A new class of tobacco chitinases homologous to bacterial exo-chitinases displays antifungal activity

A novel chitinase gene of tobacco was isolated and characterized by DNA sequence analysis of a genomic clone and a cDNA clone. Comparative sequence analysis of both clones showed an identity of 94%. The proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes I-IV and are designated as class V chitinases. Comparison of the chitinase class V peptide sequence with sequences in the Swiss Protein databank revealed significant sequence similarity with bacterial exo-chitinases from Bacillus circulans, Serratia marcescens and Streptomyces plicatus. It was demonstrated that class V chitinase gene expression is induced after treatment of tobacco with different forms of stress, like TMV-infection, ethylene treatment, wounding or ultraviolet irradiation. Two related chitinase class V proteins of 41 and 43 kDa were purified from Samsun NN tobacco leaves inoculated with tobacco mosaic virus. The proteins were purified by Chelating Superose chromatography and gel filtration. In vitro assays demonstrated that class V chitinases have endo-chitinase activity and exhibit antifungal activity toward Trichoderma viride and Alternaria radicina. In addition, it was shown that class V chitinase acts synergistically with tobacco class I beta-1,3-glucanase against Fusarium solani germlings.     

The Inventory Approach to Urban Growth Boundaries

In this article, we argue that insights can be gained by analyzing the use of urban growth boundaries (UGBs) for urban growth management as an inventory problem. The analysis provides three useful insights. First, it shows that where UGBs are employed, too much emphasis is placed on whether UGBs contain sufficient land to accommodate 20 years of growth and too little is placed on how frequently, how much, or under what circumstances UGBs should be expanded. Second, it shows that where market factors are used, they have been used inappropriately to increase the size of UGBs immediately after expansion, when they should be used to influence the size of UGBs immediately before expansion. Third, it shows that UGBs are likely to work better if expansions occur not after an arbitrary period of time, but when the supply—or the price—of land reaches some critical threshold. We conclude by asserting that the use of UGBs should not be governed by arbitrary rules of thumb, because such rules do not stand up to critical examination, they preclude adaptation to local situations, and they prevent experimentation.     

Exotic introductions to the fishery of Lake Victoria: What are the management options?

Lake Victoria is Africa's most important source of inland fishery production, exhibiting annual catches of ≈ 400 000 mt. The predatory Nile perch, Lates niloticus, and the herbivorous tilapiines, Oreochromis niloticus, Oreochromis leucostictus, Tilapia zillii and Tilapia rendalii, were introduced in Lake Victoria in the 1950s and 1960s. Nile perch were introduced to convert the abundant, but bony, haplochromines to fish flesh, while the tilapiines were introduced to boost the declining fishery. Since that time, the fisheries of Lake Victoria have undergone dramatic social and ecological changes. The catches increased tremendously, changing the fishery from artisanal to commercial, in turn increasing fisher income and employment opportunities. However, there was a decline and, in some cases, the disappearance of many indigenous fish species, especially the haplochromine cichlids. This reduction was attributed to overexploitation, predation, and competition and hybridization with the introduced species. The decline of the native fish species has had impacts on the trophic and ecological status of the lake. Nile perch now dominate the formerly complex food web. The loss of phytoplanktivorous haplochromines has contributed to an increase in algal blooms, reduction in water quality and occasional fish kills. Water hyacinth, Eichhornia crassipes, invaded Lake Victoria in 1988, with high rates of infestation in shallow waters and bays, which are breeding and nursery grounds for most fish species. Catches of Nile perch decreased following the infestation, while those of Nile tilapia, lung fish (Protopterus aethiopicus) and mud fish (Clarias gariepinus) increased. Haplochromines species also showed some signs of recovery. In view of all these changes, the future of the Lake Victoria fishery is uncertain.     

Congenital nephrotic syndrome: a novel phenotype of type I carbohydrate-deficient glycoprotein syndrome

Type I carbohydrate-deficient glycoprotein (CDG) syndrome is a genetic multisystem disorder generally without overt renal problems. We report a neonate with neurological abnormalities and congenital nephrotic syndrome of diffuse mesangial sclerosis type. Serum transferrin isoelectric focusing showed the typical abnormalities of type I CDG syndrome. Normal transferrin focusing findings in other patients with similar renal problems excluded the possibility of a secondary biochemical phenomenon. The diagnosis of type I CDG syndrome was confirmed by demonstration of a deficiency of phosphomannomutase. No evidence of pontocerebellar atrophy was found in imaging or at autopsy. We conclude that congenital nephrotic syndrome may occur in type I CDG syndrome, and that this diagnosis should be considered in patients with congenital nephrotic syndrome. Absence of pontocerebellar atrophy does not exclude the diagnosis of type I CDG syndrome.     

Magnetic resonance imaging in lactic acidosis

Mitochondrial defects, defects in gluconeogenesis, and biotin-responsive multiple carboxylase deficiency are disorders characterized by primary lactic acidosis. In this review, characteristic findings in magnetic resonance imaging (MRI) of the brain, as related to histopathological abnormalities, are described for the different disorders and the diagnostic value of the MRI findings is discussed. Inborn errors of metabolism with primary lactic acidosis should be considered in particular when MRI shows lesions similar to or reminiscent of effects of focal or generalized hypoxia-ischaemia, or when MRI shows signs of chronic neurodegeneration, but rarely in cases with predominantly white-matter changes.     

Economic Basis for Linking Jobs and Housing in San Francisco

Although many have written about urban sprawl, few have sought to measure it. In this article, we present several quantitative measures of urban form and compute these for neighborhoods of varying age in Washington County, the western portion of the Portland, Oregon, metropolitan area. Our results suggest (1) neighborhoods in Washington County have increased in single-family dwelling unit density since the 1960s; (2) internal street connectivity and pedestrian access to commercial areas and bus stops have improved since the early 1990s; (3) external connectivity continues to decline; and (4) the mixing of land uses remains limited. We conclude that while several measures appear to be improving, Portland's war on sprawl is not yet won.     

Reduction of ferric ions in dilute solution in a semi-technical scale GBC reactor

The performance of a semitechnical gas diffusion electrode packed bed electrode cell (GBC) for the reduction of metal ions is investigated. Reduction of Fe3+ in sulphuric acid solution is described by first order kinetics and is found to be significantly influenced by temperature, H2SO4 concentration and the inlet concentration of Fe3+. The condition of packing has a major influence on the overall conversion in the cell. It is shown that a slight decrease in the amount of bed particles can cause a significant drop in the overall degree of conversion. The mass transfer coefficient km is found to be proportional to us0.48 at low flow velocities and us0.80 at high flow velocities, where us is the mean flow velocity. Partitioning of the overall reaction rate, ko,av, into an overall mass transfer coefficient km,av and an apparent overall electrochemical rate constant, kr,av, shows that, for H2SO4 concentration less than 1M, kr,av, can be described by an empirical relationship involving the effects of the inlet Fe3+ concentration, the solution conductivity and temperature.