforced alumina. This system is thermochemically stable for severe use conditions, exhibits little thermal expansion mismatch, and utilizes the excellent strength and creep resistance of sapphire reinforcements. Porous oxide and refractory metal coatings which satisfy requirements for toughness improvement in these composites were identified by employing a variety of newly developed mechanical testing techniques for determining the interfacial fracture energies and sliding resistances.     

Experiences with large-scale operational trials of ALTO-enhanced P2P filesharing in an intra-ISP scenario

Application Layer Traffic Optimization (ALTO) has recently gained attention in the research and standardisation community as a way for a network operator to guide the peer selection process of distributed applications by providing network layer topology information. In particular P2P applications are expected to gain from ALTO, due to the many connections peers form among each other, often without taking network layer topology information into account. In this paper, we present results of an extensive intra-ISP trial with an ALTO-enhanced P2P filesharing software. In summary, our results show that—depending on the concrete setting and on the distribution of upload capacity in the network—ALTO enables an ISP to save operational costs significantly while not degrading application layer performance noticeably. In addition, based on our experience we are able to give advice to operators on how to save costs with ALTO while not sacrificing application layer performance at all.     

Data access in distributed simulations of multi-agent systems

Distributed simulation has emerged as an important instrument for studying large-scale complex systems. Such systems inherently consist of a large number of components, which operate in a large shared state space interacting with it in highly dynamic and unpredictable ways. Optimising access to the shared state space is crucial for achieving efficient simulation executions. Data accesses may take two forms: locating data according to a set of attribute value ranges (range query) or locating a particular state variable from the given identifier (ID query and update). This paper proposes two alternative routing approaches, namely the address-based approach, which locates data according to their address information, and the range-based approach, whose operation is based on looking up attribute value range information along the paths to the destinations. The two algorithms are discussed and analysed in the context of PDES-MAS, a framework for the distributed simulation of multi-agent systems, which uses a hierarchical infrastructure to manage the shared state space. The paper introduces a generic meta-simulation framework which is used to perform a quantitative comparative analysis of the proposed algorithms under various circumstances.     

Determination of fat in olestra-containing savory snack products by capillary gas chromatography

A quantitative method to determine fat in olestra-containing savory snack products was validated within the AOAC Peer-Verified Methods Program. The method may be used to demonstrate compliance with the guidelines of the U.S. Nutrition Labeling and Education Act for labeling products as "fat free" or "low fat." The method can measure total and saturated fat in savory snacks when present at levels of 0.2-10 g total fat and 0.1-3 g saturated fat per 30 g serving. The method is standardized to measure C6-C24 fatty acids. Extraction of olestra-containing savory snack samples with chloroform-methanol (modified AOAC Official Method 983.23) yields a lipid extract containing the total fat and olestra. The extracted lipid is hydrolyzed by lipase, yielding fatty acids and unreacted olestra. The fatty acids are precipitated as calcium soaps. Olestra is extracted from insoluble soaps with hexane and then discarded. The isolated soaps are converted back into fatty acids with hydrochloric acid and extracted with hexane. The isolated fatty acids are converted to methyl esters with boron trifluoride-methanol and quantitated by capillary gas chromatography using internal standard. Test samples were prepared by blending olestra-containing and full-fat (triglyceride) snacks to obtain 6 levels of spiking (0-10 g total fat added/30 g serving) in potato chips, potato crisps, cheese puffs, and nacho cheese-flavored corn chips. Results were linear (r2 > 0.997) between 0 and 10 g fat/30 g serving for each product matrix. Mean recovery was 101 +/- 6% standard deviation (SD) for total fat and 104 +/- 6% SD for saturated fat. Mean recovery by peer laboratory was 88 +/- 5% SD for total fat and 95 +/- 4% SD for saturated fat in potato chips (0-3 g total fat added/30 g serving). Two sets of 10 replicates of potato chips (0.5 g total fat/30 g serving and 0.16 g saturated fat/30 g serving) and potato crisps (0.5 g total fat/30 g serving and 0.16 g saturated fat/30 g serving) were analyzed by submitting and peer laboratories. Repeatability relative standard deviations ranged from 3.90 to 7.33% for total fat and from 4.01 to 11.53% for saturated fat. Reproducibility relative standard deviations were 7.33% (total fat, potato chips), 7.15% (total fat, potato crisps), 11.36% (saturated fat, potato chips), and 13.50% (saturated fat, potato crisps).     

Estimating body shape of dressed humans

The paper presents a method to estimate the detailed 3D body shape of a person even if heavy or loose clothing is worn. The approach is based on a space of human shapes, learned from a large database of registered body scans. Together with this database we use as input a 3D scan or model of the person wearing clothes and apply a fitting method, based on ICP (iterated closest point) registration and Laplacian mesh deformation. The statistical model of human body shapes enforces that the model stays within the space of human shapes. The method therefore allows us to compute the most likely shape and pose of the subject, even if it is heavily occluded or body parts are not visible. Several experiments demonstrate the applicability and accuracy of our approach to recover occluded or missing body parts from 3D laser scans.     

A Review of Critical Plane Orientations in Multiaxial Fatigue Failure Criteria of Metallic Materials

The paper presents a review of multiaxial fatigue failure criteria based on the critical plane concept. The criteria have been divided into three groups, according to the fatigue damage parameter used in the criterion, i.e. (i) stress, (ii) strain and (iii) strain energy density criteria. Each criterion was described mainly by the critical plane orientation. Multiaxial fatigue criteria based on the critical plane concept usually apply different loading parameters in the critical plane whose orientation is determined by (a) only shear loading parameters (crack Mode II or III), (b) only normal loading parameters (crack Mode I) or sometimes (c) mixed loading parameters (mixed crack Mode). There are also criteria based on few critical plane orientations and criteria based on critical plane orientations determined by a weighted averaging process of rotating principal stress axes.