GOLPH3 Regulates EGFR in T98G Glioblastoma Cells by Modulating Its Glycosylation and Ubiquitylation

Protein trafficking is altered when normal cells acquire a tumor phenotype. A key subcellular compartment in regulating protein trafficking is the Golgi apparatus, but its role in carcinogenesis is still not well defined. Golgi phosphoprotein 3 (GOLPH3), a peripheral membrane protein mostly localized at the trans-Golgi network, is overexpressed in several tumor types including glioblastoma multiforme (GBM), the most lethal primary brain tumor. Moreover, GOLPH3 is currently considered an oncoprotein, however its precise function in GBM is not fully understood. Here, we analyzed in T98G cells of GBM, which express high levels of epidermal growth factor receptor (EGFR), the effect of stable RNAi-mediated knockdown of GOLPH3. We found that silencing GOLPH3 caused a significant reduction in the proliferation of T98G cells and an unexpected increase in total EGFR levels, even at the cell surface, which was however less prone to ligand-induced autophosphorylation. Furthermore, silencing GOLPH3 decreased EGFR sialylation and fucosylation, which correlated with delayed ligand-induced EGFR downregulation and its accumulation at endo-lysosomal compartments. Finally, we found that EGF failed at promoting EGFR ubiquitylation when the levels of GOLPH3 were reduced. Altogether, our results show that GOLPH3 in T98G cells regulates the endocytic trafficking and activation of EGFR likely by affecting its extent of glycosylation and ubiquitylation.     

Analysis of Combined Systems of Two Endoreversible Engines

A single endoreversible engine can operate as a cooler, a true heat engine, a heat pump, or a refrigerator. We investigate how many different modes of operation a combined system of two endoreversible engines may display. Special attention is paid to the independent combined system which neither consumes nor supplies power.     

Issues in nonlinear stochastic grey box identification

Grey box identification refers to the practice of identifying dynamical systems in model structures exploiting partial prior information. This contribution reviews a method for stochastic grey box identification and surveys experiences and lessons of applying it to a number of industrial processes. Issues to be addressed include advantages and costs of introducing stochastics into the model, the question of what contribution must be expected from the model designer as opposed to what can be formalized in computer algorithms, and an outlook on future plans to resolve present shortcomings.     

阿维斯塔波莱瑞特厂炉卷轧机新技术

阿维斯塔波莱瑞特厂正在进行一个现代化改造项目，以提高炉卷轧机的生产能力，达到年产100万吨。但是，由于在阿维斯塔波莱瑞特厂炉卷轧机上的现有技术已达到极限，只有通过对轧机及自动化系统进行现代化的改造才有可能提高和增强轧机的利用率和易维护性。     

An alcohol expectancy-challenge prevention program for at risk college women.

Alcohol expectancy-challenge programs are effective in changing expectancies and reducing drinking in college men (J. Darker & M. S. Goldman. 1993. 1998); however, recent evidence suggests this protocol might not be effective for women (M. E. Dunn, C. Lau, & I. Y. Cruz, 2000). This study was designed to reevaluate the effectiveness of a social/sexual expectancy-challenge intervention in college women reporting moderate to heavy alcohol use. Forty-six undergraduate women were randomly assigned to the prevention or control condition. Participants reported alcohol expectancies at pre- and posttest and monitored their drinking patterns daily for 6 weeks. The program was effective in changing some expectancies but did not reduce drinking. This further confirms differences in the mechanisms by which expectancy-challenge programs function for men and women. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Prenatal androgens and gender-typed behavior: A study of girls with mild and severe forms of congenital adrenal hyperplasia.

Gender-typed behaviors and interests were investigated in 26 girls, aged 2-10 years, affected with congenital adrenal hyperplasia (CAH) and in 26 unaffected girls matched for age. Girls with CAH were more interested in masculine toys and less interested in feminine toys and were more likely to report having male playmates and to wish for masculine careers. Parents of girls with CAH rated their daughters' behaviors as more boylike than did parents of unaffected girls. A relation was found between disease severity and behavior indicating that more severely affected CAH girls were more interested in masculine toys and careers. No parental influence could be demonstrated on play behavior, nor did the comparison of parents' ratings of wished for behavior versus perceived behavior in their daughters indicate an effect of parental expectations. The results are interpreted as supporting a biological contribution to differences in play behavior between girls with and without CAH. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

An Investigation of the Reduction and Reoxidation of Isolated Vanadate Sites Supported on MCM-48

The reduction and subsequent reoxidation of isolated vanadate species supported on silica was investigated using temperature-programmed reduction and oxidation, along with in-situ XANES and Raman spectroscopy. Approximately 70–80% of the vanadium was reduced to V³⁺ after reduction in H₂ at temperatures up to 923 K. Upon reduction, the vanadyl oxygen was removed and the three remaining V–O bonds are lengthened by 0.2 Å. The vanadate species are rapidly reoxidized when exposed to O₂, with the amount of oxygen uptake matching well with the amount removed during reduction. In-situ Raman spectroscopy during reoxidation in ¹⁸O₂ showed that significant scrambling occurs between gas phase oxygen and surface oxygen species during the reoxidation of the vanadate species.     

The lipid composition of selected tissues from a Mediterranean monk seal,Monachus monachus

The lipid composition of blubber, brain, muscle and heart from a Mediterranean monk sealMonachus monachus (an endangered species) were examined to allow comparisons with more common species of seals. Only neutral lipids (mainly triacylglycerols) were detectable in the blubber lipids, whereas polar lipids predominated in the heart and in the brain. Neutral and polar lipids comprised almost equal proportions in both liver and muscle. Choline glycerophospholipids (CGP) were the major polar lipids, followed by ethanolamine glycerophospholipids (EGP) in the liver, heart and muscle. Cerebrosides accounted for 28.8% of the brain lipids. All lipid classes of the liver contained high levels (31–47%) of polyunsaturated fatty acids (PUFA), with the exception of phosphatidylserine. The total proportion of n−6 PUFA exceeded that of n−3 PUFA in all lipid classes of the liver, due mainly to the high levels of 20∶4n−6. The highest level of 20∶4n−6 occurred in phosphatidylinositol, where it comprised 32.4% of the total fatty acids. The CGP and EGP of the brain contained lower levels of PUFA than those of the liver, muscle and heart. Alkenyl ethers accounted for 35.8% of the total long-chain moieties in brain EGP. The fatty acid composition of blubber triacylglycerols differed from those of the lipid classes from other tissues in that it had a very low ratio of n−6 to n−3 PUFA (0.3) as a result of a lower content of 20∶4n−6.     

Mechanical simulations of SANDIA II tests OECD ISP 48 benchmark

This paper illustrates the work carried out by EDF within the framework of ISP 48 post-test analysis of NUPEC/NRC 1:4-scale model of a prestressed pressure containment vessel of a nuclear power plant [Hessheimer, M.F., Klamerus, E.W., Rightly, G.S., Lambert, L.D., Dameron, R.A., 2003. Overpressurization test of a 1:4-scale prestressed concrete containment vessel model. NUREG/CR-6810, SAND2003-0840P. Sandia National Laboratories, Albuquerque, NM]. EDF as a participant of the International Standard Problem no. 48 [Mathet, E., Hessheimer, M., Ali, S., Tegeler, B., 2005. An international standard problem: analysis of 1:4-scale prestressed concrete containment vessel model under severe accident conditions. Proceedings of 18th International Conference on Structural Mechanics in Reactor Technology. SMiRT 18. Beijing, China] has participated in this program, within the framework of its research and development program on the simulation of non-linear behaviour of nuclear power plant prestressed concrete pressure containment vessels. EDF performed several simulations to determine the ultimate response of the scale model. To determine the most influent parameters in such an analysis several studies were carried out. A full 3D mesh of the entire structure was then created. The mesh was built using a parametric tool to measure the influence of discretization on results. To represent the cracking of concrete, two material laws were then used. The purpose of this paper is to illustrate the ultimate behaviour of SANDIA II model obtained by Code_Aster^® finite element platform, with comparison to tests records, and also to share the lessons learned from the parametric computations and underline precautions that must be taken in such studies.