Uncertainties in the determination of pyrrolizidine alkaloid levels in naturally contaminated honeys and comparison of results obtained by different analytical approaches

The contamination of honey with hepatotoxic pyrrolizidine alkaloids (PAs) is a well-known hazard for food safety. While management strategies and controls of the honey industry aim to reduce the PA levels, uncertainties remain with regard to the safety of regionally produced and marketed honey. In addition, a previous study showed large differences of results obtained after various periods of storage and apparent differences between the analytical results of different laboratories. Therefore, this study aimed at examining these uncertainties by monitoring the impact of storage on the PA and PA N-oxide (PANO) content of two freshly harvested honeys and on possible demixing effects caused by pollen settling. Additionally, three analytical approaches – target analysis with matrix-matched calibration or standard addition and a sum parameter method – were applied for a comparative analysis of 20 honeys harvested in summer 2016. All samples originated from Schleswig-Holstein in Northern Germany where the PA plant Jacobaea vulgaris is currently observed on a massive scale. The results of the time series analyses showed that PANO levels markedly decreased within a few weeks and practically reached the LOD 16 weeks after harvest. Tertiary PAs, by contrast, remained stable and did not increase as a consequence of PANO decrease. The experiments on a putative demixing, which may result in a heterogeneous distribution of PAs/PANOs, revealed that there was no such effect during storage of up to 12 weeks. A comparison of the PA/PANO levels obtained by different analytical approaches showed that in some cases the sum parameter method yielded much higher levels than the target approaches, whereas in other cases, the target analysis with standard addition found higher levels than the other two methods. In summary, the results of this study highlight uncertainties regarding the validity and comparability of analytical results and consequently regarding health risk assessment.     

Mixed Mode VLSI Implementation of a Neural Associative Memory

A mixed mode digital/analog special purpose VLSI hardware implementation of an associative memory with neural architecture is presented. The memory concept is based on a matrix architecture with binary storage elements holding the connection weights. To enhance the processing speed analog circuit techniques are applied to implement the algorithm for the association. To keep the memory density as high as possible two design strategies are considered. First, the number of transistors per storage element is kept to a minimum. In this paper a circuit technique that uses a single 6-transistor cell for weight storage and analog signal processing is proposed. Second, the device precision has been chosen to a moderate level to save area as much as possible. Since device mismatch limits the performance of analog circuits, the impact of device precision on the circuit performance is explicitly discussed. It is shown that the device precision limits the number of rows activated in parallel. Since the input vector as well as the output vector are considered to be sparsely coded it is concluded, that even for large matrices the proposed circuit technique is appropriate and ultra large scale integration with a large number of connection weights is feasible.     

Parabolic dish concentrators approximated by simple surfaces

Two different concentrating mirrors have been constructed that resemble parabolic dish reflectors. Both mirrors are made of slightly curved strips of flat, bendable material. The strips of the most simplified mirror have only large-radius circles and straight lines as boundaries. The necessary equations for making the mirrors are given. Also a simple way to make a stiff, lightweight frame and support for the mirror strips has been developed. Models of the mirrors have been built and successfully used for cooking and baking.     

Ammoxidation of toluene over Y-Ba-Cu-Co-O perovskites

Ammoxidation of toluene over the perovskites YBa₂Cu₃O_6.1, YBa₂Cu₂CoO_6.7 and YBaCuCoO_4.9 was investigated at 400 °C. At low partial pressures of O₂ benzonitrile was selectively formed, while CO₂ was the main product at high pressures of O₂. Systematic differences in activity were observed for the three phases and are related to the crystal contents of Cu and Co. At low O₂ pressures, Cu-sites are active for nitrile formation, while Co-sites give CO₂. At high O₂ pressures, the activity for CO₂ of Cu-sites increases more than that of Co-sites due to filling of near-surface oxygen vacancies.     

Interfacing remote transaction services using UPnP

Transaction execution in mobile environments needs to be flexible, not only to support typical mobile computing characteristics, like movement, disconnections and limited resources, but also to support the variety of transactional properties that might be required by different applications. Existing models for mobile transaction management solve different aspects of transaction execution, but are not flexible enough to solve all required aspects. Instead of designing a new transaction model, we propose a middleware (MobileTSe) which utilize existing transaction models to handle various requirements for mobile transaction execution. This paper presents an approach for flexible transaction processing in mobile applications, and describes how MobileTSe makes transaction services with different properties available on mobile units. We suggest a solution with transaction service discovery and control using UPnP.     

Evalvid-RA: trace driven simulation of rate adaptive MPEG-4 VBR video

Due to the increasing deployment of conversational real-time applications like VoIP and videoconferencing, the Internet is today facing new challenges. Low end-to-end delay is a vital QoS requirement for these applications, and the best effort Internet architecture does not support this natively. The delay and packet loss statistics are directly coupled to the aggregated traffic characteristics when link utilization is close to saturation. In order to investigate the behavior and quality of such applications under heavy network load, it is therefore necessary to create genuine traffic patterns. Trace files of real compressed video and audio are text files containing the number of bytes per video and audio frame. These can serve as material to construct mathematical traffic models. They can also serve as traffic generators in network simulators since they determine the packet sizes and their time schedule. However, to inspect perceived quality, the compressed binary content is needed to ensure decoding of received media. The EvalVid streaming video tool-set enables this using a sophisticated reassembly engine. Nevertheless, there has been a lack of research solutions for rate adaptive media content. The Internet community fears a congestion collapse if the usage of non-adaptive media content continues to grow. This paper presents a solution named Evalvid-RA for the simulation of true rate adaptive video. The solution generates real rate adaptive MPEG-4 streaming traffic, using the quantizer scale for adjusting the sending rate. A feedback based VBR rate controller is used at simulation time, supporting TFRC and a proprietary congestion control system named P-AQM. Example ns-2 simulations of TFRC and P-AQM demonstrate Evalvid-RA’s capabilities in performing close-to-true rate adaptive codec operation with low complexity to enable the simulation of large networks with many adaptive media sources on a single computer.     

Solders development and application process for a micro chip-camera

Nowadays, micro components have to fulfill rising optical requirements for different scientific and industrial fields like astronomy, medicine or multimedia. For this purpose, advanced miniaturized chip-cameras are produced for the microsystems engineering market. The assembly and joining technologies play a very important role in the production of these micro components. Several challenges are associated with the joining of chip-cameras. In this study, the application of the soldering technology has been considered in order to face these challenges. Two joining technologies have been investigated: active soldering and transient liquid phase (TLP) bonding. Both soldering processes have shown a big potential for hybrid microsystems joining in previous studies. For both processes, soldering alloys and parameters have been conceived in order to fulfill the joining requirements of the micro camera components. For instance, the joining temperature represents a major challenge because the chip-camera consists of a plastic material, polymethyl methacrylate (PMMA). Therefore, particular attention has been directed to the soldering as well as to the coating temperature. The experimental investigations concerning the solders application through physical vapor deposition (PVD) have been supported by finite element method (FEM) simulations. The analysis of  the temperature distribution in the micro components during the coating process was the focus of  the calculations. Possible undesirable local overheated areas of the chip-camera components can be detected through simulation.

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