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1.
Jens Mller Anne Busch Christian Berens Helmut Hotzel Andreas Burkovski 《International journal of molecular sciences》2021,22(7)
Corynebacterium silvaticum is a newly identified animal pathogen of forest animals such as roe deer and wild boars. The species is closely related to the emerging human pathogen Corynebacterium ulcerans and the widely distributed animal pathogen Corynebacterium pseudotuberculosis. In this study, Corynebacterium silvaticum strain W25 was characterized with respect to its interaction with human cell lines. Microscopy, measurement of transepithelial electric resistance and cytotoxicity assays revealed detrimental effects of C. silvaticum to different human epithelial cell lines and to an invertebrate animal model, Galleria mellonella larvae, comparable to diphtheria toxin-secreting C. ulcerans. Furthermore, the results obtained may indicate a considerable zoonotic potential of this newly identified species. 相似文献
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Prof. Dr.-Ing. Thorsten Spitta Dipl. Ing. Helmut Schmidpeter 《WIRTSCHAFTSINFORMATIK》2002,44(2):141-150
This paper analyses the characteristics of software houses as service providers. The tasks of internal IT-services are determined from these characteristics and a reporting system for the IT-controlling is outlined. The concept results from the analysis of one of the 25 largest German software houses. The paper concludes with a case study of the fiscal year 2000 IT-controlling report of the above-mentioned firm. 相似文献
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Heinz Blenke Beate Farys-Kahle Carl-Max Freiherr Von Meysenbug Helmut Weimer 《化学,工程师,技术》1985,57(5):381-390
Rationalizing drawings for chemical apparatus . For small and medium-sized manufacturers of apparatus, producing drawings may constitute a disproportionate waste of time and personnel, particularly when no new designs are involved but only modification or improvement of drawings supplied by the customer. One possibility for rationalizing drawing is to simplify the old-fashioned approach by using symbols for frequently drawn or standard items, or by listing alphanumerically. This presumes a kind of systematization permitting determination of those symbols and their reasonable use, as well facilitating rationalization by means of data processing. There are different approaches to computer-aided drawing (from computer controlled drawing-board to interactive screens); here a CAD programme is presented which was especially developed for graphic presentation of chemical apparatus, and which can be used without learning a programming language. 相似文献
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Is there a direct relationship between oral astringency and human salivary protein binding? 总被引:1,自引:0,他引:1
For decades, it is believed that astringency is due to the polyphenol-induced complexation of proline-rich salivary proteins
in the oral cavity. In order to compare for the first time the human sensory threshold concentrations and the salivary protein
binding activity of a series of astringent stimuli, human saliva protein was incubated for 5 min at 37 °C in the presence
of astringent food-derived compounds and, after micro-centrifugation, the amount of the target molecules in the supernatant
was quantitatively determined by HPLC-UV/Vis. Significant protein binding was observed for (−)-epigallocatechin-3-gallate,
(−)-gallocatechin-3-gallate, (+)-gallocatechin, and (−)-catechin-3-gallate, all of which containing at least one galloyl moiety
in the molecule and exhibiting rather high sensory thresholds of more than 200 μmol/L. In comparison, (+)-catechin and procyanidin
B2, both lacking in any galloyl function, showed only comparatively low binding activity and, most interestingly, quercetin-3-O-α-l-rhamnopyranosyl-(1 → 6)-β-d-glucopyranoside and 3-carboxymethyl-indole-1-N-β-d-glucopyranoside did not show any protein binding at all, although the later N- and O-glycosides exhibited extraordinarily low sensory threshold concentrations of less than 0.001 and 0.0003 μmol/L, respectively.
The data give some first evidence that the quantity of the non-bound, “free” astringent stimulus in the saliva liquid might
be more closely related to the sensory perception of astringency than the amount complexed or precipitated by proteins. It
is therefore questionable as to whether oral perception of astringency is related to the complexation and/or precipitation
of salivary proteins. 相似文献
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