Ergovaline and peramine production in endophyte-infected tall fescue: Independent regulation and effects of plant and endophyte genotype

Peramine and ergovaline have ecological and economical significance because they possess insect and/or mammalian toxicity properties. The relationship between these endophytically derived alkaloids in tall fescue (Festuca arundinacea Schreb.) is unknown. Seasonal and plant tissue effects on the concentration of peramine and ergovaline was investigated in field and greenhouse experiments. The relationship between the alkaloids and the regulatory effects of endophyte and plant on their content were investigated among progeny of reciprocal crosses between high- and low-ergovaline and peramine plant-endophyte combinations. Variation in peramine concentration ranged from 750 to 1742 µg/kg in greenhouse-grown plants, and there was no seasonal trend in peramine content. There was a correlation (r=0.69) between peramine and ergovaline content among leaf tissues of field-grown plants, but there was no correlation between the alkaloids in the culm (r=0.20) or panicle (r=0.17) tissues. Mean leaf ergovaline concentration of progeny derived from the low-ergovaline parent (163 µg/kg) was less than the midparent value (228 µg/kg), but mean of progeny from the high-ergovaline parent was not different from the mid-parent value. Ranges within each progeny set were approximately double their mean. Mean leaf peramine concentrations of the progeny sets were similar to the mid-parent value (3354 µg/kg) but ranges were from 1716 to 8753 µg/kg. There was no correlation between leaf peramine and ergovaline (r=0.01). These data suggest that endophytically produced alkaloids are independently regulated and are controlled by both plant and endophyte genotype.     

Computer-assisted techniques to enhance transformative learning in first-year literature courses

When students use computers as learning tools, the whole process of learning, and, indeed, the learners themselves, are transformed. This article illustrates some techniques that foster transformative learning in computer-assisted first-year literature classes: first, a lesson plan on A Valediction: Forbidding Mourning that uses Microsoft Word functions, including format painter, tables, and annotation to explore meaning in context; second, a plan for learners to use subconference options in the Daedalus Interactive Writing Environment to analyze Oedipus Rex; finally, a demonstration of how students engage in a meta-reflection process as they explore Barn Burning with Freelance Graphics.Marguerite Jamieson is an English instructor at Anne Arundel Community College in Arnold, Maryland, and a doctoral student at George Mason University. Her research interests include forming bridges between adult learning theory and contemporary literary theory — especially drawing on transformational learning theory and the work of Mikhail Bakhtin and Lev Vygotsky.Rebecca Kajs holds a doctorate in English from Texas Woman's University with a concentration in rhetoric. For ten years, she taught the use of heuristic tools for reading analysis at the University of Texas at Arlington. She is currently an associate professor of English and Philosophy at Anne Arundel Community College.Anne Agee holds a doctorate in rhetoric from The Catholic University of America. A professor of English and formerly director of the Humanities Computer Center at Anne Arundel Community College, she is currently the college's Coordinator of Instructional Technology. Dr. Agee and Professor Jamieson have collaborated in a study of the learning environment in a computer classroom, the results of which were published in the Fall 1995 issue of Teaching/Learning Conversations. Dr. Agee has also published Using [Daedalus] InterChange as a Teachers' Journal in the Fall 1995 issue of Wings.     

Comparisons of the RETRAN two-phase flow model with experimental data

The RETRAN computer program was designed for thermal-hydraulic analysis of nuclear steam supply systems at steady-state and off-normal conditions. A homogeneous equilibrium mixture model (HEMM) of two-phase flow is used in the program. The constitutive models for wall friction, two-phase friction multiplier, heat transfer coefficient, and critical heat flux are compared with steady-state experimental data in this report. The void fraction predicted by the HEMM is also compared with data. The results of the data comparisons indicate that the constitutive models are accurate representations and that the RETRAN numerical scheme provides an excellent framework for data comparisons.     

Isopropyl alcohol as a solvent for free fatty acid titration

George H. Kyser of Barrow-Agee Laboratories, Inc., Cairo, Illinois, suggested the possibility of using Isopropyl Alcohol as a substitute for Specially Denatured Alcohol, Formula No. 30, in the titration of free fatty acids in crude oil and in the oil of cottonseed samples. This substitution would eliminate the necessity of special permits and bonds for the use of alcohol. Isopropyl Alcohol and cotonseed oil are miscible in all proportions, thus simplifying the titration without the use of violent shaking. Isopropyl Alcohol, with the indicator, is added direct to the oil in the titrating flask and the titration made simply by a twirling motion of the flask. A series of comparative results indicate a very close agreement between titrations, using both Specially Denatured Alcohol and Isopropyl Alcohol as solvents. The author suggests that a committee make further study and recommendations on the use of Isopropyl Alcohol as a solvent.     

RETRAN's improved small break modeling capability

The RETRAN-02 computer code has been used extensively to analyze both operational transients and small break loss-of-coolant accidents (SBLOCA). While the results of these analyses are generally in good agreement with data, the explicit solution method sometimes requires small time-steps for portions of the small break calculations. The RETRAN-03 code, now under development, is incorporating additional models to extend the capability of RETRAN-02 and improve the code efficiency for SBLOCA's. Included in these models is a stable, implicit solution method which has provided a significant reduction in CPU requirements while maintaining satisfactory analyses results. The new models for RETRAN-03 and results of small break analyses using RETRAN-03 are briefly discussed in this paper.