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1.
A 73-year-old HTLV-1 infected male developed overt ATL following 3-years observation, and the clonality of HTLV-1 infected cells changed before overt ATL onset. 10 micrograms of DNA, extracted from mononuclear cells was digested with PstI, and the clonal proliferation of HTLV-1 infected cells was examined by Southern blotting with LTR probe. 4 bands were observed 3 years prior to ATL onset, and 2 different bands were detected 2 years and 6 months later. This suggests that in some ATL cases, the clonality of monoclonally proliferated HTLV-1 infected cells may be changeable before overt ATL onset. 相似文献
2.
Hamada H. Hirano K. Goda N. Abe H. Hasegawa I. Okita Y. Arioka S. Niina T. 《Electronics letters》1994,30(20):1719-1720
The off state current for polysilicon thin-film transistors for pixels in LCDs has been successfully reduced by introducing a polysilicon buffer layer between a polysilicon active layer fabricated by solid-phase-crystallisation (SPC) and a fused quartz substrate. Off-slate current of less than 1 pA under Vgs=-25 V at Vds=3 V was obtained for n-channel single-gate coplanar transistors using the buffer layer (400 Å) 相似文献
3.
We study the problem of segmenting a sequence into k pieces so that the resulting segmentation satisfies monotonicity or unimodality constraints. Unimodal functions can be used
to model phenomena in which a measured variable first increases to a certain level and then decreases. We combine a well-known
unimodal regression algorithm with a simple dynamic-programming approach to obtain an optimal quadratic-time algorithm for
the problem of unimodal k-segmentation. In addition, we describe a more efficient greedy-merging heuristic that is experimentally shown to give solutions
very close to the optimal. As a concrete application of our algorithms, we describe methods for testing if a sequence behaves
unimodally or not. The methods include segmentation error comparisons, permutation testing, and a BIC-based scoring scheme.
Our experimental evaluation shows that our algorithms and the proposed unimodality tests give very intuitive results, for
both real-valued and binary data.
Niina Haiminen received the M.Sc. degree from the University of Helsinki in 2004. She is currently a Graduate Student at the Department
of Computer Science of University of Helsinki, and a Researcher at the Basic Research Unit of Helsinki Institute for Information
Technology. Her research interests include algorithms, bioinformatics, and data mining.
Aristides Gionis received the Ph.D. degree from Stanford University in 2003, and he is currently a Senior Researcher at the Basic Research
Unit of Helsinki Institute for Information Technology. His research experience includes summer internship positions at Bell
Labs, AT&T Labs, and Microsoft Research. His research areas are data mining, algorithms, and databases.
Kari Laasonen received the M.Sc. degree in Theoretical Physics in 1995 from the University of Helsinki. He is currently a Graduate Student
in Computer Science at the University of Helsinki and a Researcher at the Basic Research Unit of Helsinki Institute for Information
Technology. His research is focused on algorithms and data analysis methods for pervasive computing. 相似文献
4.
Occurrence of pharmaceuticals in river water and their elimination in a pilot-scale drinking water treatment plant 总被引:10,自引:0,他引:10
The occurrence of four beta blockers, one antiepileptic drug, one lipid regulator, four anti-inflammatories, and three fluoroquinolones was studied in a river receiving sewage effluents. All compounds but two of the fluoroquinolones were observed in the water above their limit of quantification concentrations. The highest concentrations (up to 107 ng L(-1)) of the compounds were measured during the winter months. The river water was passed to a pilot-scale drinking water treatment plant, and the elimination of the pharmaceuticals was followed during the treatment. The processes applied by the plant consisted of ferric salt coagulation, rapid sand filtration, ozonation, two-stage granular activated carbon filtration (GAC), and UV disinfection. Following the coagulation, sedimentation, and rapid sand filtration, the studied pharmaceuticals were found to be eliminated only by an average of 13%. An efficient elimination was found to take place during ozonation at an ozone dose of about 1 mg L(-1) (i.e., 0.2-0.4 mg of O3/ mg of TOC). Following this treatment, the concentrations of the pharmaceuticals dropped to below the quantification limits with the exception of ciprofloxacin. Atenolol, sotalol, and ciprofloxacin, the most hydrophilic of the studied pharmaceuticals, were not fully eliminated during the GAC filtrations. All in all, the treatment train was found to very effectively eliminate the pharmaceuticals from the rawwater. The only compound that was found to pass almost unaffected through all the treatment steps was ciprofloxacin. 相似文献
5.
Development and validation of a near-infrared method for the quantitation of caffeine in intact single tablets 总被引:2,自引:0,他引:2
A near-infrared spectroscopic method was developed and validated for determining the caffeine concentration of single and intact tablets in a Finnish pharmaceutical product containing 58.82% (m/m) caffeine.The spectral region of interest contained a total of 474 data points. The second derivative of Savitsky-Golay, a standard normal variate, and mean centering were used as spectral preprocessing options. The feasibility study showed nonuniformity of caffeine repartition within each tablet. Thus, spectra were recorded from both faces of the tablets, and the analysis result for a single tablet was reported as the average of both face determinations. Precision of the method was validated because the relative standard deviations from repeatability and intermediate precision tests were below 0.75% (m/m). Accuracy validation proved that the NIR results were not significantly different (P = 0.09, n = 12) from the results obtained with the reference HPLC method. The limit of quantification for caffeine was 13.7% (m/m) in the tablets. The method was found to be unaffected by NIR source replacement, but the repeatability of the results was affected if the sample holder was not placed in the correct position in the light beam. Routine NIR analysis of caffeine in tablet form was found to be more flexible and much faster than that performed with the HPLC method. 相似文献
6.
Heikki Kallio Saska Tuomasjukka Anu Johansson Raija Tahvonen Niina Nieminen Olli Sjvall Juha‐Pekka Kurvinen Hanna Kivini 《European Journal of Lipid Science and Technology》2005,107(2):101-106
Currant oils have special health properties due to their moderate contents of α‐linolenic, γ‐linolenic and stearidonic acids. The distribution of fatty acids (FA) in the triacylglycerols (TAG) may affect the beneficial effects. Seed oils of wild northern red currant (NRC) (Ribes spicatum L.) from Northern Finland and of wild alpine currant (AC) (R. alpinum L.) from the South‐West coast of Finland were investigated. The purified TAG were analysed by tandem mass spectrometry by applying the ammonia negative ion chemical ionisation – collision‐induced dissociation method. Molecular weight fractions rich in C18:3 FA and C18:4 FA were investigated. Of the total oil, the molecular weight species 54:7 (ACN:DB), 54:8 and 54:9 were more abundant in NRC than in AC, being 21.0%, 15.8%, 7.4% and 16.2%, 11.2%, 4.8%, respectively (p <0.05). The species 52:6 was more abundant in AC (3.1%) than in NRC (2.6%) (p <0.05). The preferential order of FA to be in the sn‐2 position in both berries was typically C18:1 > C18:2 > C18:4 > C18:3. No difference was observed between relative locations of C16:0 FA and C18:3 FA in either of the oils. Within the TAG consisting of FA combinations C18:3/C18:3/C18:1 (54:7), C18:1 was more preferentially in the sn‐2 position (p <0.05) in AC (93.2%) than in NRC (74.6%), and in the case of C18:3, the preference was vice versa. Within the molecular weight species 54:9, FA combination C18:4/C18:3/C18:2, linoleic acid preferentially occupied the secondary position (p <0.005) in both berries, and the proportion of the TAG regioisomer pair sn‐C18:3‐C18:4‐C18:2 + sn‐C18:2‐C18:4‐C18:3 was more abundant (30.2%) in NRC than in AC (15.3%). Within the TAG species 52:6, proportions of all the existing combinations, C16:0/C18:3/C18:3, C16:0/C18:4/C18:2 and C16:1/C18:3/C18:2, varied between the two berry species (p <0.005). 相似文献
7.
A gene (POF1) has been cloned, which confers upon yeast (Saccharomyces cerevisiae) the ability to decarboxylate phenolic acids such as ferulic and trans-cinnamic acid. This property was previously shown to be a cause of phenolic off-flavour production in wort fermentations. The identity of the cloned gene was confirmed as POF1 by gene disruption techniques. Southern blotting of total genomic DNA revealed that sequences homologous to POF1 are conserved in Pof? brewing strains of Sacch. cerevisiae. The transformation of a Pof? lager strain with the cloned POF1 gene led to the production of an aroma characteristic of a phenolic off-flavour, when the transformed strain was used in wort fermentations. This latter observation suggests that the Pof? phenotype of brewers' yeast is specifically due to the absence of a functional POF1 gene. 相似文献
8.
This study examined the effects of chemosensory performance of two age groups on ratings of the just-right intensity of odor, flavor and coolness. Two experiments were conducted: (1) creation of a sensory profile by a trained panel and (2) hedonic testing of the yogurt samples by young (n=47, 20–35 years) and elderly (n=45, 65–82 years) subjects, who also participated in tests measuring their chemosensory performance. Eight yogurt samples (2×2×2 factorial design) were flavored with 6 or 10% sucrose, 0.2 or 0.8% lemon aroma and 0.004 or 0.012% menthol. Young subjects outperformed elderly in the olfactory, gustatory and trigeminal perception tests. Their performance in the chemosensory tests affected hedonic ratings of yogurts, especially in the case of the elderly. Among the elderly, just-right flavor was affected by the performance in olfactory, gustatory (sweet, sour) and trigeminal (menthol) tests. Just-right coolness was affected by olfactory perception and by identification of sweet taste. Pleasantness was affected by olfactory and menthol perception. Thus, stimulus-specific losses in chemosensory acuity of the elderly may lead to distorted perception of foods. 相似文献
9.
The paper considers the use of mixed methods in international business research by reviewing empirical studies in four major journals. A total of 484 articles were reviewed, 68 of which had applied a mixed method strategy. In our analysis we categorize mixed methods according to their use in different stages of the research process and provide a range of alternative designs for mixing qualitative and quantitative methods. 相似文献
10.
Niina MM Saviranta Mikko J Anttonen Atte von Wright Reijo O Karjalainen 《Journal of the science of food and agriculture》2008,88(1):125-132
BACKGROUND: Red clover (Trifolium pratense L.), a widely used fodder, has recently received considerable interest as a valuable source of isoflavones for many health‐related applications. The aim of this study was to analyse the concentrations of four isoflavones, daidzein, genistein, formononetin and biochanin A, in extracts of leaves, stems, roots and different coloured flowers from red clover by high‐performance liquid chromatography and to determine the antifungal activities of the extracts. RESULTS: Among greenhouse‐grown samples the highest amounts of daidzein and genistein were found in petioles (0.11–0.28 and 0.30–0.54 mg g?1 respectively), while leaves were rich in formononetin and biochanin A (5.57–9.05 and 10.94–14.59 mg g?1 respectively). High formononetin concentrations were found in roots, but very little biochanin A. Among field‐grown samples the highest concentrations of daidzein and genistein were detected in stems collected in August (0.24 and 0.55 mg g?1 respectively), while the highest amounts of formononetin and biochanin A were found in young leaves collected in June (7.47 and 9.69 mg g?1 respectively). Most of the extracts inhibited the growth of Drechslera teres, while the root extract showed the strongest effect against Microdochium nivale. CONCLUSION: High levels of bioactive compounds found not only in inflorescences but also in roots, leaves and stems suggest that red clover may be an inexpensive and valuable resource for many applications. Copyright © 2007 Society of Chemical Industry 相似文献