The distribution of HBV, HCV and HGV among livers with fulminant hepatic failure of different aetiology

BACKGROUND/AIMS: The aim of the study was to assess the impact factor of HCV and HGV in fulminant hepatic failure. METHODS: The 5'-untranslated regions of HCV RNA and HGV RNA and a segment of the core antigen sequence of HBV were amplified after extracting the nucleic acids from snap-frozen tissue aliquots from explanted livers of 26 consecutive patients undergoing orthotopic liver transplantation for fulminant hepatic failure preoperatively diagnosed as either autoimmune (n=2), HAV/HBV (n=8), toxic (n=4) or aetiologically unknown (n=12). RESULTS: HCV RNA was detected in five of 26 (19.2%) livers with fulminant hepatic failure. All five HCV RNA-positive livers belonged to the group of non-toxic, non-autoimmune liver failure (n=20), three of them were found in the group of liver failure with unknown aetiology (n=12) and two in the group of HBV-associated liver failure (n=7), making an HCV incidence of 25%, 25% and 28.6%, in the different groups, respectively. HGV RNA was detected in 10 of 17 (58.8%) explants and in all four groups of fulminant hepatic failure as defined preoperatively. HBV DNA was identified in six livers of 26 patients (23.1%) with fulminant hepatic failure. Neither HCV RNA nor HBV DNA was detected in the livers of patients with toxic or autoimmune fulminant hepatic failure. CONCLUSIONS: These results indicate that HBV and HCV, but not HGV, play an aetiologic role in fulminant hepatic failure. HCV-positive cases were concentrated either in the group of otherwise unexplained fulminant hepatic failure or in the group of HBV fulminant hepatic failure. HGV-positive cases, on the other hand, were found within all four preoperatively defined groups, indicating a role as cofactor rather than as single aetiologic agent.     

Is implementation of continuous improvement possible? An evolutionary model of enablers and inhibitors

The purpose of this article is to present an evolutionary model of the enablers and inhibitors affecting the implementation and sustainability of continuous improvement. With this aim, a qualitative study using the grounded theory was carried out. Interviews were conducted with managers responsible for continuous improvement in first‐tier suppliers in the automotive sector. As a result, two existing lines of research in the continuous improvement literature are synthesized: on one hand, the literature concerning the enablers and barriers associated with continuous improvement sustainability; and on the other, the literature concerning the evolutionary model. © 2011 Wiley Periodicals, Inc.     

Genetic Contribution of Endometriosis to the Risk of Developing Hormone-Related Cancers

Endometriosis is a common gynecological disorder that has been associated with endometrial, breast and epithelial ovarian cancers in epidemiological studies. Since complex diseases are a result of multiple environmental and genetic factors, we hypothesized that the biological mechanism underlying their comorbidity might be explained, at least in part, by shared genetics. To assess their potential genetic relationship, we performed a two-sample mendelian randomization (2SMR) analysis on results from public genome-wide association studies (GWAS). This analysis confirmed previously reported genetic pleiotropy between endometriosis and endometrial cancer. We present robust evidence supporting a causal genetic association between endometriosis and ovarian cancer, particularly with the clear cell and endometrioid subtypes. Our study also identified genetic variants that could explain those associations, opening the door to further functional experiments. Overall, this work demonstrates the value of genomic analyses to support epidemiological data, and to identify targets of relevance in multiple disorders.     

Biofilm Responses to Flow Regulation by Dams in Mediterranean Rivers

Dams regulate downstream hydrology and modify water quality, which in turn can impinge on the biota, especially in rivers naturally subject to large hydrological variability, such as those under Mediterranean climate. The effect of dams on biofilms was analysed in three tributaries (Cinca, Siurana and Montsant) of the Ebro River (NE Spain). We hypothesized that flow regulation would lead to lower spatial variability of biofilms on the streambed and to a decrease in their metabolic rate per unit biomass, especially during low flow periods. Biofilm characteristics were studied in five transects evenly spaced along river reaches upstream (control) and downstream (impact) of dams in each river, along with riverbed granulometry, hydraulics and water chemistry. Chlorophyll‐a, respiratory activity, photosynthetic capacity and efficiency, and extracellular enzymatic activities (β‐d ‐glucosidase, alkaline phosphatase and leucine‐amino‐peptidase) of epilithic biofilms were measured in different seasons. Spatial variability of chemical and biological variables was reduced downstream of the dams. Chlorophyll‐a concentration, photosynthetic efficiency and respiration capacity were higher in impact than in control reaches, but generally, low inorganic phosphorus concentrations resulted in comparable phosphatase activities downstream and upstream of dams. On the other hand, β‐d ‐glucosidase and leucine‐amino‐peptidase activities were higher at impact reaches. Biofilms were thicker and metabolically more active at the impact reaches, with higher ability to transform dissolved organic matter. Overall, results from this study provide evidence that dams can largely affect the structure and activity of river biofilms, with foreseeable important consequences for river ecosystem functioning. Copyright © 2014 John Wiley & Sons, Ltd.     

Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell

Dual-color fluorescence correlation spectroscopy is an interesting method to quantify protein interaction in living cells. But, when performing these experiments, one must compensate for a known spectral bleed through artifact that corrupts cross-correlation data. In this article, problems with crosstalk were overcome with an approach based on fluorescence lifetime correlation spectroscopy (FLCS). We show that FLCS applied to dual-color EGFP and mCherry cross-correlation allows the determination of protein-protein interactions in living cells without the need of spectral bleed through calibration. The methodology was validated by using EGFP-mCherry tandem in comparison with coexpressed EGFP and mCherry in live cell. The dual-color FLCS experimental procedure where the different laser intensities do not have to be controlled during experiment is really very helpful to study quantitatively protein interactions in live sample.     

Internet in teachers' professional practice outside the classroom: Examining supportive and management uses in primary and secondary schools

In recent years there has been widespread interest in the implementation of information and communication technologies (ICT) in schools. While most studies primarily focus on the use of ICT in teaching and learning, little attention has been given to their incorporation as a professional tool outside the classroom. Using a digital inequality approach, the aim of this paper is to develop and test a model of the factors that affect teachers' professional use of ICT, particularly the Internet, in primary and secondary schools. One thousand four hundred and five (1405) teachers from a representative sample of 536 primary and 273 secondary schools in Spain were surveyed. The dependent variables include attitudes towards the professional use of ICT and the use of the Internet in professional practice. The explanatory variables are the socio-demographics, school-level information, frequency of Internet access within and outside the school, educational ICT training, digital literacy, and organisational development. Controlling for socio-demographics and school-level information, multiple regression analyses are used to make inferential judgements and test the separate effects of the independent variables. Findings suggest that technological factors and organisational practices are important predictors of ICT appropriation for professional purposes.     

Double actuation architectures for rendering variable impedance in compliant robots: A review

Novel compliant actuation systems have been developed in recent years for a variety of possible advantages, such as establishing a safe human–robot interaction, increasing energy efficiency, reducing the effects of impacts and even for the development of neuro-inspired robotic platforms to be used in human motor control studies.In this rapidly growing and transversal research field, systems involving more than one active element (typically motors) for each actuated degree of freedom are being investigated to allow separate position and impedance regulations. Considering the wide range of applications and the large number of different arrangements deriving from the combination of two active elements and passive elastic components, several actuation architectures have been devised.This paper reviews state-of-the-art rotary variable impedance units incorporating two separate motors. Existing devices are grouped in three main categories. A critical and comparative analysis of the most relevant features is carried out, also based on most representative prototypes. Recently proposed methodologies and evaluation criteria for design optimization are illustrated and perspectives on potential applications of double actuation systems are presented.     

Chasing real-time interaction in new media: towards a new theoretical approach and definition

‘Real-Time’ is one of the most frequent concepts used referring to digital media. Despite it is commonly related to immediate feedback, it is not clear when it happens and what its characteristics are, especially in current digital interfaces. Although real-time has been written about very extensively in the context of Human Computer Interaction and Computational Systems, there are few studies evaluating the key features of real-time interaction in other domains. This article attempts to address this situation by proposing a new approach to the concept of real-time interaction driving the analysis of this topic into paradigmatic cases studies in new media, video games and digital artworks. This new analysis reveals several specific facets that allow us to identify new factors in the understanding of this fuzzy concept. Finally, the article proposes a new definition of ‘real-time interaction’ based on the findings in the context of new media practices.