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1.
Jay Ronel V. Conejos Jalil Ghassemi Nejad Jung-Eun Kim Jun-Ok Moon Jae-Sung Lee Hong-Gu Lee 《International journal of molecular sciences》2021,22(5)
The objective of this study was to investigate the effects of supplementing with L-tryptophan (L-Trp) on milk protein synthesis using an immortalized bovine mammary epithelial (MAC-T) cell line. Cells were treated with 0, 0.3, 0.6, 0.9, 1.2, and 1.5 mM of supplemental L-Trp, and the most efficient time for protein synthesis was determined by measuring cell, medium, and total protein at 0, 24, 48, 72, and 96 h. Time and dose tests showed that the 48 h incubation time and a 0.9 mM dose of L-Trp were the optimal values. The mechanism of milk protein synthesis was elucidated through proteomic analysis to identify the metabolic pathway involved. When L-Trp was supplemented, extracellular protein (medium protein) reached its peak at 48 h, whereas intracellular cell protein reached its peak at 96 h with all L-Trp doses. β-casein mRNA gene expression and genes related to milk protein synthesis, such as mammalian target of rapamycin (mTOR) and ribosomal protein 6 (RPS6) genes, were also stimulated (p < 0.05). Overall, there were 51 upregulated and 59 downregulated proteins, many of which are involved in protein synthesis. The results of protein pathway analysis showed that L-Trp stimulated glycolysis, the pentose phosphate pathway, and ATP synthesis, which are pathways involved in energy metabolism. Together, these results demonstrate that L-Trp supplementation, particularly at 0.9 mM, is an effective stimulus in β-casein synthesis by stimulating genes, proteins, and pathways related to protein and energy metabolism. 相似文献
2.
Hossein Tehrani Nik Nejad Nobuhiro Sugimura Koji Iwamura Yoshitaka Tanimizu 《Journal of Intelligent Manufacturing》2010,21(4):487-499
Due to rapid changes of markets and pressures of competitions, industries are adopting their production ways to support diversity
of customer’s needs and increase of new product developments. This paper deals with development of an agent-based architecture
of dynamic systems for process planning in the manufacturing systems. In consideration of alternative manufacturing processes
and machine tools, the process plans and the schedules of the manufacturing resources are generated incrementally and dynamically.
A previously proposed negotiation protocol is customized and improved to generate suitable process plans for the target products
real-timely and dynamically, based on the alternative manufacturing processes. The alternative manufacturing processes are
presented by the process plan networks and the suitable process plans are searched and generated to cope with both the dynamic
status and disturbances of the manufacturing systems. We initiatively combine the heuristic search algorithms of the process
plan networks with the negotiation protocols, in order to generate suitable process plans. 相似文献
3.
M. Jalaal M.G. Nejad P. Jalili M. Esmaeilpour H. Bararnia E. Ghasemi Soheil Soleimani D.D. Ganji S.M. Moghimi 《Computers & Mathematics with Applications》2011,61(8):2267-2270
He’s homotopy perturbation method is applied to obtain exact analytical solutions for the motion of a spherical particle in a plane couette flow. It is demonstrated that the applied analytical method is very straightforward in comparison with existing techniques. Furthermore, it is decidedly effectual in terms of accuracy and rapid convergence. The formulation of the problem is presented in the text as well as the analytical and numerical procedures. The current results can be used in different areas of particulate flows. 相似文献
4.
A single specimen test using the three point single edge notched beam configuration at low temperatures for obtaining hot mix asphalt (HMA) resistance curves is developed.Resistance curves are obtained for mixtures at six temperature levels of+5,0,-5,-10,-15,and-20 ℃ and three binder contents of 4%,4.5%,and 5%.Crack extension increments during the test are measured by means of an image processing technique using Radon transform and feature extraction.All the specimens exhibit a rising R-curve,indicating ductility and toughening mechanisms in the ductile-quasi brittle fracture of the mixture.It is observed that the reduction of temperature results in a further tendency of the mixture for unstable crack growth and less subcritical crack length.It is also shown that using the binarization process,an automatic index can be developed that can represent the extent of brittleness and extent of the low temperature in which the cracking has occurred. 相似文献
5.
Millets are generally dehulled and subjected to a hydrothermal treatment before consumption, thus the hulls can be used as a potential source of antioxidants. Several millet grains, namely kodo, finger (Ravi), finger (local), proso, foxtail, little and pearl millet were studied. Antioxidant activities of phenolic extracts obtained from whole grains, as well as their corresponding dehulled and cooked grains and hulls were studied for their total phenolic content (TPC), radical scavenging capacity, and antioxidant activity in a β-carotene/linoleate emulsion. The phenolics present in whole grains were identified and quantified using HPLC and HPLC/MS and results were expressed as total for each of the phenolic groups. The TPC ranged from 2 to 112 μmol ferulic acid equivalents/g defatted meal. All varieties exhibited effective inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, peroxyl and superoxide radicals. Dehulling and cooking affected the TPC and radical scavenging and antioxidant activities of the grains, depending on the variety. In general, the antioxidant activity of phenolic extracts was in the order of hull > whole grain > dehulled grain > cooked dehulled grain With the exception of the two finger millet varieties, hulls of other millet grains had high TPC, thus demonstrating their superior antioxidant activity. Hydroxybenzoic acids, hydroxycinnamic acids and flavonoids in whole grains were identified as contributors to the observed effects. Therefore, dehulling of grain and hydrothermal treatments affect the phenolic content and antioxidant potential of millet grains. 相似文献
6.
Vilailak Klompong Soottawat Benjakul Duangporn Kantachote Fereidoon Shahidi 《Food and Bioprocess Technology》2012,5(4):1317-1327
The objective of this study was to investigate the potential use of protein hydrolysate from yellow stripe trevally as a nitrogen source for the growth of different microorganisms. Protein hydrolysates from yellow stripe trevally with different degrees of hydrolysis (5, 15 and 25%) prepared using Alcalase (HA) or Flavourzyme (HF) were determined in comparison with commercial Bacto Peptone. For bacteria, Staphylococcus aureus and Escherichia coli, HF with 25% DH (HF25) yielded the highest cell density and specific growth rate (μ max) and the lowest generation time (t d) (p?0.05). For yeasts, Saccharomyces cerevisiae and Candida albicans, Bacto Peptone yielded the higher growth rate than did HA and HF (p?0.05), whereas no differences in μ max and t d were observed for fungus, Aspergillus oryzae (p?>?0.05). The pH of culture broth containing HF25 decreased markedly during the first 8 hours of cultivation of S. aureus and E. coli (p?0.05). This directly lowered the colony size of S. aureus (p?0.05). However, buffered culture broth containing HF25 rendered the similar growth and colony size of S. aureus (p?>?0.05), compared with that containing Bacto Peptone. Scanning electron microscopic study revealed no differences in size and shape of microorganisms cultured in HF25 and Bacto Peptone (p?>?0.05). 相似文献
7.
Ao Li Man-Tong Zhao Fa-Wen Yin Min Zhang Hui-Lin Liu Da-Yong Zhou Fereidoon Shahidi 《International Journal of Food Science & Technology》2021,56(6):2938-2945
The antioxidant effects of gallic acid (GA) and its alkyl esters including methyl gallate, ethyl gallate, propyl gallate, butyl gallate (GA-C4), octyl gallate, lauryl gallate, hexadecyl gallate and octadecyl gallate in frying oil (soybean oil) and oyster during frying were investigated. Rancimat induction period (RIP) assay indicated GA and its alkyl esters effectively improved the oxidative stability of soybean oil according to the ‘polar paradox’; that is, the antioxidant effects of the polyphenols decreased in lipid system with the increasing of hydrophobicity. Results also indicated that GA and its alkyl esters all effectively retarded lipid oxidation in oyster tissue according to parameters including RIP, peroxide value and electron spin resonance assays. Moreover, the antioxidant efficiency in oyster increased with the alkyl chain length until GA-C4, and thereafter, the antioxidant effects decreased, similar to the ‘cut-off effect’. This study provides basic data for improving the oxidative stability of seafood during frying. 相似文献
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10.
The present article solves the couple equations of a spherical solid particle’s motion in plane coquette fluid flow by using the HPM-Padé technique which is a combination of the Homotopy Perturbation Method (HPM) and Padé approximation. The series solutions of the couple equations are developed. Generally, the truncated series solution is adequately in a small region and to overcome this limitation, the Padé techniques which have the advantage of turning the polynomial approximation into a rational function, are applied to the series solution to improve the accuracy and enlarge the convergence domain. The current results compared with those derived from HPM and the established fourth order Runge–Kutta method in order to ascertain the accuracy of the proposed method. It is found that this method can achieve more suitable results in comparison to HPM. 相似文献