2005年日本爱知县世博会西班牙馆

2005年日本爱知县世博会中西班牙馆的设计主题是反映西班牙发展历程中的文化融合。     

深圳宝安机场

建筑师在深圳国际机场的方案中提出建造一座功能优化且节约能源的建筑,并打造其成为深圳市的重要地标之一。     

里奥哈技术转化中心

里奥哈技术转化中心位于里奥哈大区的中心城市Logrono，在不失各自特色和共享功能的前提下,将多个研究中心整合在一座建筑中，如国家IT教育中心，IT技术转化中心和IT商业孵化中心。     

Torrevieja市立歌剧院和报告厅

Torrevieja镇是西班牙东南部的一个重要旅游小镇．目前是一个大众旅游胜地。为提升城镇形象,政府正在酝酿一项新建城市基础设施的计划,该项目就是其中的一部分。     

东南海滨公园和礼堂

巴塞罗那是2004年国际文化论坛的主办城市,东南海滨公园和中庭项目属于该城市基础设施建设的一部分。FOA计划通过处理地形，将两个露天礼堂与海岸茂密的植被分开。     

第8届威尼斯建筑双年展英国馆

2002年，英国领事馆委托外事办公室建筑师设计一个与实地情况相符的英国馆参加威尼斯国际建筑双年展，鉴于城市主义，建筑和设计等学科面临生产方式变革式的改变以及经济一体化的发展，建筑师将面临很多棘手的问题。     

横滨国际码头

横滨国际码头是一种新型的城市便利交通设旋。设计方案提出将建筑设计成码头景观的继续和延伸,而不是一个脱离码头基址肌理的物体，在满足码头所有功能的同时还在其屋顶建造一个大型的城市公园。     

Meydan零售综合体

项目位于伊斯坦布尔发展最快的地区之一，Meydan零售中心是一个高效率的零售综合体，它也切实成为该地区的中心。该综合体位于伊斯坦布尔亚洲区的城郊结合部，毗邻宜家和一个即将动工的住宅区。     

Application of recording and processing of energy-filtered image sequences for the elemental mapping of biological specimens: Imaging-Spectrum

Computerized energy-filtered transmission electron microscope (EFTEM) permits the recording and the processing of energy-filtered images, allowing a part of an electron energy-loss spectrum for each picture element to be obtained. This method, called ‘Imaging-Spectrum’, uses a Zeiss CEM902 coupled to several image analysis systems. The actual configuration records sequences of 48 images, 256 × 256 pixels, in steps of the energy loss, ΔE. Processing these sequences results in part of a core-loss EELS-spectrum for each pixel. This approach produces elemental maps with a short processing time. We have implemented three kinds of background calculation for the image subtraction. The influence of the irradiation dose and of the energy selecting slit width on the quality of the spectra is investigated. The method is applied to the analysis of some biological specimens (pericellular coat behaviour during adhesion between macrophages and red blood cells and location of calcite microcrystals in dental pulp cells). The Imaging-Spectrum method appears to be suitable for the analysis of large areas.     

Contribution of energy-filtering TEM to the detection of calcium: Application to mast cells

The ultrastructural distribution and quantification of calcium in mast cells prepared by anhydrous processing was investigated by energy-filtering transmission electron microscopy (EFTEM) using a Zeiss 902 electron microscope. Optimal conditions for calcium detection were determined using inorganic (calcium phosphate) and organic (calcium-loaded chelex beads) standards with known amounts of calcium. Electron energy-loss spectroscopy (EELS) revealed calcium at the L_2,3 edge and also at the M_2,3 edge for all specimens examined. Comparison with X-ray microanalysis confirmed the results obtained with EELS. Electron spectroscopic imaging (ESI) was applied for mapping calcium both in standards and in cells and we showed that mast cell granules were the main site of calcium localization. Although, results have shown that a combination of analytical techniques is required to obtain reliable results.