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THE EFFECT OF AEROBIC MESOPHILIC MICROFLORAL LEVELS ON THE ISOLATION OF INOCULATED LISTERIA MONOCYTOGENES STRAIN LM82 FROM SELECTED FOODS 总被引:1,自引:0,他引:1
The effect of aerobic mesophilic microfloral concentration on the isolation of Listeria monocytogenes LM82 was studied in 31 (18 cheeses and 7 noncheese) retail foods having standard plate counts of 101 to 108 colony forming units (CFU)/g. Foods were spiked with L. monocytogenes and enriched at 30°C for 24 h in a selective enrichment broth used in a U.S. Food and Drug Administration method. Inoculum levels for isolation on modified McBride agar ranged from 0.1 to > 5 × 103 with a geometric mean value of 5 inoculated CFU/g or 1.4 CFU/g. Pure Enterococcus (Streptococcus) faecalis ( 0 to 6 × 106 inoculated CFU/mL ) in the absence of food matrix had no effect on the enrichment of L. monocytogenes. Ease of isolation of LM82 was independent of the food microflora concentration both generally and in the specific food type of 9 Brie cheeses. Competition, when it occurs, therefore, may be due to specific bacterial competitors rather than bacterial numbers . 相似文献
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TONY GRIFT 《Particulate Science and Technology》2013,31(2):177-193
A generic mass flow measurement device was developed as a variation on the theme of counting. In a hypothetical infinitely sparse mass flow, the number of passing particles could be counted in a time frame and multiplied by the mean mass per particle to obtain a mass flow per time unit. In a mass flow of realistic density, however, particles travel in cluster formation and direct counting of individual particles is impossible. If a method could be available that reconstructs the original number of particles in a cluster, the mass flow can be computed for realistic clustered mass flows. This reconstruction algorithm was developed in this research; it uses the measured cluster lengths to reconstruct the total number of particles in each passing cluster. The lengths of the clusters were measured with an optoelectronic device. The reconstruction algorithm was developed using simulation, augmented by clustering theory. For identical diameter particle flow, simulation results showed that the number of particles in a cluster could be reconstructed using a very simple reconstruction formula. This formula uses only the total number of clusters per time frame and the total number of individual particles measured in the same time frame. However, identical diameter flow is not realistic, since even identical particles are measured with a certain error. Reconstruction of the realistic distributed diameter particle flow was approximated using the identical particle method. The optical mass flow sensor has major advantages over traditional methods. It is virtually insensitive to vibrations, contamination, temperature drift, and misalignment and the underlying measurement concept is well understood. But most importantly, the sensor does not require calibration. The mass flow of identical particles (4.5 mm air gun pellets) was measured with an error smaller than 3% even for high density flow rates. 相似文献
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A constructionist approach to the study of social problems and housing policy provides a theoretically informed means of analysing the ways in which housing policy is formulated and implemented. Yet despite a strong commitment by housing researchers to policy relevance, constructionist studies of how specific social problems are generated and deployed have so far made only a limited impact on housing research. The paper addresses this lacuna by first discussing important literature and the key conceptual issues in this field of study. This is followed by a discussion of two examples from recent UK housing policy (the shift in the 1980s from defining lone mothers as the victims of housing shortages to a morally questionable group subverting needs-based allocation policies and the re-emergence of anti-social behaviour as a problem on housing estates). The paper's conclusion is that the 'construction of problems' provides a rich source of new material as well as offering significant opportunities to develop a more critically informed housing research agenda. 相似文献
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Various amounts of nisin (0, 103 and 5 × 103 IU/g) in combination with either potassium sorbate (0, 2, and 3%) or sodium benzoate (0, 0.06 and 0.12%) were tested for effectiveness in inhibiting growth of Staphylococcus aureus C10 and Bacillus cereus B7 inoculated on a vegetarian food. The strains used were isolated from vegetarian foods obtained commercially in Taiwan, and the test food, spice and dried bean curd, was selected for the study based on ability to support the growth of these organisms. After treatment with a preservative combination, the surfaces of sterilized food samples were inoculated, samples were stored in vacuum or nonvacuum packages at either 4C or 30C, and at appropriate times, tested for microbial growth. Growth of both isolates was unaffected by vacuum-packaging treatment; however, a bacteriostatic effect was found at 4C. Data indicated that during the 14-day storage at 4C, vacuum-packaged samples treated with 5 × 103 IU/g nisin and 0.12% sodium benzoate significantly (p < 0.05) decreased the counts of S. aureus C10 and B. cereus B7 by 2.61 and 3.02 log10 CFU/g, respectively. In the vacuum-packaged samples treated with 5 × 103 IU/g nisin and 3% potassium sorbate, counts for C10 and B7 were decreased by 2.35 and 2.64 log10 CFU/g, respectively. Thus, the combined treatment extended the shelf-life of the vegetarian food . 相似文献
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TONY K. WOLF PETER R. DRY PATRICK G. ILAND DAVID BOTTING JOY DICK URSULA KENNEDY RENATA RISTIC 《Australian Journal of Grape and Wine Research》2003,9(2):82-95
Own‐rooted Shiraz, spaced 1.5 m apart in 2.75 m wide rows, were compared under five different training systems in the Barossa Valley, South Australia over five seasons starting 1995–96. Our objective was to determine how training affected fruit composition and crop yield in a context of efficient vineyard management. Training systems were: (i) low single wire (LSW), in which vines were trained to bi‐lateral cordons, 1.0 m above the ground, non‐shoot‐positioned; (ii) high single wire (HSW), a higher cordon (1.8 m) version of LSW; (iii) vertically shoot positioned (VSP); (iv) Scott Henry, where canopies of alternate vines were cordon‐trained and shoot‐positioned upwards (SHU) or downwards (SHD); and (v) minimally pruned (MIN), the same as LSW, except vines were not annually spur‐pruned. Crop yields (kg/m of row), over four seasons, averaged 4.9 (MIN), 2.9 (combined Scott Henry), 3.2 (VSP), 2.6 (HSW) and 2.9 (LSW). The greatest year‐to‐year variation in yield occurred with MIN and Scott Henry training. Crop weight of SHD vines was inferior to that of SHU vines in two seasons due to fewer shoots and bunches per vine, and to fewer bunches per shoot. Individual berry weights (g) were consistently least with MIN (0.89), and greatest with VSP (1.17). Fruit from all training systems exhibited similar rates of sugar accumulation during two seasons in which repeated measures of fruit maturation were made. Excepting the relatively light yields observed in 1999–2000, sugar accumulation was delayed in MIN vines, relative to other training systems, even when MIN harvest was delayed up to two weeks. The delay is most readily explained by the greater crops of MIN vines. Berry total anthocyanins and total phenolics concentrations (mg/g berry fresh wt.) at harvest were not greatly affected by training system. Berry anthocyanins and total phenolics exhibited a negative relationship with crop/m of canopy and a slight positive relationship with bunch exposure when evaluated across all training systems. Experimental plot soil depth and water availability affected cane pruning weights, yield per vine, berry weight, and canopy characteristics. LSW, HSW and MIN training systems all provided good yields of high quality fruit, although MIN did have a tendency to produce excessive crops in some years. VSP and Scott Henry training were less attractive due to their inherently greater canopy management requirements. 相似文献
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INVESTIGATED THE DEGREE TO WHICH STUDENT CLINICIANS DIFFERENTIATE AMONG CLIENTS AND SIGNIFICANT OTHERS AS ROLE TYPES, AND TO WHICH THE DEGREE OF DIFFERENTIATION VARIES AS A FUNCTION OF THE AFFECTIVE VALUE OF THE STIMULUS PERSON. COGNITIVE COMPLEXITY, DERIVED FROM A MODIFICATION OF KELLY'S ROLE CONSTRUCT REPERTORY TEST, WAS USED AS AN INDEX OF DIFFERENTIATION. RESULTS INDICATE THAT MORE DIFFERENTIATIONS WERE MADE AMONG CLIENTS THAN SIGNIFICANT OTHERS. THE DEGREE OF DIFFERENTIATION MADE AMONG CLIENTS WAS NOT AFFECTED BY THE AFFECTIVE VALUE OF THE STIMULUS PERSON, WHEREAS, WITH SIGNIFICANT OTHERS, SS MADE SIGNIFICANTLY MORE DIFFERENTIATIONS AMONG THOSE ASSOCIATED WITH NEGATIVE AFFECT. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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ABSTRACT While design procedures for conventional induction and synchronous machines are now mature and constrained by frame sizes and standards, there are opportunities to use modem CAD techniques to exploit the unique capabilities and characteristics of permanent magnet synchronous motors (PMSM). This paper presents a design methodology involving the use of an analytical model based on generalized machine theory, complemented by finite element analysis. The synergistic interaction of these two methods allows an engineer to design a PMSM to specific requirements with confidence. The effects of machine airgap and of the span angle and thickness of the permanent magnets are evaluated. A set of design specifications and a goodness function are established to select the preferred rotor geometry for a 30 hp prototype permanent magnet synchronous motor. 相似文献