Spin-lattice relaxation in ammonium compounds with a complex molecular dynamics

Expressions are derived for the initial relaxation rate 1/T1 of protons and deuterons of nontunnelling NH4 and ND4 groups reorienting about various symmetry axes in solids. The reorientation rates are modified by a trigonal, tetragonal or monoclinic distortion of the predominantly cubic hindering potential. When the rates differ sufficiently from each other, two T1 minima are observed with a characteristic ratio. Experiments were performed in NH4VO3, (NH4)2S2O8, (NH4)2PtCl4, and their deuterated modifications, which all exhibit two T1 minima. In NH4VO3 and ND4VO3 the relaxation and spectral data agree rather well with the model of trigonal distortion. Also (NH4)2S2O8 has a preferred threefold axis but there, the large tunnel splitting of protons has to be taken into account before an agreement is reached. All the purely reorientational models fail with (NH4)2PtCl4, where, instead, the ammonium groups are proposed to be ordered into domains at low temperatures. The groups inside the domains and boundary regions give rise to the high- and low-temperature T1 minima, respectively. The boundaries are also believed to give rise to the narrow component in the deuteron spectrum at low temperatures. Evidence for a proton tunnelling frequency of 32 MHz is found in (NH4)2PtCl4.     

Low power high-performance radio frequency oscillator for driving ion traps

We report a simple, efficient, high voltage radio frequency (RF) generator powered by a single voltage source (1.5-7 V) to resonantly drive ion traps or other capacitive loads. Our circuit is able to deliver RF voltages > 500?V(p-p) at frequencies ranging from 0.1 to 10 MHz. This RF oscillator uses low-cost, commercially available components, and can be easily assembled onto a circuit board of a few cm(2). Because of its simplicity and good efficiency, this circuit is useful in applications requiring small size and low power consumption such as portable ion trap systems where the duration of operation under battery power is of concern.     

The next generation models for crops and agro-ecosystems

Growth in population, decrease in arable land area, and change in climate are endangering our food security. Precision agriculture has the potential to increase crop productivity thorough tailored agricultural practices for different growing areas. Many models of crops and agro-ecosystems capable of predicting interaction between plants and environments have been developed for precision agriculture. Currently, there are several representative categories of crop and agro-ecosystem models, including the de Wi...     

重庆大剧院

<正>一场戏剧、一台音乐剧或者歌剧都是将人生现实与梦幻、想象、追求和愉悦融于一体的最高艺术形式。观看一场出色的艺术演出意味着庆贺人生,摆脱琐碎的日常生活。演出时而华美而细腻,时而崇高而妩媚,时而冷峻而现实,时而又滑稽而富有趣味,但本质上,它始终是一个非常特殊的社会生活事件。     

Suppression of tumorigenicity of rat liver epithelial tumor cell lines by a putative human 11p11.2-p12 liver tumor suppressor locus

We have previously identified and mapped a locus within human chromosome 11p11.2-p12 that suppresses the tumorigenic potential of a rat liver tumor cell line (termed GN6TF) which contains well defined chromosomal aberrations involving rat chromosomes 1, 4, 7, and 10. In the present study, we investigated the potential of this human 11p11.2-p12 liver tumor suppressor locus to suppress the tumorigenic potential of two other rat liver tumor cell lines (GN3TG and GP10TA) following microcell-mediated introduction of human chromosome 11. These tumor cell lines are aneuploid and contain chromosomal abnormalities that are similar to the GN6TF tumor line. The tumorigenic potential and other phenotypic characteristics of GN3TG-11neo and GP10TA-11neo microcell hybrid (MCH) cell lines were variable, and dependent upon the status of the introduced human chromosome 11. MCH cell lines that retained the region of 11p11. 2-p12 delineated by microsatellite markers D11S1385 and D11S903 exhibited suppression of tumorigenicity in vivo (decrease in tumorigenicity and/or elongation of latency), whereas, the tumorigenic potential of one MCH line that lacked markers in this region of human 11p11.2-p12, but retained flanking markers, was not changed from that of the parental tumor cell line. The chromosomal interval between microsatellite markers D11S1385 and D11S903 encompasses the previously localized minimal liver tumor suppressor region, suggesting that a common locus is responsible for tumor suppression among the rat liver tumor cell lines examined. The results of the present study have verified the presence of a liver tumor suppressor locus within human 11p11.2-p12, and have identified a substantial number of microsatellite markers that are closely linked to this tumor suppressor region. These chromosomal markers will facilitate positional cloning of candidate genes from this region, and may prove useful for determining the involvement of this locus in the pathogenesis of human liver cancer.     

Protein 4.1R-deficient mice are viable but have erythroid membrane skeleton abnormalities

A diverse family of protein 4.1R isoforms is encoded by a complex gene on human chromosome 1. Although the prototypical 80-kDa 4.1R in mature erythrocytes is a key component of the erythroid membrane skeleton that regulates erythrocyte morphology and mechanical stability, little is known about 4.1R function in nucleated cells. Using gene knockout technology, we have generated mice with complete deficiency of all 4.1R protein isoforms. These 4.1R-null mice were viable, with moderate hemolytic anemia but no gross abnormalities. Erythrocytes from these mice exhibited abnormal morphology, lowered membrane stability, and reduced expression of other skeletal proteins including spectrin and ankyrin, suggesting that loss of 4. 1R compromises membrane skeleton assembly in erythroid progenitors. Platelet morphology and function were essentially normal, indicating that 4.1R deficiency may have less impact on other hematopoietic lineages. Nonerythroid 4.1R expression patterns, viewed using histochemical staining for lacZ reporter activity incorporated into the targeted gene, revealed focal expression in specific neurons in the brain and in select cells of other major organs, challenging the view that 4.1R expression is widespread among nonerythroid cells. The 4.1R knockout mice represent a valuable animal model for exploring 4.1R function in nonerythroid cells and for determining pathophysiological sequelae to 4.1R deficiency.     

Millenium Biologix总部

项目地点:加拿大安大略省京士顿项目类型:生物技术公司总部开工日期:未知该项目获2005年豪瑞可持续建筑大奖赛北美地区提名奖     

布鲁塞尔弗拉基文场

圣克洛克斯广场、弗拉基广场和湖区是一个充满活力的多样化城市公共空间.弗拉基广场作为其中历史悠久的中心区域,具有至关重要的地位.因此设计需要将弗拉基广场与整个城市氛围相融合,并且为行人创造一处连续的人性场所.     

仪式的进程 瑞士塔米纳温泉浴场

位于瑞士巴特拉加茨(Bad Ragaz)的塔米纳(Tamina)温泉浴场是整个格兰德度假村(Grand Resort)的一个重要组成部分。巴特拉加茨地区是瑞士古往今来著名的温泉胜地,每年都吸引着众多游人前来     

Epstein-Barr-based episomal chromosomes shuttle 100 kb of self-replicating circular human DNA in mouse cells

We describe the microcell fusion transfer of 100-200 kb self-replicating circular human minichromosomes from human into mouse cells. This experimental approach is illustrated through the shutting of the latent 170 kb double-stranded DNA genome from the human herpesvirus, Epstein-Barr virus, into nonpermissive rodent cells. Using this interspecies transfer strategy, circular episomes carrying 95-105 kb of human DNA were successfully established at low copy number in mouse A9 cells. Selected episomes were stably maintained for 6 months, and unselected episomes were characterized by a 95% episomal retention per cell division. The establishment of a mouse artificial episomal chromosome system should facilitate evolutionary and therapeutic studies of large human DNA in rodent genetic backgrounds.