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Kendra L. Van Buren François M. Hemez 《International journal for numerical methods in engineering》2016,105(5):351-371
This work proposes a method for statistical effect screening to identify design parameters of a numerical simulation that are influential to performance while simultaneously being robust to epistemic uncertainty introduced by calibration variables. Design parameters are controlled by the analyst, but the optimal design is often uncertain, while calibration variables are introduced by modeling choices. We argue that uncertainty introduced by design parameters and calibration variables should be treated differently, despite potential interactions between the two sets. Herein, a robustness criterion is embedded in our effect screening to guarantee the influence of design parameters, irrespective of values used for calibration variables. The Morris screening method is utilized to explore the design space, while robustness to uncertainty is quantified in the context of info‐gap decision theory. The proposed method is applied to the National Aeronautics and Space Administration Multidisciplinary Uncertainty Quantification Challenge Problem, which is a black‐box code for aeronautic flight guidance that requires 35 input parameters. The application demonstrates that a large number of variables can be handled without formulating simplifying assumptions about the potential coupling between calibration variables and design parameters. Because of the computational efficiency of the Morris screening method, we conclude that the analysis can be applied to even larger‐dimensional problems. (Approved for unlimited, public release on October 9, 2013, LA‐UR‐13‐27839, Unclassified.) Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
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In the past few years, the project management (PM) profession has grown exponentially. Yet recent studies in the information technology (IT) sector have found that the use of PM methodologies alone does not guarantee project success. In fact, IT project success is seen to rely on the ability of project managers to be agents of change (i.e., individuals who lead organizational change efforts), a traditional role of practitioners of organization development and change. This article identifies the knowledge, skills, and competencies that are common to organization development (OD) practitioners and project managers, as represented by 6 principles or competencies that OD and PM change methods snare: communication, teamwork, process management, leadership, training, and continuous learning. The importance of these 6 principles in developing professional project managers as effective agents of change is explored with the intent of finding means to improve upon current IT project success rates. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Fat-reduced cheeses often suffer from undesirable texture, flavor, and cooking properties. Exopolysaccharides (EPS) produced by starter strains have been proposed as a mechanism to increase yield and to improve the texture and cooking properties of reduced-fat cheeses. The objective of this work was to assess the influence of an exopolysaccharide on the yield, texture, cooking properties, and quality of half-fat Cheddar cheese. Two pilot-scale half-fat Cheddar cheeses were manufactured using single starters of an isogenic strain of Lactococcus lactis ssp. cremoris (DPC6532 and DPC6533) that differed in their ability to produce exopolysaccharide. Consequently, any differences detected between the cheeses were attributed to the presence of the exopolysaccharide. The results indicated that cheeses made with the exopolysaccharide-producing starter had an 8.17% increase in actual cheese yield (per 100 kg of milk), a 9.49% increase in moisture content, increase in water activity and water desorption rate at relative humidities ≤90%, significant differences in the cheeses microstructure, and a significant improvement in both textural and cooking properties, without negatively affecting the flavor profiles of the cheeses. 相似文献
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Simon W. So Kendra M. Fleming Cayla M. Duffy Joshua P. Nixon David A. Bernlohr Tammy A. Butterick 《International journal of molecular sciences》2022,23(8)
The microglial fatty-acid-binding protein 4-uncoupling protein 2 (FABP4-UCP2) axis is a key regulator of neuroinflammation in high-fat-diet (HFD)-fed animals, indicating a role for FABP4 in brain immune response. We hypothesized that the FABP4-UCP2 axis is involved in regulating diet-induced cognitive decline. We tested cognitive function in mice lacking microglial FABP4 (AKO mice). Fifteen-week-old male AKO and wild-type (WT) mice were maintained on 60% HFD or normal chow (NC) for 12 weeks. Body composition was measured using EchoMRI. Locomotor activity, working memory, and spatial memory were assessed using behavioral tests (open field, T-maze, and Barnes maze, respectively). Hippocampal microgliosis was assessed via immunohistochemical staining. An inflammatory cytokine panel was assayed using hippocampal tissue. Real-time RT-PCR was performed to measure microglial UCP2 mRNA expression. Our data support that loss of FABP4 prevents cognitive decline in vivo. HFD-fed WT mice exhibited impaired long- and short-term memory, in contrast with HFD-fed AKO mice. HFD-fed WT mice had an increase in hippocampal inflammatory cytokine expression (IFNγ, IL-1β, IL-5, IL-6, KC/GRO(CXCL1), IL-10, and TNFα) and microgliosis, and decreased microglial UCP2 expression. HFD-fed AKO mice had decreased hippocampal inflammatory cytokine expression and microgliosis and increased microglial UCP2 expression compared to HFD-fed WT mice. Collectively, our work supports the idea that the FABP4-UCP2 axis represents a potential therapeutic target in preventing diet-induced cognitive decline. 相似文献
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De Angelis M Curtin AC McSweeney PL Faccia M Gobbetti M 《The Journal of dairy research》2002,69(2):255-267
A homo-tetrameric approximately 160-kDa cystathionine gamma-lyase was purified to homogeneity from Lactobacillus reuteri DSM 20016 by four chromatographic steps. The activity was pyridoxal-5'-phosphate dependent and the enzyme catalyzed the alpha,gamma-elimination reaction of L-cystathionine, producing L-cysteine, ammonia and alpha-ketobutyrate. The enzyme was active towards a range of amino acids and amino acid derivatives, including methionine. The pH and temperature optima were found to be 8.0 and 35 degrees C. respectively. Isoelectric pH (pI) was approximately 5.0 as determined by two-dimensional electrophoresis. Sensitivity to chemical inhibitors was typical of lactococcal cystathionine gamma- and beta-lyases, except it was inhibited by sulphydryl reagents. The N-terminal sequence was MKFNTQLIHGGNSED, which had 100% homology with cystathionine beta-lyase of Lb. reuteri 104R (Accession Number (CAC05298). Lb. reuteri DSM 20016. together with 10 other strains of non-starter lactic acid bacteria, was used as adjunct starter in the production of miniature ('anestrato Pugliese-like cheeses. After 40 d ripening, the water-soluble extract of the cheeses with added Lactobacillus fermentum DT41 and Lb. reuteri DSM 20016 contained the highest enzyme activities on cystathionine and methionine substrates. Determinations of methanethiol, dimethyl sulphide, dimethyl disulphide and dimethyl trisulphide in the miniature cheeses confirmed the findings of enzyme activities. 相似文献
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Siefert Caleb J.; Kehl-Fie Kendra; Blais Mark A.; Chriki Lyvia 《Canadian Metallurgical Quarterly》2007,19(4):469
The present studies focus on strategies for detecting back irrelevant responding (BIR) on the Personality Assessment Inventory (PAI; L. C. Morey, 1991). Moderate BIR levels can greatly affect the clinical scales of the PAI. Further, the PAI's Inconsistency and Infrequency validity scales are less than optimal for detecting BIR. L. C. Morey and C. J. Hopwood (2004) developed an alternative strategy for detecting BIR that involves comparison of 2 scales from the PAI short-form with the same 2 scales from the PAI full-instrument. The present study examines how different BIR levels affect the clinical, treatment, and interpersonal scales of the PAI in 2 psychiatric inpatient samples. The effectiveness of various strategies for detecting BIR in an inpatient setting is also discussed. Consistent with previous research, moderate rates of BIR impacted several PAI scales in a meaningful way. The Inconsistency and Infrequency validity scales of the PAI were relatively ineffective for detecting low-to-moderate BIR levels. Conversely, the short-form full-instrument comparison strategy was much more sensitive to BIR. Finally, a new BIR detection indicator is presented that improves sensitivity rates for detecting all BIR levels in an acute setting. The implications of these results for detecting BIR in inpatient settings are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Antonio Pino Francisco Prados Elena Galán Paul L.H. McSweeney José Fernández-Salguero 《Food research international (Ottawa, Ont.)》2009,42(3):324-330
Powdered plant coagulant (PPC) obtained from the cardoon (Cynara cardunculus) was compared with calf rennet (CR) for the manufacture of goats’ milk cheese, by determining difference in the proteolysis throughout ripening. There were no substantial differences between the compositions of cheeses made using the two types of coagulants. However, cheeses manufactured with PPC exhibited higher levels of pH 4.6-SN than cheese made using CR. The extent of breakdown of αs-casein, as measured by urea-PAGE, was greater in cheese made using PPC than cheese made using CR. The formation of hydrophobic peptides and the ratio of hydrophobic/hydrophilic peptides throughout the ripening were higher in cheeses made with PPC than in cheeses made with CR. Principal component analysis (PCA) of peak heights of RP-HPLC peptide profiles of the ethanol-soluble and ethanol-insoluble fractions distributed the samples according to the coagulant used in their manufacture. Quantitative differences in several peptides were evident between the two types of cheese. 相似文献