Stormwater quality associated with a silt trap (empty and full) discharging into an urban watercourse in Scotland

The aim was to assess the influence of a full silt trap at the end of a stormwater drainage pipe on the water quality of stormwater discharged into a semi‐natural urban watercourse. For approximately eleven weeks, the water qualities of the preliminarily treated stormwater and of the receiving watercourse (Braid Burn) were studied. The mean outflow concentrations of suspended solids were 2.0 mg/l and 34.1 mg/l during dry and wet weather conditions, respectively. Suspended solids concentrations of up to 141.6 mg/l were recorded during storm events. Suspended solids values for treated stormwater were often too high compared to international secondary wastewater treatment standards of around 30 mg/l. Pollutants including heavy metals (e.g., zinc, copper and nickel) accumulated in the silt trap. However, high outflow velocities during heavy rainfall events did not result in clearly defined sediment layers due to sediment re‐suspension. Metals did not accumulate in the receiving watercourse.     

Fruit acid extracts, a fresh approach to skin renewal

Alpha-hydroxy acids are a common class of naturally occurring chemicals that have become increasingly popular for cosmetic use. Currently alpha-hydroxy acids from both natural and synthetic sources are being used in skincare products. From lactic acid to complex natural blends, several different approaches have been adopted for the use of alpha-hydroxy acids. This paper reviews the history of alpha-hydroxy acids use in cosmetics and gives new data showing the increased rate of cell renewal by topical application.     

Tracking protein dynamics with photoconvertible Dendra2 on spinning disk confocal systems

Understanding the dynamic properties of cellular proteins in live cells and in real time is essential to delineate their function. In this context, we introduce the Fluorescence Recovery After Photobleaching‐Photoactivation unit (Andor) combined with the Nikon Eclipse Ti E Spinning Disk (Andor) confocal microscope as an advantageous and robust platform to exploit the properties of the Dendra2 photoconvertible fluorescent protein (Evrogen) and analyse protein subcellular trafficking in living cells. A major advantage of the spinning disk confocal is the rapid acquisition speed, enabling high temporal resolution of cellular processes. Furthermore, photoconversion and imaging are less invasive on the spinning disk confocal as the cell exposition to illumination power is reduced, thereby minimizing photobleaching and increasing cell viability. We have tested this commercially available platform using experimental settings adapted to track the migration of fast trafficking proteins such as UBC9, Fibrillarin and have successfully characterized their differential motion between subnuclear structures. We describe here step‐by‐step procedures, with emphasis on cellular imaging parameters, to successfully perform the dynamic imaging and photoconversion of Dendra2‐fused proteins at high spatial and temporal resolutions necessary to characterize the trafficking pathways of proteins.