Transporters for cationic amino acids in animal cells: discovery, structure, and function

The structure and function of the four cationic amino acid transporters identified in animal cells are discussed. The systems differ in specificity, cation dependence, and physiological role. One of them, system y+, is selective for cationic amino acids, whereas the others (B[0,+], b[0,+], and y+ L) also accept neutral amino acids. In recent years, cDNA clones related to these activities have been isolated. Thus two families of proteins have been identified: 1) CAT or cationic amino acid transporters and 2) BAT or broad-scope transport proteins. In the CAT family, three genes encode for four different isoforms [CAT-1, CAT-2A, CAT-2(B) and CAT-3]; these are approximately 70-kDa proteins with multiple transmembrane segments (12-14), and despite their structural similarity, they differ in tissue distribution, kinetics, and regulatory properties. System y+ is the expression of the activity of CAT transporters. The BAT family includes two isoforms (rBAT and 4F2hc); these are 59- to 78-kDa proteins with one to four membrane-spanning segments, and it has been proposed that these proteins act as transport regulators. The expression of rBAT and 4F2hc induces system b[0,+] and system y+ L activity in Xenopus laevis oocytes, respectively. The roles of these transporters in nutrition, endocrinology, nitric oxide biology, and immunology, as well as in the genetic diseases cystinuria and lysinuric protein intolerance, are reviewed. Experimental strategies, which can be used in the kinetic characterization of coexpressed transporters, are also discussed.     

Leveraging 802.11n frame aggregation to enhance QoS and power consumption in Wi-Fi networks

The Wi-Fi technology, driven by its tremendous success, is expanding into a wide variety of devices and applications. However, many of these new devices, like handheld devices, pose new challenges in terms of QoS and energy efficiency. In order to address these challenges, in this paper we study how the novel MAC aggregation mechanisms developed in the 802.11n standard can be used to enhance the current 802.11 QoS and power saving protocols. Our contribution is twofold. First, we present a simulation study that illustrates the interactions between 802.11n and the current 802.11 QoS and power saving protocols. This study reveals that the 802.11n MAC aggregation mechanisms perform better when combined with the power save mode included in the original 802.11 standard than with the 802.11e U-APSD protocol. Second, we design CA-DFA, an algorithm that, using only information available at layer two, adapts the amount of 802.11n aggregation used by a Wi-Fi station according to the level of congestion in the network. A detailed performance evaluation demonstrates the benefits of CA-DFA in terms of QoS, energy efficiency and network capacity with respect to state of the art alternatives.     

Cover Feature: Superior Proton-Transfer Catalytic Promiscuity of Cytochrome c in Self-Organized Media (7/2021)

Evolutionarily elderly proteins commonly feature greater catalytic promiscuity. Cytochrome c is among the first set of proteins in evolution to have known prospects in electron transport and peroxidative properties. Here, we report that cyt c is also a proficient proton-transfer catalyst and enhances the Kemp elimination (KE; model reaction to show proton transfer catalytic property) by ∼750-fold on self-organized systems like micelles and vesicles. The self-organized systems mimic the mitochondrial environment in vitro for cyt c. Using an array of biophysical and biochemical mutational assays, both acid–base and redox mechanistic pathways have been explored. The histidine moiety close to hemin group (His18) is mainly responsible for proton abstraction to promote the concerted E2 pathway for KE catalysis when cyt c is in its oxidized form; this has also been confirmed by a H18A mutant of cyt c. However, the redox pathway is predominant under reducing conditions in the presence of dithiothreitol over the pH range 6–7.4. Interestingly, we found almost 750-fold enhanced KE catalysis by cyt c compared to aqueous buffer. Overall, in addition to providing mechanistic insights, the data reveal an unprecedented catalytic property of cyt c that could be of high importance in an evolutionary perspective considering its role in delineating the phylogenic tree and also towards generating programmable designer biocatalysts.     

Effect of process parameters and optimization for photochemical machining of brass and german silver

This article focuses on parametric optimization for photochemical machining (PCM) of brass and german silver. The aim of the study is to analyze the effect of control parameters on response measures, that is, surface roughness, material removal rate, and edge deviation and optimization of parameters considering different weight percentage for each performance measure. The control parameters have been selected as etchant concentration, etching temperature, and etching time. Using full factorial method of design of experiments, PCM has been carried out using ferric chloride as etchant. Surface roughness and edge deviation should be less, while material removal rate is desired high. For satisfying this multi-objective condition, overall evaluation criteria (OEC) have been formulated by assigning different and equal weight percentage to response measures. The optimized condition for particular OEC is obtained, and analysis of variance (ANOVA) has been performed for observing effect of control parameters on response measures. Surface topography study has been performed using scanning electron microscopy, and material composition analysis has been carried out using energy dispersive spectroscopy. The surface roughness is observed lower for brass, while the edge deviation is found lesser for german silver. The material removal rate is observed higher for brass compared to german silver.     

Crawl through Neighbors: A Simple Curve Reconstruction Algorithm

Given a planar point set sampled from an object boundary, the process of approximating the original shape is called curve reconstruction. In this paper, a novel non‐parametric curve reconstruction algorithm based on Delaunay triangulation has been proposed and it has been theoretically proved that the proposed method reconstructs the original curve under ε‐sampling. Starting from an initial Delaunay seed edge, the algorithm proceeds by finding an appropriate neighbouring point and adding an edge between them. Experimental results show that the proposed algorithm is capable of reconstructing curves with different features like sharp corners, outliers, multiple objects, objects with holes, etc. The proposed method also works for open curves. Based on a study by a few users, the paper also discusses an application of the proposed algorithm for reconstructing hand drawn skip stroke sketches, which will be useful in various sketch based interfaces.     

On the Corrosion Performance of Monel 400 in Molten LiCl-Li2O-Li at 923 K

Metallurgical and Materials Transactions A - The corrosion resistance of a Ni-Cu alloy, Monel 400, in molten LiCl-Li2O-Li at 923 K (650 °C) was investigated. Exposure testing...     

Simulation of a forced-air-twin-chamber for measuring heat treatment uniformity in harvested tomatoes

A heat transfer simulation model combining convection and conduction was developed to predict accurately the temperatures of air and produce (tomatoes) placed within a custom designed experimental set-up used for the measurement of the uniformity of hot air treatment. The thermal conductivity of the produce and air humidity have significant effects on the uniformity of heat treatment, particularly on the temperature gradients generated in the design. Tomato position, signifying the length of tunnel, is another factor to be considered when designing such experimental device. While air velocity is a factor affecting heat treatment uniformity, the tomato orientation did not show any significant effect. The simulation was experimentally validated and found to be accurate. This method could also be used for other fruits.