实验兔VX2肝肿瘤模型制作及动脉插管技术探讨

目的介绍应用VX2细胞株制作兔移植性肝癌模型,并探讨插管技术的应用.方法实验对象为新西兰大白兔(n=80),VX2瘤块组织接种于肝脏,接种后2～3周实验组(n=51)行剖腹直视下肝动脉直接楔入插管,并作DSA造影.对照组1和2(各10只)分别采取经股动脉-肝动脉插管和开腹经胃十二指肠动脉逆行插管方法对比研究.结果实验组插管成功率为98%(50/51),其中88%(45/51)完成超选择栓塞;对照组1和2的插管成功率分别为30%和40%,只有0/10和4/10完成超选择栓塞.结论移植性兔VX2肝癌是介入治疗实验研究较为理想的动物模型，综合影像评价有利于实验动物的筛选。采用肝动脉细针直接穿刺楔入法插管成功率高，可完成超选择栓塞，是值得推广的实验方法。     

Dopamine-containing gadolinium complex as magnetic resonance imaging contrast agent

Gadolinium diethylenetriamine-pentaacetic bisdopamide (Gd-DTPA-2DA) was synthesized by the incorporation of dopamine to diethylenetriaminepentaacetic acid (DTPA) and further reaction with gadolinium chloride. In vitro and in vivo properties were also evaluated. Gadolinium complex Gd-DTPA-2DA possessed higher relaxation effectiveness and less cytotoxicity to HeLa cells than that of Gd-DTPA. Moreover, Gd-DTPA-2DA greatly enhanced the contrast of MR images of the brains, provided prolonged intravascular duration, and produced highly contrasted visualization of the brain.