Characterization of activated sludge flocs by confocal laser scanning microscopy and image analysis

In this study we present a new approach to determine volumes, heterogeneity factors, and compositions of the bacterial population of activated sludge flocs by 3D confocal imaging. After staining the fresh flocs with fluorescein-isothiocyanate, 75 stacks of images (containing approx. 3000 flocs) were acquired with a confocal laser scanning microscope. The self-developed macro 3D volume and surface determination for the KS 400 software package combined the images of one stack to a 3D image and calculated the real floc volume and surface. We determined heterogeneity factors like the ratio of real floc surface to the surface of a sphere with the respective volume and the fractal dimension (D(f)). According to their significant influence on floc integrity and quality, we also investigated the chemical composition of flocs and quantified their bacterial population structure by using group-specific rRNA-targeted probes for fluorescence in situ hybridization. By a settling experiment we enriched flocs with poor settling properties and determined the above-mentioned parameters. This approach revealed shifts in floc volume, heterogeneity, and bacterial and chemical composition according to the settling quality of the flocs.     

Cytotoxicity of CeO2 nanoparticles for Escherichia coli. Physico-chemical insight of the cytotoxicity mechanism

The production of nanoparticles (NPs) is increasing rapidly for applications in electronics, chemistry, and biology. This interest is due to the very small size of NPs which provides them with many interesting properties such as rapid diffusion, high specific surface areas, reactivity in liquid or gas phase, and a size close to biomacromolecules. In turn, these extreme abilities might be a problem when considering a potentially uncontrolled exposure to the environment. For instance, nanoparticles might be highly mobile and rapidly transported in the environment or inside the body through a water or air pathway. Accordingly, the very fast development of these new synthetic nanomaterials raises questions about their impact on the environment and human health. We have studied the impact of a model water dispersion of nanoparticles (7 nm CeO2 oxide) on a Gram-negative bacteria (Escherichia coli). The nanoparticles are positively charged at neutral pH and thus display a strong electrostatic attraction toward bacterial outer membranes. The counting of colony forming units (CFU) after direct contact with CeO2 NPs allows for the defining of the conditions for which the contact is lethal to Escherichia coli. Furthermore, a set of experiments including sorption isotherms, TEM microscopy, and X-ray absorption spectroscopy (XAS) at cerium L3 edge is linked to propose a scenario for the observed toxic contact.     

Introduction

Networks and Spatial Economics -     

Spatial interaction modelling

Spatial interaction (SI) is the process whereby entities at different points in physical space make contacts, demand/supply decisions or locational choices. The entities can be individuals or firms and the choices can include housing, jobs, production quantities, exports, imports, face-to-face contacts, schools, retail centres and activity centres. The first SI models can be grouped under the generic heading gravity models. Their main characteristic is that they model the behaviour of demand or supply segments, rather than that of individuals and firms. This article traces the development of these models from their inception in the early part of the twentieth century to the present. The key advances include the replacement of the gravity analogy by the more general concepts of entropy or information theory, a statistical framework commonly used in physics. With the arrival of the regional science paradigm over 50 years ago, a key challenge has been to broaden these models compared to those arising in spatial economics, thus arriving at a more inclusive probabilistic framework. These efforts are discussed here, as well as inclusion of geographical advances, embracing activities as generators of travel, time-geography, recognition of spatial interdependencies, and use of neuro-computing principles.JEL Classification: C190, C450, C610, R00     

The relevance of association networks for/in a sustainable information and communication society

This contribution deals with taking up the challenge of sustainable development through human centred systems which aim at the creation and repatriation of global quality in each society, and which are seen to operate as a whole, on a local, regional or even a planetary scale. The paper argues that, particularly in a field such as information, communication, environment, technological processes and innovations, which have structurally revolutionised first of all manufacturing but also education and daily living at the same time. However, producing new pathogenous structures require, by necessity, a political ecology in order to relate these fields to new figures of meditation/mediance and crossbreeding built up by associative networks. PRELUDE'S experience, as an international networking programme of scientists pursuing with other social factors objectives of codevelopments (in response to the failure of bad development in the North and in the South), and its contribution to the theme Global Perspective 2010 of the CE-FAST Programme suggest the actual relevance and, turning to the future, the decisive function of associative networks as a way of approaching more efficiently, because of their flexibility, complex, highly heterogeneous situations to be tackled in a systemic and global fashion. In complementing institutions and established companies these networks give their new performances and efficacity to institute, and so doing displace acquired balances, and increase their capacity to innovate.     

The use of Mersilene mesh in adult inguinal and femoral hernia repairs: a comparison with classic techniques

A retrospective review of both mesh classic inguinal hernia repairs performed under the guidance of a single surgeon showed that Mersilene mesh is safe to use and that the recurrence rate is significantly improved by using the mesh for repair. Mersilene mesh is easier to use than other types of mesh and should be used routinely in the repair of inguinal and femoral hernias.     

Allelopathic potential of wild oat (Avena fatua) on spring wheat (Triticum aestivum) growth

Wild oat plants may produce toxic substances that suppress the growth and development of desirable species, thus accounting for severe yield loss in infested fields. The purpose of this study was to determine the allelopathic potential of wild oat (Avena fatua) on the growth of spring wheat (Triticum aestivum var. Fieldwin) in the absence of plant competition. Wild oat and spring wheat plants were grown separately in 250-ml beakers in a sand medium. Root exudates were extracted from wild oat medium at the 1-, 2-, 3-, and 4-leaf stages of wild oat development and added to beakers containing spring wheat in temporally corresponding stages of development. Spring wheat root and leaf dry weights were measured to determine if one or more allelochemical agents were released from wild oat roots. Spring wheat leaf and root dry weights were significantly reduced by exudates from wild oat plants at the 2- and 4-leaf stages of development, respectively. Allelochemicals were isolated from wild oat root exudates at various stages of plant development. Paper chromatography analysis indicated that at least two unknown compounds were present.R _f values in benzene-acetic acid-water of the two unknown compounds (0.825 and 0.930) were similar to scopoletin (7-hydroxy-6-methoxycoumarin) and vanillic acid (4-hydroxy-3-methoxybenzoic acid), respectively. Additional tests using diazotizedp-nitraniline, ultraviolet absorption spectra, and gas chromatography analysis also indicated that the unknowns were coumarin-related compounds such as scopoletin and vanillic acid.     

Structural comparison of the Pichia pastoris alcohol oxidase genes

In methylotrophic yeasts, alcohol oxidase is the first enzyme in the methanol-utilization pathway. The genome of one such yeast, Pichia pastoris, contains two alcohol oxidase genes, AOX1 and AOX2. Sequence analysis indicated that each gene encodes a similar protein of 663 amino acids. The protein-coding regions of the genes were 92% and 97% homologous at the nucleotide and predicted amino acid sequence levels, respectively. In contrast to homology observed within the protein-coding portions of the AOX genes, no homology was found in either the 5′ or 3′ non-coding regions. Although alcohol oxidase is found in peroxisomes of P. pastoris, the AOX amino acid sequences did not contain a peptide sequence similar to the peroxisomal transport sequence found at the C-terminus of some peroxisomally located proteins in higher eukaryotes.     

Uncovering regional characteristics from mobile phone data: A network science approach

We introduce network science methods to uncover inherent characteristics of functional regions. An aggregate spatial interaction network is constructed based on a large mobile phone data set including 431 million mobile calls made by 10 million anonymous customers over one month and the geographic locations of the mobile base towers involved in each call. We use Thiessen polygons (termed ‘cells’) as the unit of analysis to approximate the service area of each mobile base tower. Major findings encompass the following three aspects. First, cells with high betweenness centrality are linearly distributed in space, which closely aligns with major transportation corridors. We find that this pattern can be explained by analysing the characteristics of calling activities on transportation networks. Second, we detect a two‐level hierarchy of communities that correspond well to county and prefecture‐level administrative unit boundaries. Lastly, almost every community identified at the two hierarchical levels contains a cell with high betweenness. These cells are located near the political and economic centres and play the role of hubs in the regional socio‐economic system. This research demonstrates that networks built from mobile phone data provide new understandings of spatial interactions and regional structures.