Towards a Better Prediction of Cell Settling on Nanostructure Arrays—Simple Means to Complicated Ends

Vertical arrays of nanostructures (NSs) are emerging as promising platforms for probing and manipulating live mammalian cells. The broad range of applications requires different types of interfaces, but cell settling on NS arrays is not yet fully controlled and understood. Cells are both seen to deform completely into NS arrays and to stay suspended like tiny fakirs, which have hitherto been explained with differences in NS spacing or density. Here, a better understanding of this phenomenon is provided by using a model that takes into account the extreme membrane deformation needed for a cell to settle into a NS array. It is shown that, in addition to the NS density, cell settling depends strongly on the dimensions of the single NS, and that the settling can be predicted for a given NS array geometry. The predictive power of the model is confirmed by experiments and good agreement with cases from the literature. Furthermore, the influence of cell‐related parameters is evaluated theoretically and a generic method of tuning cell settling through surface coating is demonstrated experimentally. These findings allow a more rational design of NS arrays for the numerous exciting biological applications where the mode of cell settling is crucial.     

The effect of appraisers in estimating metabolic rate with the Edholm scale

A study was made of the appraisers' effect on the estimation of metabolic rate with the Edholm scale and a table of the ISO 7243 heat stress standard. The appraisers, five experienced and five inexperienced persons, estimated the metabolic rate of three different work tasks from videotapes. Analysis of variance indicated significant ( [Formula: see text] ) differences in the appraisers' recordings of the activities. The appraisers were grouped according to the similarity of the estimated values they gave. The groups thus contained both experienced and inexperienced appraisers, and it was not possible to classify the appraisers into experienced and inexperienced groups according to their earlier experience. The metabolic rates according to the Edholm scale were higher than according to the ISO 7243 table. The differences in metabolic rates given by the individual observers varied from 38 to 118 W/m(2). The variations in the estimation of metabolic rates were greater when the Edholm scale was used. This variation caused considerable variation also in the predicted mean vote, PMV index. It is recommended that the appraisers be selected carefully, because it is not possible to know whether a randomly selected appraiser is an 'average' or an 'extreme' appraiser without a test. Before conducting extensive field surveys where several appraisers estimate the metabolic rates, it would be useful to arrange training in order to calibrate the levels of the Edholm scale as well as ISO method among the appraisers because training clearly unified the estimation.     

Are the desks and chairs at school appropriate?

The aim of the current study was to find out how the measures of chairs and desks match with the anthropometrics of schoolchildren and how schoolchildren sit during a lesson in their classroom. This paper reports the baseline measurements of an intervention study. Participants of this study were 6th and 8th grade (12 and 14 year old) schoolchildren from two comprehensive schools in Finland (N = 101, 57 girls and 44 boys). The main outcome measures were the differences between desk height and elbow-floor height, and chair height and popliteal height. Forty-three participants were randomized for sitting posture analysis by video recordings. The study showed that desks were on average 13 cm above elbow-floor height and chairs 2 cm below popliteal height. For 56% of time participants sat with their backs flexed >20 degrees and/or rotated >45 degrees . For 70% of time they sat with their necks flexed >20 degrees or rotated >45 degrees. The results indicate that there is a mismatch between school furniture and the anthropometrics of schoolchildren. Schoolchildren sit in disadvantaged postures for a substantial part of school lessons.     

Navigation Aided Image Processing in UAV Surveillance: Preliminary Results and Design of an Airborne Experimental System

This paper describes an airborne reconfigurable measurement system being developed at Swedish Defence Research Agency (FOI), Sensor Technology, Sweden. An image processing oriented sensor management architecture for UAV (unmanned aerial vehicles) IR/EO-surveillance is presented. Some preliminary results of navigation aided image processing in UAV applications are demonstrated, such as SLAM (simultaneous localization and mapping), structure from motion and geolocation, target tracking, and detection of moving objects. The design goal of the measurement system is to emulate a UAV-mounted sensor gimbal using a stand-alone system. The minimal configuration of the system consists of a gyro-stabilized gimbal with IR and CCD sensors and an integrated high-performance navigation system. The navigation system combines dGPS real-time kinematics (RTK) data with data from an inertial measurement unit (IMU) mounted with reference to the optical sensors. The gimbal is to be used as an experimental georeferenced sensor platform, using a choice of carriers, to produce military relevant image sequences for studies of image processing and sensor control on moving surveillance and reconnaissance platforms. Furthermore, a high resolution synthetic environment, developed for sensor simulations in the visual and infrared wavelengths, is presented. © 2004 Wiley Periodicals, Inc.     

Challenging Controversies: A Prospective Analysis of the Influence of New Technologies on the Safety of Offshore Drilling Operations

Integrated operations (IO) is an operating mode in the offshore oil and gas industry that is expected to lead to safer, faster and better operations. This article presents an analysis of the anticipated impacts of increased instrumentation on the safety of drilling operations. The instrumentation is related to the change process of IO, and is exemplified by a group of IO tools for interpretation, diagnosis and automation. An important finding in the study is the identification of a set of controversies that reflect characteristic challenges of drilling operations. These controversies involve the quantity and accessibility of information, the issue of centralized and decentralized control, the relation between standardized and unique interpretation of data, and the heterogeneous nature of engineering work. It is argued that the impact of the IO tools on safety will depend on how these controversies are taken into account when the tools are adopted. It is also argued that the cognitive control of the operations is distributed across a range of human and nonhuman actors and that the impact of the IO tools thus depends on how they are adapted to the system of distributed cognition rather than on the properties of the tools themselves.     

Numerical modeling of the hydraulic blade pitch actuator in a spar‐type floating wind turbine considering fault conditions and their effects on global dynamic responses

This paper deals with numerical modeling of the hydraulic blade pitch actuator and its effect on the dynamic responses of a floating spar‐type wind turbine under valve fault conditions. A spar‐type floating wind turbine concept is modeled and simulated using an aero‐hydro‐servo‐elastic simulation tool (Simo‐Riflex [SR]). Because the blade pitch system has the highest failure rate, a numerical model of the hydraulic blade pitch actuator with/without valve faults is developed and linked to SR to study the effects of faults on global responses of the spar‐type floating wind turbine for different faults, fault magnitudes, and environmental conditions. The consequence of valve faults in the pitch actuator is that the blade cannot be pitched to the desired angle, so there may be a delay in the response due to excessive friction and the wrong voltage, or slit lock may cause runaway blade pitch. A short circuit may cause the blade to get stuck at a particular pitch angle. These faults contribute to rotor imbalance, which result in different effects on the turbine structure and the platform motions. The proposed method for combining global and hydraulic actuator models is demonstrated in case studies with stochastic wind and wave conditions and different types of valve faults.     

Effect of surfactant concentration, pH, and shear rate on the rheological properties of aqueous systems of a hydrophobically modifed chitosan and its unmodified analogue

Oscillatory shear, shear relaxation and viscosity experiments have been carried out on semidilute solutions of chitosan and on a hydrophobically modified analogue (HM-chitosan) in 1% acetic acid. This was done in the presence of various amounts of cetyltrimethylammonium bromide (CTAB) and at different pH values. All the rheological measurements on the HM-chitosan solutions revealed significant polymer-surfactant interaction and pH effects. The observed rheological effects were least pronounced at pH ≈ 4, while a strong viscoelastic response was found at pH values of 1 and 5 in HM-chitosan solutions of low surfactant concentration. At these conditions, significant shear-thinning effects were observed. In semidilute solutions of unmodified chitosan, the influence of pH, surfactant concentration and shear rate on the rheological properties was moderate or insignificant. Received: 25 September 1996/Revised version: 30 October 1996/Accepted: 4 November 1996