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1.
Lectinocytochemistry provides a useful tool for localizing subcompartments of the complex reticular apparatus of Golgi. The technique is based on interactions of lectins with glycoconjugates present in the limiting membranes and luminal spaces of Golgi elements. Application of a series of lectins of different sugar specificities permits a differentiation between Golgi subcompartments containing glycoconjugates with different oligosaccharide side chains. These may be (a) different glycoconjugates or (b) glycoconjugates at different stages during synthesis or repair of their glycans. The lectinocytochemical studies with mannose-, glucose-, N-acetyl-glucosamine-, N-acetylgalactosamine-, galactose-, fucose-, and sialic acid-recognizing lectins revealed predominating patterns that labeled distinct, i.e., cis, medial, trans, and transmost, regions of the Golgi apparatus. A further refinement could be achieved by differential lectin-inhibition that enables a dissection of lectin binding reactions on the basis of their binding affinities. High-affinity binding reactions showed that subcompartments are not necessarily confined to one single Golgi subregion and may change their position from one to another subregion. Some of the patterns observed may be interpreted in relation to certain steps during synthesis and modifications of glycans. 相似文献
2.
Cystic fibrosis (CF) is the most common lethal autosomal recessive disorder among Caucasians and is caused by abnormalities in the cystic fibrosis transmembrane conductance regulator gene (CFTR). CFTR gene encodes a chloride channel that regulates secretion in many exocrine tissues especially pancreatic and pulmonary tissues. The clinical presentation of cystic fibrosis is highly variable with isolated CAVD (congenital absence of vas deferens) and/or typical pancreatic and pulmonary manifestations. Over 500 mutations in the CFTR gene have been described and vary among different geographic locations. The severity of clinical manifestations and specially the pulmonary disease is poorly correlated with genotype. It is interesting to collect clinical and genetical data by analysing a larger cohort of CF patients. These results are likely to improve our understanding of the physiopathology of CF and the genetic counselling; particular biochemical defect could lead to more specific treatments in the future. From our 110 patients selected in Champagne-Ardenne country, we analysed the entire coding sequence of CFTR gene and detected 95% of CF mutations and in fact, 89.5% if we include the CAVD patients; 59.4% of CF mutations were detected for these patients. Three new mutations have been here reported. We found numerous CF mutations with a large distribution throughout the gene. Nevertheless, three exons are mainly involved: 10, 11 and 21. Relationships between the genotype and phenotype are difficult to assess. 相似文献
3.
4.
Relative neighborhood graphs and their relatives 总被引:12,自引:0,他引:12
Jaromczyk J.W. Toussaint G.T. 《Proceedings of the IEEE. Institute of Electrical and Electronics Engineers》1992,80(9):1502-1517
Results of neighborhood graphs are surveyed. Properties, bounds on the size, algorithms, and variants of the neighborhood graphs are discussed. Numerous applications including computational morphology, spatial analysis, pattern classification, and databases for computer vision are described 相似文献
5.
There exist many ideas and assumptions about the development and meaning of modularity in biological and technical neural systems. We empirically study the evolution of connectionist models in the context of modular problems. For this purpose, we define quantitative measures for the degree of modularity and monitor them during evolutionary processes under different constraints. It turns out that the modularity of the problem is reflected by the architecture of adapted systems, although learning can counterbalance some imperfection of the architecture. The demand for fast learning systems increases the selective pressure towards modularity. 相似文献
6.
Adolf K. Paul 《电信纪事》1979,34(3-4):154-157
Fourier analysis of a finite time series allocates amplitudes and phases to a given set of frequencies which are integer multiples of a basic frequency determined by the length of the time series. Anharmonic frequency analysis (Afa)collects the spectral components between those frequencies and the results are the average amplitude and phase for each band defined by two consecutive frequencies. A repetition of the transform involved for a later reference time determines the effective frequency within each band by the time change of the phase. This process is by far superior to Fourier analysis especially if unknown discrete frequencies are present in the time series and those frequencies are not harmonics of one common basic frequency. The principles of this method, some of its properties and a few results are presented. 相似文献
7.
无铅无乙基硫脲氯醚橡胶硫化体系的开发 总被引:1,自引:0,他引:1
由于健康和环境方面的原因,在欧洲,氯醚橡胶是不允许使用含铅和乙基硫脲的硫化体系的。因此,研发无铅无乙基硫脲的硫化体系迫在眉睫。通过使用特殊的商业化的材料,可使氯醚混炼胶的物理机械性能、耐热老化性、耐燃油性能、贮存稳定性及成本均达到或超过含铅硫化体系的氯醚混炼胶。新研发的无铅无乙基硫脲硫化体系氯醚胶的贮存期至少可以达到7周。同时,这些无铅无乙基硫脲硫化体系氯醚胶的材料成本与含铅硫化体系的相当。 相似文献
8.
Kanitz Hans Hawelka Schormüller Schwaibold Griebel W. Schreiber Beckel Kortenhaus H. Wenzl Lars Erlandsen Klinc Patzsch Bäurle Diemair Adolf Hanak A. Lendle Pawletta Zacher R. Grau K. Höll Schloemer Jesser W. Wodsak Lindner Masling Haevecker Steinbeck J. Großfeld E. Tornow K. Müller W. Ludorff W. Hämmerle Willy Lindner Brüning O. Windhausen E. Dinslage C. Pyriki Reichard R. Strohecker Spitta Kluge Krzywanek R. Nitsche 《European Food Research and Technology》1942,84(3):230-288
9.
Andrej Kovic Andrej Znidarsic Adolf Jesih Ales Mrzel Miran Gaberscek Abdou Hassanien 《Nanoscale research letters》2012,7(1):567
We describe a straightforward technique to synthesize pure Mo nanowires (NWs) from Mo6SyIz (8,2 <y + z ≤ 10) NWs as precursor templates. The structural transformations occur when Mo6SyIz NWs are annealed in Ar/H2 mixture leading to the formation of pure Mo NWs with similar structures as initial morphologies. Detailed microscopic characterizations show that large diameters (>15 nm) Mo NWs are highly porous, while small diameters (<7 nm) are made of solid nanocrystalline grains. We find NW of diameter 4 nm can carry up to 30 μA current without suffering structural degradation. Moreover, NWs can be elastically deformed over several cycles without signs of plastic deformation. 相似文献
10.
Hannelore T. Koller Torsten Klade Adolf Ellinger Michael Breitenbach 《Yeast (Chichester, England)》1996,12(1):53-65
We isolated the Saccharomyces cerevisiae GRC5 (gr owth control) gene by functional complementation in vivo of a ts (t emperature s ensitive) mutation. Phenotypic analysis suggested involvement of GRC5 in cell growth and proliferation. Mutant cells arrest their cell cycles after one to three cell divisions predominantly as mother cells with a large bud. In the region of the septum, a massive accumulation of cell wall material is observed. The mother and daughter nuclei are well separated and spindles are no longer present, while the cytoskeleton is of aberrant appearance. Arrested cells do not perform protein synthesis and are unable to mate. Furthermore, grc5-1ts cells rapidly lose viability at the restrictive temperature (37°C) only on full media, but not under nitrogen-starvation conditions, indicating that proper response to this nutrient limitation is still intact in mutant cells after cell cycle arrest. The sequence of GRC5 translates into a basic protein of 221 amino acids with a corresponding Mr of 25·4 kDa. GRC5 is a member of the highly conserved QM gene family, members of which have been reported from plants, invertebrates and vertebrates. The amino acid sequence of GRC5 over its entire length is more than 60% identical to the human QM protein, expression of which is associated with loss of the tumorigenic phenotype in a cell line derived from Wilms' tumor, a malignancy of the embyronic kidney. Here, we show that GRC5 is an essential yeast gene, the function of which as inferred from analysis of the grc5-1ts mutant is crucial for establishment of proper cytoskeletal structure and regulation of growth in yeast cells. 相似文献